First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil

Autores
Conforto, Erica Cinthia; Bernanrdi Lima, Nelson; Garcete-Gómez, J. M.; Câmara, M. P. S.; Michereff, S. J.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608).
Instituto de Patología Vegetal
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Câmara, M. P. S. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Michereff, S. J. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fuente
Plant Disease 100 (6) : 1238 (June 2016)
Materia
Opuntia
Brasil
Opuntia Ficus Indica
Cladodes
Cladodios
Prickly Pear
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9790
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9790
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in BrazilConforto, Erica CinthiaBernanrdi Lima, NelsonGarcete-Gómez, J. M.Câmara, M. P. S.Michereff, S. J.OpuntiaBrasilOpuntia Ficus IndicaCladodesCladodiosPrickly PearCactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608).Instituto de Patología VegetalFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bernardi Lima, Nelson. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; BrasilFil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; BrasilFil: Câmara, M. P. S. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; BrasilFil: Michereff, S. J. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; BrasilAmerican Phytopathological Society2021-07-13T10:29:36Z2021-07-13T10:29:36Z2016-03-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9790https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN0191-29171943-7692 (online)https://doi.org/10.1094/PDIS-11-15-1298-PDNPlant Disease 100 (6) : 1238 (June 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:58Zoai:localhost:20.500.12123/9790instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:58.563INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
title First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
spellingShingle First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
Conforto, Erica Cinthia
Opuntia
Brasil
Opuntia Ficus Indica
Cladodes
Cladodios
Prickly Pear
title_short First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
title_full First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
title_fullStr First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
title_full_unstemmed First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
title_sort First Report of Cladode Brown Spot in Cactus Prickly Pear Caused by Neofusicoccum batangarum in Brazil
dc.creator.none.fl_str_mv Conforto, Erica Cinthia
Bernanrdi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author Conforto, Erica Cinthia
author_facet Conforto, Erica Cinthia
Bernanrdi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author_role author
author2 Bernanrdi Lima, Nelson
Garcete-Gómez, J. M.
Câmara, M. P. S.
Michereff, S. J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Opuntia
Brasil
Opuntia Ficus Indica
Cladodes
Cladodios
Prickly Pear
topic Opuntia
Brasil
Opuntia Ficus Indica
Cladodes
Cladodios
Prickly Pear
dc.description.none.fl_txt_mv Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608).
Instituto de Patología Vegetal
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bernardi Lima, Nelson. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Garcete-Gómez, José María. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Câmara, M. P. S. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
Fil: Michereff, S. J. Universidade Federal Rural de Pernambuco. Departamento de Agronomia; Brasil
description Cactus prickly pear (Nopalea cochenilifera) cladodes showing brown spot symptoms were collected of 18 fields of the State of Pernambuco, northeastern Brazil, from March to June 2014. The symptoms were prevalent in 100% of fields surveyed. Small pieces (4 to 5 mm) of necrotic tissues were surface sterilized for 1 min in 1.5% NaOCl, washed twice with sterile distilled water, and plated onto potato dextrose agar (PDA) amended with 0.5 g /liter streptomycin sulfate. Colonies morphologically similar to species of Botryosphaeriaceae were transferred to malt extract agar (MEA); five isolates (CMM 1424, CMM 1425, CMM 1426, CMM 1427, and CMM 1428) presented colonies forming concentric rings, and white mycelium becoming gray to gray-olivaceous after 5 days. Conidial characters were observed after growth on 2% water agar bearing sterilized pine needles for 3 weeks at 25°C under near-UV light. Conidiogenous cells holoblastic, hyaline, smooth, and cylindrical. Conidia were nonseptate, hyaline, smooth, fusoid to ovoid, thin-walled, 15.3 ± 1.4 × 5.4 ± 0.6 µm (n = 50), L/W ratio= 2.8, which are morphological and cultural characteristics typical of Neofusicoccum spp. (Phillips et al. 2013). DNA sequencing of part of the elongation factor 1-alpha (EF1-α) gene and the internal transcribed spacer (ITS1-5.8S-ITS2 rDNA) region were conducted to identify the species as described by (Marques et al. 2013). Sequences of the isolates were 99% similar to those of N. batangarum for EF1-α (GenBank Accession Nos. FJ900653 and FJ900654) and ITS (FJ900607 and FJ900608).
publishDate 2016
dc.date.none.fl_str_mv 2016-03-18
2021-07-13T10:29:36Z
2021-07-13T10:29:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9790
https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN
0191-2917
1943-7692 (online)
https://doi.org/10.1094/PDIS-11-15-1298-PDN
url http://hdl.handle.net/20.500.12123/9790
https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-15-1298-PDN
https://doi.org/10.1094/PDIS-11-15-1298-PDN
identifier_str_mv 0191-2917
1943-7692 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Plant Disease 100 (6) : 1238 (June 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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