A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.

Autores
Aguirre Calvo, Tatiana Rocio; Aguirre Calvo, Daniel; Perullini, Ana Mercedes; Santagapita, Patricio Roman
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present research was to optimize the generation of Ca(II)-alginate beads containing extracts from by-products using a blocking design, with a concomitant evaluation of the microstructural changes of the hydrogel network assessed by SAXS. Two main industrial parameters were used as variables: sodium-alginate and calcium-chloride concentrations. Multiple responses related to the loading efficiency of the bioactive compounds, their activity by two methods (ABTS and FRAP) and a macroscopic property (roundness) were analyzed through the experiment design. The blocking design was efficient compared to a randomized design as confirmed by F-value>1, highlighting the differences among the extracts. Besides, both synthesis variables significantly affected each response, showing the concentration of sodium alginate a higher impact. On the other hand, the extracts increased the interconnectivity of the rods (changing the fractal dimension from ∼1.80 to ∼2.10) due to the presence of trivalent cations since they induce a larger coordination of the network. The compactness and size of the rods and the characteristic size of the dimers were more influenced by the extracts than by the increase of alginate and calcium concentrations. The optimized combination of alginate (1.5% w/v) and calcium (2.5% w/v) achieved two goals: highest loading efficiency and activities of bioactive compounds, and stabilization of the microstructural modulations due to extract changes.
Fil: Aguirre Calvo, Tatiana Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Aguirre Calvo, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
ANTIOXIDANTS
HYDROGELS
RESPONSE SURFACE METHODOLOGY
SAXS
WASTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/170670
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/170670
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.Aguirre Calvo, Tatiana RocioAguirre Calvo, DanielPerullini, Ana MercedesSantagapita, Patricio RomanANTIOXIDANTSHYDROGELSRESPONSE SURFACE METHODOLOGYSAXSWASTEhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1The aim of the present research was to optimize the generation of Ca(II)-alginate beads containing extracts from by-products using a blocking design, with a concomitant evaluation of the microstructural changes of the hydrogel network assessed by SAXS. Two main industrial parameters were used as variables: sodium-alginate and calcium-chloride concentrations. Multiple responses related to the loading efficiency of the bioactive compounds, their activity by two methods (ABTS and FRAP) and a macroscopic property (roundness) were analyzed through the experiment design. The blocking design was efficient compared to a randomized design as confirmed by F-value>1, highlighting the differences among the extracts. Besides, both synthesis variables significantly affected each response, showing the concentration of sodium alginate a higher impact. On the other hand, the extracts increased the interconnectivity of the rods (changing the fractal dimension from ∼1.80 to ∼2.10) due to the presence of trivalent cations since they induce a larger coordination of the network. The compactness and size of the rods and the characteristic size of the dimers were more influenced by the extracts than by the increase of alginate and calcium concentrations. The optimized combination of alginate (1.5% w/v) and calcium (2.5% w/v) achieved two goals: highest loading efficiency and activities of bioactive compounds, and stabilization of the microstructural modulations due to extract changes.Fil: Aguirre Calvo, Tatiana Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Aguirre Calvo, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaElsevier2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/170670Aguirre Calvo, Tatiana Rocio; Aguirre Calvo, Daniel; Perullini, Ana Mercedes; Santagapita, Patricio Roman; A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.; Elsevier; Food Hydrocolloids for Health; 1; 1-2021; 1-92667-0259CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667025921000224info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fhfh.2021.100030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:23Zoai:ri.conicet.gov.ar:11336/170670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:24.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
title A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
spellingShingle A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
Aguirre Calvo, Tatiana Rocio
ANTIOXIDANTS
HYDROGELS
RESPONSE SURFACE METHODOLOGY
SAXS
WASTE
title_short A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
title_full A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
title_fullStr A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
title_full_unstemmed A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
title_sort A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.
dc.creator.none.fl_str_mv Aguirre Calvo, Tatiana Rocio
Aguirre Calvo, Daniel
Perullini, Ana Mercedes
Santagapita, Patricio Roman
author Aguirre Calvo, Tatiana Rocio
author_facet Aguirre Calvo, Tatiana Rocio
Aguirre Calvo, Daniel
Perullini, Ana Mercedes
Santagapita, Patricio Roman
author_role author
author2 Aguirre Calvo, Daniel
Perullini, Ana Mercedes
Santagapita, Patricio Roman
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANTS
HYDROGELS
RESPONSE SURFACE METHODOLOGY
SAXS
WASTE
topic ANTIOXIDANTS
HYDROGELS
RESPONSE SURFACE METHODOLOGY
SAXS
WASTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present research was to optimize the generation of Ca(II)-alginate beads containing extracts from by-products using a blocking design, with a concomitant evaluation of the microstructural changes of the hydrogel network assessed by SAXS. Two main industrial parameters were used as variables: sodium-alginate and calcium-chloride concentrations. Multiple responses related to the loading efficiency of the bioactive compounds, their activity by two methods (ABTS and FRAP) and a macroscopic property (roundness) were analyzed through the experiment design. The blocking design was efficient compared to a randomized design as confirmed by F-value>1, highlighting the differences among the extracts. Besides, both synthesis variables significantly affected each response, showing the concentration of sodium alginate a higher impact. On the other hand, the extracts increased the interconnectivity of the rods (changing the fractal dimension from ∼1.80 to ∼2.10) due to the presence of trivalent cations since they induce a larger coordination of the network. The compactness and size of the rods and the characteristic size of the dimers were more influenced by the extracts than by the increase of alginate and calcium concentrations. The optimized combination of alginate (1.5% w/v) and calcium (2.5% w/v) achieved two goals: highest loading efficiency and activities of bioactive compounds, and stabilization of the microstructural modulations due to extract changes.
Fil: Aguirre Calvo, Tatiana Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Aguirre Calvo, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Santagapita, Patricio Roman. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description The aim of the present research was to optimize the generation of Ca(II)-alginate beads containing extracts from by-products using a blocking design, with a concomitant evaluation of the microstructural changes of the hydrogel network assessed by SAXS. Two main industrial parameters were used as variables: sodium-alginate and calcium-chloride concentrations. Multiple responses related to the loading efficiency of the bioactive compounds, their activity by two methods (ABTS and FRAP) and a macroscopic property (roundness) were analyzed through the experiment design. The blocking design was efficient compared to a randomized design as confirmed by F-value>1, highlighting the differences among the extracts. Besides, both synthesis variables significantly affected each response, showing the concentration of sodium alginate a higher impact. On the other hand, the extracts increased the interconnectivity of the rods (changing the fractal dimension from ∼1.80 to ∼2.10) due to the presence of trivalent cations since they induce a larger coordination of the network. The compactness and size of the rods and the characteristic size of the dimers were more influenced by the extracts than by the increase of alginate and calcium concentrations. The optimized combination of alginate (1.5% w/v) and calcium (2.5% w/v) achieved two goals: highest loading efficiency and activities of bioactive compounds, and stabilization of the microstructural modulations due to extract changes.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/170670
Aguirre Calvo, Tatiana Rocio; Aguirre Calvo, Daniel; Perullini, Ana Mercedes; Santagapita, Patricio Roman; A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.; Elsevier; Food Hydrocolloids for Health; 1; 1-2021; 1-9
2667-0259
CONICET Digital
CONICET
url http://hdl.handle.net/11336/170670
identifier_str_mv Aguirre Calvo, Tatiana Rocio; Aguirre Calvo, Daniel; Perullini, Ana Mercedes; Santagapita, Patricio Roman; A detailed microstructural and multiple responses analysis through blocking design to produce Ca(II)-alginate beads loaded with bioactive compounds extracted from by-products.; Elsevier; Food Hydrocolloids for Health; 1; 1-2021; 1-9
2667-0259
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2667025921000224
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fhfh.2021.100030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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