Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis

Autores
Salas, Ana Lilia; Zampini, Iris Catiana; Maldonado, Luis; Isla, Maria Ines
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 µg DE/mL, 200 and 600 µg DE/mL, respectively) and with MIC values of 800 µg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 µg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 µg DE/mL), antihelmintic (LC50 values 70 and 71 µg DE/mL), anti-candida (400 µg DE/mL) and antibacterial activity against Gram-positive bacteria (200µg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia-type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia-type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant.
Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Maldonado, Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Zuccagnia-Type Propolis
2',4'-Dihydroxychalcone
2',4'-Dihydroxy-3'-Methoxychalcone
Antibacterial Activity
Otitis Externa
Ear Drops
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/51962
Acceder
id CONICETDig_19dd2356a1e94f79af58377fe47580d3
oai_identifier_str oai:ri.conicet.gov.ar:11336/51962
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type PropolisSalas, Ana LiliaZampini, Iris CatianaMaldonado, LuisIsla, Maria InesZuccagnia-Type Propolis2',4'-Dihydroxychalcone2',4'-Dihydroxy-3'-MethoxychalconeAntibacterial ActivityOtitis ExternaEar Dropshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 µg DE/mL, 200 and 600 µg DE/mL, respectively) and with MIC values of 800 µg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 µg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 µg DE/mL), antihelmintic (LC50 values 70 and 71 µg DE/mL), anti-candida (400 µg DE/mL) and antibacterial activity against Gram-positive bacteria (200µg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia-type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia-type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant.Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Maldonado, Luis. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaNatural Products2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/51962Salas, Ana Lilia; Zampini, Iris Catiana; Maldonado, Luis; Isla, Maria Ines; Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis; Natural Products; Natural Product Communications; 13; 2; 11-2017; 167-1701934-578X1555-9475CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1801300214info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:04Zoai:ri.conicet.gov.ar:11336/51962instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:04.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
title Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
spellingShingle Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
Salas, Ana Lilia
Zuccagnia-Type Propolis
2',4'-Dihydroxychalcone
2',4'-Dihydroxy-3'-Methoxychalcone
Antibacterial Activity
Otitis Externa
Ear Drops
title_short Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
title_full Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
title_fullStr Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
title_full_unstemmed Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
title_sort Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis
dc.creator.none.fl_str_mv Salas, Ana Lilia
Zampini, Iris Catiana
Maldonado, Luis
Isla, Maria Ines
author Salas, Ana Lilia
author_facet Salas, Ana Lilia
Zampini, Iris Catiana
Maldonado, Luis
Isla, Maria Ines
author_role author
author2 Zampini, Iris Catiana
Maldonado, Luis
Isla, Maria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Zuccagnia-Type Propolis
2',4'-Dihydroxychalcone
2',4'-Dihydroxy-3'-Methoxychalcone
Antibacterial Activity
Otitis Externa
Ear Drops
topic Zuccagnia-Type Propolis
2',4'-Dihydroxychalcone
2',4'-Dihydroxy-3'-Methoxychalcone
Antibacterial Activity
Otitis Externa
Ear Drops
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 µg DE/mL, 200 and 600 µg DE/mL, respectively) and with MIC values of 800 µg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 µg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 µg DE/mL), antihelmintic (LC50 values 70 and 71 µg DE/mL), anti-candida (400 µg DE/mL) and antibacterial activity against Gram-positive bacteria (200µg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia-type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia-type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant.
Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Maldonado, Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description Propolis with a botanic origin in Zuccagnia punctata Cav. (Fabaceae), a plant species widely distributed in the Argentinean Monte region, was used to produce a standardized propolis extract by biological activity and chemical composition. In this paper the effect on microorganisms isolated from otitis was determined. The extract was active against different Staphylococcus strains with MIC and MBC values (75 and 150 µg DE/mL, 200 and 600 µg DE/mL, respectively) and with MIC values of 800 µg DE/mL against Proteus mirabilis. A pharmaceutical product, ear drops containing this extract as bioactive was developed. Stability of ear drops was evaluated for 6 months, and their biological activity, physical and phytochemical properties were preserved. Ear drops showed inhibitory activity on pro-inflammatory enzymes such as lipoxygenase (IC50 values 90 and 100 µg DE/mL), free radical-scavenging effect (SC50 values 23 and 30 µg DE/mL), antihelmintic (LC50 values 70 and 71 µg DE/mL), anti-candida (400 µg DE/mL) and antibacterial activity against Gram-positive bacteria (200µg DE/mL) at zero time and during six month-storage, respectively. The content of two chalcones, chemical markers of Zuccagnia-type propolis was quantified and its level was maintained while stored at room temperature. Its toxicity was also assayed. Our results are interesting since the extract and ear drops of Zuccagnia-type propolis developed could be promising for use in alternative medicine or phytomedicine as antibacterial, anti-candida, antihelmintic, anti-inflammatory and antioxidant.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/51962
Salas, Ana Lilia; Zampini, Iris Catiana; Maldonado, Luis; Isla, Maria Ines; Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis; Natural Products; Natural Product Communications; 13; 2; 11-2017; 167-170
1934-578X
1555-9475
CONICET Digital
CONICET
url http://hdl.handle.net/11336/51962
identifier_str_mv Salas, Ana Lilia; Zampini, Iris Catiana; Maldonado, Luis; Isla, Maria Ines; Development of a Bioproduct for Medicinal Use with Extracts of Zuccagnia-type Propolis; Natural Products; Natural Product Communications; 13; 2; 11-2017; 167-170
1934-578X
1555-9475
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/abs/10.1177/1934578X1801300214
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614024820424704
score 13.070432

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