Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements

Autores
Morales Urrea, Diego Alberto; Haure, Patricia Monica; Contreras, Edgardo Martin
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, three xenobiotics (orange II, phenol, and bisphenol A) were oxidized by hydrogen peroxide in the presence of a horseradish peroxidase (HRP) using a fed-batch system. During the experiments, the oxidation-reduction potential (ORP) of the reaction mixture was measured continuously. Results demonstrate that ORP values only increased when both substrates of the enzyme (hydrogen peroxide and the target compound) were present in the reaction mixture. For all of the tested pollutants, the continuous addition of hydrogen peroxide caused an increase in ORP values. When the reducing substrate was depleted, the addition of an excess of hydrogen peroxide caused a decrease of ORP values. The time at which ORP reached a maximum represented the end of the oxidation process. This maximum could be easily detected by means of the derivative of ORP as a function of time. To extend the application of the developed technique, the enzymatic oxidation of a binary mixture of BPA and OII was also followed using ORP measurements. Results were similar to those observed with only one reducing substrate. This work demonstrates that ORP measurements can be useful to maximize hydrogen peroxide efficiency through the controlled addition of the oxidant during the oxidation of OII, phenol, and BPA catalyzed by HRP. This approach allows a minimization of time and process costs since the reaction end-point can be easily detected on a real-time basis.
Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Haure, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
Horseradish Peroxidase
Orange Ii
Bpa
Phenol
Hydrogen Peroxide
Orp
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82181

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network_name_str CONICET Digital (CONICET)
spelling Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurementsMorales Urrea, Diego AlbertoHaure, Patricia MonicaContreras, Edgardo MartinHorseradish PeroxidaseOrange IiBpaPhenolHydrogen PeroxideOrphttps://purl.org/becyt/ford/2.7https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2In this work, three xenobiotics (orange II, phenol, and bisphenol A) were oxidized by hydrogen peroxide in the presence of a horseradish peroxidase (HRP) using a fed-batch system. During the experiments, the oxidation-reduction potential (ORP) of the reaction mixture was measured continuously. Results demonstrate that ORP values only increased when both substrates of the enzyme (hydrogen peroxide and the target compound) were present in the reaction mixture. For all of the tested pollutants, the continuous addition of hydrogen peroxide caused an increase in ORP values. When the reducing substrate was depleted, the addition of an excess of hydrogen peroxide caused a decrease of ORP values. The time at which ORP reached a maximum represented the end of the oxidation process. This maximum could be easily detected by means of the derivative of ORP as a function of time. To extend the application of the developed technique, the enzymatic oxidation of a binary mixture of BPA and OII was also followed using ORP measurements. Results were similar to those observed with only one reducing substrate. This work demonstrates that ORP measurements can be useful to maximize hydrogen peroxide efficiency through the controlled addition of the oxidant during the oxidation of OII, phenol, and BPA catalyzed by HRP. This approach allows a minimization of time and process costs since the reaction end-point can be easily detected on a real-time basis.Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Haure, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaAmerican Chemical Society2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82181Morales Urrea, Diego Alberto; Haure, Patricia Monica; Contreras, Edgardo Martin; Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements; American Chemical Society; Industrial & Engineering Chemical Research; 57; 48; 12-2018; 16518-165250888-5885CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.iecr.8b04616info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.iecr.8b04616info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:05Zoai:ri.conicet.gov.ar:11336/82181instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:06.246CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
title Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
spellingShingle Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
Morales Urrea, Diego Alberto
Horseradish Peroxidase
Orange Ii
Bpa
Phenol
Hydrogen Peroxide
Orp
title_short Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
title_full Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
title_fullStr Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
title_full_unstemmed Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
title_sort Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements
dc.creator.none.fl_str_mv Morales Urrea, Diego Alberto
Haure, Patricia Monica
Contreras, Edgardo Martin
author Morales Urrea, Diego Alberto
author_facet Morales Urrea, Diego Alberto
Haure, Patricia Monica
Contreras, Edgardo Martin
author_role author
author2 Haure, Patricia Monica
Contreras, Edgardo Martin
author2_role author
author
dc.subject.none.fl_str_mv Horseradish Peroxidase
Orange Ii
Bpa
Phenol
Hydrogen Peroxide
Orp
topic Horseradish Peroxidase
Orange Ii
Bpa
Phenol
Hydrogen Peroxide
Orp
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.7
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work, three xenobiotics (orange II, phenol, and bisphenol A) were oxidized by hydrogen peroxide in the presence of a horseradish peroxidase (HRP) using a fed-batch system. During the experiments, the oxidation-reduction potential (ORP) of the reaction mixture was measured continuously. Results demonstrate that ORP values only increased when both substrates of the enzyme (hydrogen peroxide and the target compound) were present in the reaction mixture. For all of the tested pollutants, the continuous addition of hydrogen peroxide caused an increase in ORP values. When the reducing substrate was depleted, the addition of an excess of hydrogen peroxide caused a decrease of ORP values. The time at which ORP reached a maximum represented the end of the oxidation process. This maximum could be easily detected by means of the derivative of ORP as a function of time. To extend the application of the developed technique, the enzymatic oxidation of a binary mixture of BPA and OII was also followed using ORP measurements. Results were similar to those observed with only one reducing substrate. This work demonstrates that ORP measurements can be useful to maximize hydrogen peroxide efficiency through the controlled addition of the oxidant during the oxidation of OII, phenol, and BPA catalyzed by HRP. This approach allows a minimization of time and process costs since the reaction end-point can be easily detected on a real-time basis.
Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Haure, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description In this work, three xenobiotics (orange II, phenol, and bisphenol A) were oxidized by hydrogen peroxide in the presence of a horseradish peroxidase (HRP) using a fed-batch system. During the experiments, the oxidation-reduction potential (ORP) of the reaction mixture was measured continuously. Results demonstrate that ORP values only increased when both substrates of the enzyme (hydrogen peroxide and the target compound) were present in the reaction mixture. For all of the tested pollutants, the continuous addition of hydrogen peroxide caused an increase in ORP values. When the reducing substrate was depleted, the addition of an excess of hydrogen peroxide caused a decrease of ORP values. The time at which ORP reached a maximum represented the end of the oxidation process. This maximum could be easily detected by means of the derivative of ORP as a function of time. To extend the application of the developed technique, the enzymatic oxidation of a binary mixture of BPA and OII was also followed using ORP measurements. Results were similar to those observed with only one reducing substrate. This work demonstrates that ORP measurements can be useful to maximize hydrogen peroxide efficiency through the controlled addition of the oxidant during the oxidation of OII, phenol, and BPA catalyzed by HRP. This approach allows a minimization of time and process costs since the reaction end-point can be easily detected on a real-time basis.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82181
Morales Urrea, Diego Alberto; Haure, Patricia Monica; Contreras, Edgardo Martin; Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements; American Chemical Society; Industrial & Engineering Chemical Research; 57; 48; 12-2018; 16518-16525
0888-5885
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82181
identifier_str_mv Morales Urrea, Diego Alberto; Haure, Patricia Monica; Contreras, Edgardo Martin; Monitoring the enzymatic oxidation of xenobiotics by hydrogen peroxide through oxidation-reduction potential measurements; American Chemical Society; Industrial & Engineering Chemical Research; 57; 48; 12-2018; 16518-16525
0888-5885
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acs.iecr.8b04616
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.iecr.8b04616
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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