Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy

Autores
Marioni, Juliana; Mugas, María Laura; Martinez, Florencia; Romero, Bianca Carolina; Gomez, Tomás Isaac; Lingua, Giuliana; Konigheim, Brenda Salome; Núñez Montoya, Susana Carolina
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BackgroundAntimicrobial Photodynamic Therapy (aPDT) has demonstrated effectiveness against various Candida biofilms, typical resistant to conventional treatments. Some strategies have shown to enhance the photoactivity of some photosensitizers (PS), such as the use of a multiple irradiation scheme or the combination with drugs that improve the penetration of the PS through the microbial membrane.PurposeHaving demonstrated the photodynamic antibiofilm activity of some natural anthraquinones (AQs), we selected rubiadin 1-methyl ether (R-1ME) that showed a low photo-reduction percentage (%R) on the biofilm mass, with the aim to improve its effect.Study DesignExperimental in vitro photo-stimulation protocols have been developed, which include successive light exposures and the combination of this AQ with a commonly used antifungal such as Amphotericin B (AmB).MethodsThe biofilms reduction was quantified by Crystal Violet staining. Reactive oxygen and nitrosative species were observed as action mechanism, alongside an assessment of antioxidant response through superoxide dismutase enzyme activation and total antioxidant system capacity.ResultsApplying R-1ME to C. tropicalis biofilms with sequential 15-min irradiation sessions at varied incubation intervals (0, 3, 6, 24, 27, and 30 h) yielded a substantial photo-reduction (62.9 %R) on biofilm mass, even halving the bioactive concentration of R-1ME. Moreover, combining R-1ME with AmB under this irradiation pattern produced an even greater impact (82 %R) at concentrations below the Minimal Inhibitory Concentration. Evident redox imbalances in the biofilm were linked to this photosensitized activity.ConclusionsA new strategy was found to improve the activity of a natural PS on fungal biofilms, by combining it with antifungal drugs, under a staged irradiation scheme, which, in turn, required low doses of the PS and the antifungal to achieve this improved photo-reduction.
Fil: Marioni, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Mugas, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Martinez, Florencia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Romero, Bianca Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gomez, Tomás Isaac. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lingua, Giuliana. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Konigheim, Brenda Salome. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Núñez Montoya, Susana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
ANTHAQUINONES
PHOTODYNAMIC ACTIVITY
CANDIDA BIOFILMS
MULTIPLE IRRADIATION STEPS
OXIDATIVE STRESS
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
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oai:ri.conicet.gov.ar:11336/240410
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network_name_str CONICET Digital (CONICET)
spelling Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategyMarioni, JulianaMugas, María LauraMartinez, FlorenciaRomero, Bianca CarolinaGomez, Tomás IsaacLingua, GiulianaKonigheim, Brenda SalomeNúñez Montoya, Susana CarolinaANTHAQUINONESPHOTODYNAMIC ACTIVITYCANDIDA BIOFILMSMULTIPLE IRRADIATION STEPSOXIDATIVE STRESShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1BackgroundAntimicrobial Photodynamic Therapy (aPDT) has demonstrated effectiveness against various Candida biofilms, typical resistant to conventional treatments. Some strategies have shown to enhance the photoactivity of some photosensitizers (PS), such as the use of a multiple irradiation scheme or the combination with drugs that improve the penetration of the PS through the microbial membrane.PurposeHaving demonstrated the photodynamic antibiofilm activity of some natural anthraquinones (AQs), we selected rubiadin 1-methyl ether (R-1ME) that showed a low photo-reduction percentage (%R) on the biofilm mass, with the aim to improve its effect.Study DesignExperimental in vitro photo-stimulation protocols have been developed, which include successive light exposures and the combination of this AQ with a commonly used antifungal such as Amphotericin B (AmB).MethodsThe biofilms reduction was quantified by Crystal Violet staining. Reactive oxygen and nitrosative species were observed as action mechanism, alongside an assessment of antioxidant response through superoxide dismutase enzyme activation and total antioxidant system capacity.ResultsApplying R-1ME to C. tropicalis biofilms with sequential 15-min irradiation sessions at varied incubation intervals (0, 3, 6, 24, 27, and 30 h) yielded a substantial photo-reduction (62.9 %R) on biofilm mass, even halving the bioactive concentration of R-1ME. Moreover, combining R-1ME with AmB under this irradiation pattern produced an even greater impact (82 %R) at concentrations below the Minimal Inhibitory Concentration. Evident redox imbalances in the biofilm were linked to this photosensitized activity.ConclusionsA new strategy was found to improve the activity of a natural PS on fungal biofilms, by combining it with antifungal drugs, under a staged irradiation scheme, which, in turn, required low doses of the PS and the antifungal to achieve this improved photo-reduction.Fil: Marioni, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Mugas, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Martinez, Florencia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Romero, Bianca Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gomez, Tomás Isaac. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Lingua, Giuliana. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Konigheim, Brenda Salome. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; ArgentinaFil: Núñez Montoya, Susana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaElsevier2024-02info:eu-repo/date/embargoEnd/2024-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240410Marioni, Juliana; Mugas, María Laura; Martinez, Florencia; Romero, Bianca Carolina; Gomez, Tomás Isaac; et al.; Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy; Elsevier; Phytomedicine Plus; 4; 2; 2-2024; 1-332667-0313CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667031324000174info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phyplu.2024.100539info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:22Zoai:ri.conicet.gov.ar:11336/240410instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:23.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
title Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
spellingShingle Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
Marioni, Juliana
ANTHAQUINONES
PHOTODYNAMIC ACTIVITY
CANDIDA BIOFILMS
MULTIPLE IRRADIATION STEPS
OXIDATIVE STRESS
title_short Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
title_full Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
title_fullStr Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
title_full_unstemmed Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
title_sort Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy
dc.creator.none.fl_str_mv Marioni, Juliana
Mugas, María Laura
Martinez, Florencia
Romero, Bianca Carolina
Gomez, Tomás Isaac
Lingua, Giuliana
Konigheim, Brenda Salome
Núñez Montoya, Susana Carolina
author Marioni, Juliana
author_facet Marioni, Juliana
Mugas, María Laura
Martinez, Florencia
Romero, Bianca Carolina
Gomez, Tomás Isaac
Lingua, Giuliana
Konigheim, Brenda Salome
Núñez Montoya, Susana Carolina
author_role author
author2 Mugas, María Laura
Martinez, Florencia
Romero, Bianca Carolina
Gomez, Tomás Isaac
Lingua, Giuliana
Konigheim, Brenda Salome
Núñez Montoya, Susana Carolina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTHAQUINONES
PHOTODYNAMIC ACTIVITY
CANDIDA BIOFILMS
MULTIPLE IRRADIATION STEPS
OXIDATIVE STRESS
topic ANTHAQUINONES
PHOTODYNAMIC ACTIVITY
CANDIDA BIOFILMS
MULTIPLE IRRADIATION STEPS
OXIDATIVE STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv BackgroundAntimicrobial Photodynamic Therapy (aPDT) has demonstrated effectiveness against various Candida biofilms, typical resistant to conventional treatments. Some strategies have shown to enhance the photoactivity of some photosensitizers (PS), such as the use of a multiple irradiation scheme or the combination with drugs that improve the penetration of the PS through the microbial membrane.PurposeHaving demonstrated the photodynamic antibiofilm activity of some natural anthraquinones (AQs), we selected rubiadin 1-methyl ether (R-1ME) that showed a low photo-reduction percentage (%R) on the biofilm mass, with the aim to improve its effect.Study DesignExperimental in vitro photo-stimulation protocols have been developed, which include successive light exposures and the combination of this AQ with a commonly used antifungal such as Amphotericin B (AmB).MethodsThe biofilms reduction was quantified by Crystal Violet staining. Reactive oxygen and nitrosative species were observed as action mechanism, alongside an assessment of antioxidant response through superoxide dismutase enzyme activation and total antioxidant system capacity.ResultsApplying R-1ME to C. tropicalis biofilms with sequential 15-min irradiation sessions at varied incubation intervals (0, 3, 6, 24, 27, and 30 h) yielded a substantial photo-reduction (62.9 %R) on biofilm mass, even halving the bioactive concentration of R-1ME. Moreover, combining R-1ME with AmB under this irradiation pattern produced an even greater impact (82 %R) at concentrations below the Minimal Inhibitory Concentration. Evident redox imbalances in the biofilm were linked to this photosensitized activity.ConclusionsA new strategy was found to improve the activity of a natural PS on fungal biofilms, by combining it with antifungal drugs, under a staged irradiation scheme, which, in turn, required low doses of the PS and the antifungal to achieve this improved photo-reduction.
Fil: Marioni, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Mugas, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Martinez, Florencia. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Romero, Bianca Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gomez, Tomás Isaac. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Lingua, Giuliana. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Konigheim, Brenda Salome. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Cordoba. Facultad de Medicina. Instituto de Virología; Argentina
Fil: Núñez Montoya, Susana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description BackgroundAntimicrobial Photodynamic Therapy (aPDT) has demonstrated effectiveness against various Candida biofilms, typical resistant to conventional treatments. Some strategies have shown to enhance the photoactivity of some photosensitizers (PS), such as the use of a multiple irradiation scheme or the combination with drugs that improve the penetration of the PS through the microbial membrane.PurposeHaving demonstrated the photodynamic antibiofilm activity of some natural anthraquinones (AQs), we selected rubiadin 1-methyl ether (R-1ME) that showed a low photo-reduction percentage (%R) on the biofilm mass, with the aim to improve its effect.Study DesignExperimental in vitro photo-stimulation protocols have been developed, which include successive light exposures and the combination of this AQ with a commonly used antifungal such as Amphotericin B (AmB).MethodsThe biofilms reduction was quantified by Crystal Violet staining. Reactive oxygen and nitrosative species were observed as action mechanism, alongside an assessment of antioxidant response through superoxide dismutase enzyme activation and total antioxidant system capacity.ResultsApplying R-1ME to C. tropicalis biofilms with sequential 15-min irradiation sessions at varied incubation intervals (0, 3, 6, 24, 27, and 30 h) yielded a substantial photo-reduction (62.9 %R) on biofilm mass, even halving the bioactive concentration of R-1ME. Moreover, combining R-1ME with AmB under this irradiation pattern produced an even greater impact (82 %R) at concentrations below the Minimal Inhibitory Concentration. Evident redox imbalances in the biofilm were linked to this photosensitized activity.ConclusionsA new strategy was found to improve the activity of a natural PS on fungal biofilms, by combining it with antifungal drugs, under a staged irradiation scheme, which, in turn, required low doses of the PS and the antifungal to achieve this improved photo-reduction.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
info:eu-repo/date/embargoEnd/2024-08-22
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240410
Marioni, Juliana; Mugas, María Laura; Martinez, Florencia; Romero, Bianca Carolina; Gomez, Tomás Isaac; et al.; Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy; Elsevier; Phytomedicine Plus; 4; 2; 2-2024; 1-33
2667-0313
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240410
identifier_str_mv Marioni, Juliana; Mugas, María Laura; Martinez, Florencia; Romero, Bianca Carolina; Gomez, Tomás Isaac; et al.; Enhanced photodynamic effect of a natural anthraquinone on Candida tropicalis biofilms using a stepwise irradiation strategy; Elsevier; Phytomedicine Plus; 4; 2; 2-2024; 1-33
2667-0313
CONICET Digital
CONICET
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language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phyplu.2024.100539
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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