Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution

Autores
Andreotti, Carolina Soledad; Baravalle, Celina; Sacco, Sofía Clara; Lovato, Melisa Belen; Pereyra, Elizabet Amanda Lorena; Renna, Maria Sol; Ortega, Hugo Hector; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to characterize the immune response in Staphylococcus aureus chronicallyinfected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows inlate lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections(IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milkingand samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2(TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls atthe three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-á),interleukin (IL)-1á and IL-17 was significantly affected by IMI; being higher in S. aureus-infectedthan uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quartersprotein expression of lactoferrin increased from day 7 to 14 of involution, decreasing significantly today 21 in mammary quarters with chronic infections. The number of monocytes-macrophages wassignificantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d ofinvolution. The number of T lymphocytes was significantly higher in S. aureus-infected than inuninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantlyhigher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing aprogressive increase as involution advanced. These results demonstrated a sustained and exacerbatedinnate and adaptive immune response during chronic S. aureus IMI, playing a critical role in theinfection control during active involution
Fil: Andreotti, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lovato, Melisa Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina
Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Immune Response
S. Aureus
Mastitis
Involution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41035
Acceder
id CONICETDig_1b821df465cee1e574eaeffd954faeeb
oai_identifier_str oai:ri.conicet.gov.ar:11336/41035
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involutionAndreotti, Carolina SoledadBaravalle, CelinaSacco, Sofía ClaraLovato, Melisa BelenPereyra, Elizabet Amanda LorenaRenna, Maria SolOrtega, Hugo HectorCalvinho, Luis FernandoDallard, Bibiana ElisabetImmune ResponseS. AureusMastitisInvolutionhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to characterize the immune response in Staphylococcus aureus chronicallyinfected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows inlate lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections(IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milkingand samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2(TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls atthe three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-á),interleukin (IL)-1á and IL-17 was significantly affected by IMI; being higher in S. aureus-infectedthan uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quartersprotein expression of lactoferrin increased from day 7 to 14 of involution, decreasing significantly today 21 in mammary quarters with chronic infections. The number of monocytes-macrophages wassignificantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d ofinvolution. The number of T lymphocytes was significantly higher in S. aureus-infected than inuninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantlyhigher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing aprogressive increase as involution advanced. These results demonstrated a sustained and exacerbatedinnate and adaptive immune response during chronic S. aureus IMI, playing a critical role in theinfection control during active involutionFil: Andreotti, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Lovato, Melisa Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; ArgentinaFil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevier2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41035Andreotti, Carolina Soledad; Baravalle, Celina; Sacco, Sofía Clara; Lovato, Melisa Belen; Pereyra, Elizabet Amanda Lorena; et al.; Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 54; 10-2017; 51-600147-9571CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147957117300759info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2017.08.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:15Zoai:ri.conicet.gov.ar:11336/41035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:15.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
title Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
spellingShingle Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
Andreotti, Carolina Soledad
Immune Response
S. Aureus
Mastitis
Involution
title_short Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
title_full Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
title_fullStr Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
title_full_unstemmed Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
title_sort Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution
dc.creator.none.fl_str_mv Andreotti, Carolina Soledad
Baravalle, Celina
Sacco, Sofía Clara
Lovato, Melisa Belen
Pereyra, Elizabet Amanda Lorena
Renna, Maria Sol
Ortega, Hugo Hector
Calvinho, Luis Fernando
Dallard, Bibiana Elisabet
author Andreotti, Carolina Soledad
author_facet Andreotti, Carolina Soledad
Baravalle, Celina
Sacco, Sofía Clara
Lovato, Melisa Belen
Pereyra, Elizabet Amanda Lorena
Renna, Maria Sol
Ortega, Hugo Hector
Calvinho, Luis Fernando
Dallard, Bibiana Elisabet
author_role author
author2 Baravalle, Celina
Sacco, Sofía Clara
Lovato, Melisa Belen
Pereyra, Elizabet Amanda Lorena
Renna, Maria Sol
Ortega, Hugo Hector
Calvinho, Luis Fernando
Dallard, Bibiana Elisabet
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immune Response
S. Aureus
Mastitis
Involution
topic Immune Response
S. Aureus
Mastitis
Involution
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to characterize the immune response in Staphylococcus aureus chronicallyinfected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows inlate lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections(IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milkingand samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2(TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls atthe three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-á),interleukin (IL)-1á and IL-17 was significantly affected by IMI; being higher in S. aureus-infectedthan uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quartersprotein expression of lactoferrin increased from day 7 to 14 of involution, decreasing significantly today 21 in mammary quarters with chronic infections. The number of monocytes-macrophages wassignificantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d ofinvolution. The number of T lymphocytes was significantly higher in S. aureus-infected than inuninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantlyhigher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing aprogressive increase as involution advanced. These results demonstrated a sustained and exacerbatedinnate and adaptive immune response during chronic S. aureus IMI, playing a critical role in theinfection control during active involution
Fil: Andreotti, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lovato, Melisa Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina
Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description The aim of this study was to characterize the immune response in Staphylococcus aureus chronicallyinfected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows inlate lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections(IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milkingand samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2(TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls atthe three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-á),interleukin (IL)-1á and IL-17 was significantly affected by IMI; being higher in S. aureus-infectedthan uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quartersprotein expression of lactoferrin increased from day 7 to 14 of involution, decreasing significantly today 21 in mammary quarters with chronic infections. The number of monocytes-macrophages wassignificantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d ofinvolution. The number of T lymphocytes was significantly higher in S. aureus-infected than inuninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantlyhigher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing aprogressive increase as involution advanced. These results demonstrated a sustained and exacerbatedinnate and adaptive immune response during chronic S. aureus IMI, playing a critical role in theinfection control during active involution
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41035
Andreotti, Carolina Soledad; Baravalle, Celina; Sacco, Sofía Clara; Lovato, Melisa Belen; Pereyra, Elizabet Amanda Lorena; et al.; Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 54; 10-2017; 51-60
0147-9571
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41035
identifier_str_mv Andreotti, Carolina Soledad; Baravalle, Celina; Sacco, Sofía Clara; Lovato, Melisa Belen; Pereyra, Elizabet Amanda Lorena; et al.; Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 54; 10-2017; 51-60
0147-9571
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147957117300759
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cimid.2017.08.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268653277413376
score 13.13397

Publicaciones similares