Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data
- Autores
- Gómez Palacio, Andrés; Morinaga, Gen; Turner, Paul E.; Micieli, Maria Victoria; Elnour, Mohammed Ahmed B.; Salim, Bashir; Surendran, Sinnathamby Noble; Ramasamy, Ranjan; Powell, Jeffrey R.; Soghigian, John; Gloria Soria, Andrea
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The mosquito Aedes aegypti is the primary vector of many human arboviruses such as dengue, yellow fever, chikungunya, and Zika, which affect millions of people worldwide. Population genetic studies on this mosquito have been important in understanding its invasion pathways and success as a vector of human disease. The Axiom aegypti1 SNP chip was developed from a sample of geographically diverse A. aegypti populations to facilitate genomic studies on this species. We evaluate the utility of the Axiom aegypti1 SNP chip for population genetics and compare it with a low-depth shotgun sequencing approach using mosquitoes from the native (Africa) and invasiveranges (outside Africa). These analyses indicate that results from the SNP chip are highly reproducible and have a higher sensitivity to capture alternative alleles than a low-coverage whole-genome sequencing approach. Although the SNP chip suffers from ascertainment bias, results from population structure, ancestry, demographic, and phylogenetic analyses using the SNP chip were congruent with those derived from low-coverage whole-genome sequencing, and consistent with previous reports on Africa and outside Africa populations using microsatellites. More importantly, we identified a subset of SNPs that can be reliably used to generate merged databases,opening the door to combined analyses. We conclude that the Axiom aegypti1 SNP chip is a convenient, more accurate, low-cost alternative to low-depth whole-genome sequencing for population genetic studies of A. aegypti that do not rely on full allelic frequency spectra.Whole-genome sequencing and SNP chip data can be easily merged, extending the usefulness of both approaches.
Fil: Gómez Palacio, Andrés. Universidad Pedagógica y Tecnológica de Colombia; Colombia
Fil: Morinaga, Gen. University of Calgary; Canadá
Fil: Turner, Paul E.. University of Yale; Estados Unidos
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Elnour, Mohammed Ahmed B.. Tropical Medicine Research Institute; Sudán
Fil: Salim, Bashir. University of Khartoum; Sudán
Fil: Surendran, Sinnathamby Noble. University of Jaffna; Sri Lanka
Fil: Ramasamy, Ranjan. University of Jaffna; Sri Lanka
Fil: Powell, Jeffrey R.. University of Yale; Estados Unidos
Fil: Soghigian, John. University of Calgary; Canadá
Fil: Gloria Soria, Andrea. Universidad Pedagógica y Tecnológica de Colombia; Colombia - Materia
-
genotyping array
SNP chip
Aedes aegypti
population structure - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/267319
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/267319 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing dataGómez Palacio, AndrésMorinaga, GenTurner, Paul E.Micieli, Maria VictoriaElnour, Mohammed Ahmed B.Salim, BashirSurendran, Sinnathamby NobleRamasamy, RanjanPowell, Jeffrey R.Soghigian, JohnGloria Soria, Andreagenotyping arraySNP chipAedes aegyptipopulation structurehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The mosquito Aedes aegypti is the primary vector of many human arboviruses such as dengue, yellow fever, chikungunya, and Zika, which affect millions of people worldwide. Population genetic studies on this mosquito have been important in understanding its invasion pathways and success as a vector of human disease. The Axiom aegypti1 SNP chip was developed from a sample of geographically diverse A. aegypti populations to facilitate genomic studies on this species. We evaluate the utility of the Axiom aegypti1 SNP chip for population genetics and compare it with a low-depth shotgun sequencing approach using mosquitoes from the native (Africa) and invasiveranges (outside Africa). These analyses indicate that results from the SNP chip are highly reproducible and have a higher sensitivity to capture alternative alleles than a low-coverage whole-genome sequencing approach. Although the SNP chip suffers from ascertainment bias, results from population structure, ancestry, demographic, and phylogenetic analyses using the SNP chip were congruent with those derived from low-coverage whole-genome sequencing, and consistent with previous reports on Africa and outside Africa populations using microsatellites. More importantly, we identified a subset of SNPs that can be reliably used to generate merged databases,opening the door to combined analyses. We conclude that the Axiom aegypti1 SNP chip is a convenient, more accurate, low-cost alternative to low-depth whole-genome sequencing for population genetic studies of A. aegypti that do not rely on full allelic frequency spectra.Whole-genome sequencing and SNP chip data can be easily merged, extending the usefulness of both approaches.Fil: Gómez Palacio, Andrés. Universidad Pedagógica y Tecnológica de Colombia; ColombiaFil: Morinaga, Gen. University of Calgary; CanadáFil: Turner, Paul E.. University of Yale; Estados UnidosFil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Elnour, Mohammed Ahmed B.. Tropical Medicine Research Institute; SudánFil: Salim, Bashir. University of Khartoum; SudánFil: Surendran, Sinnathamby Noble. University of Jaffna; Sri LankaFil: Ramasamy, Ranjan. University of Jaffna; Sri LankaFil: Powell, Jeffrey R.. University of Yale; Estados UnidosFil: Soghigian, John. University of Calgary; CanadáFil: Gloria Soria, Andrea. Universidad Pedagógica y Tecnológica de Colombia; ColombiaOxford University Press2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267319Gómez Palacio, Andrés; Morinaga, Gen; Turner, Paul E.; Micieli, Maria Victoria; Elnour, Mohammed Ahmed B.; et al.; Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data; Oxford University Press; G3: Genes, Genomes, Genetics; 14; 6; 4-2024; 1-122160-1836CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkae082/7646798info:eu-repo/semantics/altIdentifier/doi/10.1093/g3journal/jkae082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:06Zoai:ri.conicet.gov.ar:11336/267319instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:06.676CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
title |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
spellingShingle |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data Gómez Palacio, Andrés genotyping array SNP chip Aedes aegypti population structure |
title_short |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
title_full |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
title_fullStr |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
title_full_unstemmed |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
title_sort |
Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data |
dc.creator.none.fl_str_mv |
Gómez Palacio, Andrés Morinaga, Gen Turner, Paul E. Micieli, Maria Victoria Elnour, Mohammed Ahmed B. Salim, Bashir Surendran, Sinnathamby Noble Ramasamy, Ranjan Powell, Jeffrey R. Soghigian, John Gloria Soria, Andrea |
author |
Gómez Palacio, Andrés |
author_facet |
Gómez Palacio, Andrés Morinaga, Gen Turner, Paul E. Micieli, Maria Victoria Elnour, Mohammed Ahmed B. Salim, Bashir Surendran, Sinnathamby Noble Ramasamy, Ranjan Powell, Jeffrey R. Soghigian, John Gloria Soria, Andrea |
author_role |
author |
author2 |
Morinaga, Gen Turner, Paul E. Micieli, Maria Victoria Elnour, Mohammed Ahmed B. Salim, Bashir Surendran, Sinnathamby Noble Ramasamy, Ranjan Powell, Jeffrey R. Soghigian, John Gloria Soria, Andrea |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
genotyping array SNP chip Aedes aegypti population structure |
topic |
genotyping array SNP chip Aedes aegypti population structure |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The mosquito Aedes aegypti is the primary vector of many human arboviruses such as dengue, yellow fever, chikungunya, and Zika, which affect millions of people worldwide. Population genetic studies on this mosquito have been important in understanding its invasion pathways and success as a vector of human disease. The Axiom aegypti1 SNP chip was developed from a sample of geographically diverse A. aegypti populations to facilitate genomic studies on this species. We evaluate the utility of the Axiom aegypti1 SNP chip for population genetics and compare it with a low-depth shotgun sequencing approach using mosquitoes from the native (Africa) and invasiveranges (outside Africa). These analyses indicate that results from the SNP chip are highly reproducible and have a higher sensitivity to capture alternative alleles than a low-coverage whole-genome sequencing approach. Although the SNP chip suffers from ascertainment bias, results from population structure, ancestry, demographic, and phylogenetic analyses using the SNP chip were congruent with those derived from low-coverage whole-genome sequencing, and consistent with previous reports on Africa and outside Africa populations using microsatellites. More importantly, we identified a subset of SNPs that can be reliably used to generate merged databases,opening the door to combined analyses. We conclude that the Axiom aegypti1 SNP chip is a convenient, more accurate, low-cost alternative to low-depth whole-genome sequencing for population genetic studies of A. aegypti that do not rely on full allelic frequency spectra.Whole-genome sequencing and SNP chip data can be easily merged, extending the usefulness of both approaches. Fil: Gómez Palacio, Andrés. Universidad Pedagógica y Tecnológica de Colombia; Colombia Fil: Morinaga, Gen. University of Calgary; Canadá Fil: Turner, Paul E.. University of Yale; Estados Unidos Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Elnour, Mohammed Ahmed B.. Tropical Medicine Research Institute; Sudán Fil: Salim, Bashir. University of Khartoum; Sudán Fil: Surendran, Sinnathamby Noble. University of Jaffna; Sri Lanka Fil: Ramasamy, Ranjan. University of Jaffna; Sri Lanka Fil: Powell, Jeffrey R.. University of Yale; Estados Unidos Fil: Soghigian, John. University of Calgary; Canadá Fil: Gloria Soria, Andrea. Universidad Pedagógica y Tecnológica de Colombia; Colombia |
description |
The mosquito Aedes aegypti is the primary vector of many human arboviruses such as dengue, yellow fever, chikungunya, and Zika, which affect millions of people worldwide. Population genetic studies on this mosquito have been important in understanding its invasion pathways and success as a vector of human disease. The Axiom aegypti1 SNP chip was developed from a sample of geographically diverse A. aegypti populations to facilitate genomic studies on this species. We evaluate the utility of the Axiom aegypti1 SNP chip for population genetics and compare it with a low-depth shotgun sequencing approach using mosquitoes from the native (Africa) and invasiveranges (outside Africa). These analyses indicate that results from the SNP chip are highly reproducible and have a higher sensitivity to capture alternative alleles than a low-coverage whole-genome sequencing approach. Although the SNP chip suffers from ascertainment bias, results from population structure, ancestry, demographic, and phylogenetic analyses using the SNP chip were congruent with those derived from low-coverage whole-genome sequencing, and consistent with previous reports on Africa and outside Africa populations using microsatellites. More importantly, we identified a subset of SNPs that can be reliably used to generate merged databases,opening the door to combined analyses. We conclude that the Axiom aegypti1 SNP chip is a convenient, more accurate, low-cost alternative to low-depth whole-genome sequencing for population genetic studies of A. aegypti that do not rely on full allelic frequency spectra.Whole-genome sequencing and SNP chip data can be easily merged, extending the usefulness of both approaches. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/267319 Gómez Palacio, Andrés; Morinaga, Gen; Turner, Paul E.; Micieli, Maria Victoria; Elnour, Mohammed Ahmed B.; et al.; Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data; Oxford University Press; G3: Genes, Genomes, Genetics; 14; 6; 4-2024; 1-12 2160-1836 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/267319 |
identifier_str_mv |
Gómez Palacio, Andrés; Morinaga, Gen; Turner, Paul E.; Micieli, Maria Victoria; Elnour, Mohammed Ahmed B.; et al.; Robustness in population-structure and demographic-inference results derived from the Aedes aegypti genotyping chip and whole-genome sequencing data; Oxford University Press; G3: Genes, Genomes, Genetics; 14; 6; 4-2024; 1-12 2160-1836 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkae082/7646798 info:eu-repo/semantics/altIdentifier/doi/10.1093/g3journal/jkae082 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |