Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes

Autores
Ochoa, Maria Judith; González Flores, L. M.; Cruz Rubio, J. M.; Rivera López, L. A.; Rodriguez, Sergio A.; Nazareno, Mónica Azucena; Gómez Leyva, J. F.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Black spot disease, caused by the hemibiotrophic fungus Pseudocercospora opuntiae, is one of the main phytosanitary problems of cactus (Opuntia spp.). Through mass selection, one cultivar of Opuntia ficus-indica (L.) Mill. resistant to colonization by P. opuntiae was identified. The ethanolic extract of resistant cladodes showed higher levels of total condensed tannins, flavonoids and polyphenols than those of the susceptible genotypes, generating 93% inhibition of P. opuntiae conidial germination in vitro. The total protein in the resistant genotype showed 300% higher β-1,3-glucanase than the susceptible genotype. This increased activity was able to inhibit germination of conidia by 90%, a similar effect to that of the fungicide Captan® (N‑trichloromethylthio-4-cyclohexene 1,2-dicarboximide). It was shown, for the first time, that the combined action of cactus polyphenols and β-1,3-glucanase contributes significantly to resistance against P. opuntiae. Activity of this enzyme and the phytochemical profile can be used as criteria to predict and detect cactus germplasm with resistance to black spot.
Fil: Ochoa, Maria Judith. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: González Flores, L. M.. Instituto Tecnológico de Tlajomulco; México
Fil: Cruz Rubio, J. M.. Instituto Tecnológico de Tlajomulco; México
Fil: Rivera López, L. A.. Instituto Tecnológico de Tlajomulco; México
Fil: Rodriguez, Sergio A.. Universidad Nacional de Santiago del Estero; Argentina
Fil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Gómez Leyva, J. F.. Instituto Tecnológico de Tlajomulco; México
Materia
PATHOGENESIS-RELATED-PROTEINS
CACTUS PEAR
FUNGAL DISEASE
ANTIFUNGAL PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155408
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/155408
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodesOchoa, Maria JudithGonzález Flores, L. M.Cruz Rubio, J. M.Rivera López, L. A.Rodriguez, Sergio A.Nazareno, Mónica AzucenaGómez Leyva, J. F.PATHOGENESIS-RELATED-PROTEINSCACTUS PEARFUNGAL DISEASEANTIFUNGAL PROTEINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Black spot disease, caused by the hemibiotrophic fungus Pseudocercospora opuntiae, is one of the main phytosanitary problems of cactus (Opuntia spp.). Through mass selection, one cultivar of Opuntia ficus-indica (L.) Mill. resistant to colonization by P. opuntiae was identified. The ethanolic extract of resistant cladodes showed higher levels of total condensed tannins, flavonoids and polyphenols than those of the susceptible genotypes, generating 93% inhibition of P. opuntiae conidial germination in vitro. The total protein in the resistant genotype showed 300% higher β-1,3-glucanase than the susceptible genotype. This increased activity was able to inhibit germination of conidia by 90%, a similar effect to that of the fungicide Captan® (N‑trichloromethylthio-4-cyclohexene 1,2-dicarboximide). It was shown, for the first time, that the combined action of cactus polyphenols and β-1,3-glucanase contributes significantly to resistance against P. opuntiae. Activity of this enzyme and the phytochemical profile can be used as criteria to predict and detect cactus germplasm with resistance to black spot.Fil: Ochoa, Maria Judith. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: González Flores, L. M.. Instituto Tecnológico de Tlajomulco; MéxicoFil: Cruz Rubio, J. M.. Instituto Tecnológico de Tlajomulco; MéxicoFil: Rivera López, L. A.. Instituto Tecnológico de Tlajomulco; MéxicoFil: Rodriguez, Sergio A.. Universidad Nacional de Santiago del Estero; ArgentinaFil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Gómez Leyva, J. F.. Instituto Tecnológico de Tlajomulco; MéxicoInternational Society for Horticultural Science2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155408Ochoa, Maria Judith; González Flores, L. M.; Cruz Rubio, J. M. ; Rivera López, L. A. ; Rodriguez, Sergio A.; et al.; Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes; International Society for Horticultural Science; Acta Horticulturae; 25; 8-2019; 183-1900567-7572CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17660/ActaHortic.2019.1247.25info:eu-repo/semantics/altIdentifier/url/https://www.actahort.org/books/1247/1247_25.htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:36Zoai:ri.conicet.gov.ar:11336/155408instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:36.849CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
title Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
spellingShingle Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
Ochoa, Maria Judith
PATHOGENESIS-RELATED-PROTEINS
CACTUS PEAR
FUNGAL DISEASE
ANTIFUNGAL PROTEIN
title_short Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
title_full Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
title_fullStr Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
title_full_unstemmed Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
title_sort Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes
dc.creator.none.fl_str_mv Ochoa, Maria Judith
González Flores, L. M.
Cruz Rubio, J. M.
Rivera López, L. A.
Rodriguez, Sergio A.
Nazareno, Mónica Azucena
Gómez Leyva, J. F.
author Ochoa, Maria Judith
author_facet Ochoa, Maria Judith
González Flores, L. M.
Cruz Rubio, J. M.
Rivera López, L. A.
Rodriguez, Sergio A.
Nazareno, Mónica Azucena
Gómez Leyva, J. F.
author_role author
author2 González Flores, L. M.
Cruz Rubio, J. M.
Rivera López, L. A.
Rodriguez, Sergio A.
Nazareno, Mónica Azucena
Gómez Leyva, J. F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PATHOGENESIS-RELATED-PROTEINS
CACTUS PEAR
FUNGAL DISEASE
ANTIFUNGAL PROTEIN
topic PATHOGENESIS-RELATED-PROTEINS
CACTUS PEAR
FUNGAL DISEASE
ANTIFUNGAL PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Black spot disease, caused by the hemibiotrophic fungus Pseudocercospora opuntiae, is one of the main phytosanitary problems of cactus (Opuntia spp.). Through mass selection, one cultivar of Opuntia ficus-indica (L.) Mill. resistant to colonization by P. opuntiae was identified. The ethanolic extract of resistant cladodes showed higher levels of total condensed tannins, flavonoids and polyphenols than those of the susceptible genotypes, generating 93% inhibition of P. opuntiae conidial germination in vitro. The total protein in the resistant genotype showed 300% higher β-1,3-glucanase than the susceptible genotype. This increased activity was able to inhibit germination of conidia by 90%, a similar effect to that of the fungicide Captan® (N‑trichloromethylthio-4-cyclohexene 1,2-dicarboximide). It was shown, for the first time, that the combined action of cactus polyphenols and β-1,3-glucanase contributes significantly to resistance against P. opuntiae. Activity of this enzyme and the phytochemical profile can be used as criteria to predict and detect cactus germplasm with resistance to black spot.
Fil: Ochoa, Maria Judith. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: González Flores, L. M.. Instituto Tecnológico de Tlajomulco; México
Fil: Cruz Rubio, J. M.. Instituto Tecnológico de Tlajomulco; México
Fil: Rivera López, L. A.. Instituto Tecnológico de Tlajomulco; México
Fil: Rodriguez, Sergio A.. Universidad Nacional de Santiago del Estero; Argentina
Fil: Nazareno, Mónica Azucena. Universidad Nacional de Santiago del Estero; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Gómez Leyva, J. F.. Instituto Tecnológico de Tlajomulco; México
description Black spot disease, caused by the hemibiotrophic fungus Pseudocercospora opuntiae, is one of the main phytosanitary problems of cactus (Opuntia spp.). Through mass selection, one cultivar of Opuntia ficus-indica (L.) Mill. resistant to colonization by P. opuntiae was identified. The ethanolic extract of resistant cladodes showed higher levels of total condensed tannins, flavonoids and polyphenols than those of the susceptible genotypes, generating 93% inhibition of P. opuntiae conidial germination in vitro. The total protein in the resistant genotype showed 300% higher β-1,3-glucanase than the susceptible genotype. This increased activity was able to inhibit germination of conidia by 90%, a similar effect to that of the fungicide Captan® (N‑trichloromethylthio-4-cyclohexene 1,2-dicarboximide). It was shown, for the first time, that the combined action of cactus polyphenols and β-1,3-glucanase contributes significantly to resistance against P. opuntiae. Activity of this enzyme and the phytochemical profile can be used as criteria to predict and detect cactus germplasm with resistance to black spot.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155408
Ochoa, Maria Judith; González Flores, L. M.; Cruz Rubio, J. M. ; Rivera López, L. A. ; Rodriguez, Sergio A.; et al.; Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes; International Society for Horticultural Science; Acta Horticulturae; 25; 8-2019; 183-190
0567-7572
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155408
identifier_str_mv Ochoa, Maria Judith; González Flores, L. M.; Cruz Rubio, J. M. ; Rivera López, L. A. ; Rodriguez, Sergio A.; et al.; Resistance of cactus pear (Opuntia ficus-indica) against Pseudocercospora opuntiae through β‑1,3‑glucanase activity and polyphenolic compounds in cladodes; International Society for Horticultural Science; Acta Horticulturae; 25; 8-2019; 183-190
0567-7572
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.17660/ActaHortic.2019.1247.25
info:eu-repo/semantics/altIdentifier/url/https://www.actahort.org/books/1247/1247_25.htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Society for Horticultural Science
publisher.none.fl_str_mv International Society for Horticultural Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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