Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment

Autores
Taborda Gómez, Claudia Marcela; Lairion, Fabiana Norma; Repetto, Marisa Gabriela; Ettcheto, Miren; Merelli, Amalia Margarita; Lazarowski, Alberto Jorge; Auzmendi, Jerónimo Andrés
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cannabidiol (CBD), a lipophilic cannabinoid compound without psychoactive effects, has emerged as adjuvant of anti‐epileptic drugs (AEDs) in the treatment of refractory epilepsy (RE), decreasing the severity and/or frequency of seizures. CBD is considered a multitarget drug that could act throughout the canonical endocannabinoid receptors (CB1‐CB2) or multiple non‐canonical pathways. Despite the fact that the CBD mechanism in RE is still unknown, experiments carried out in our laboratory showed that CBD has an inhibitory role on P‐glycoprotein excretory function, highly related to RE. Since CB2 is expressed mainly in the immune cells, we hypothesized that CBD treatment could alter the activity of polymorphonuclear neutrophils (PMNs) in a similar way that it does with microglia/macrophages and others circulating leukocytes. In vitro, CBD induced PMN cytoplasmatic vacuolization and proapoptotic nuclear condensation, associated with a significantly decreased viability in a concentration‐dependent manner, while low CBD concentration decreased PMN viability in a time‐dependent manner. At a functional level, CBD reduced the chemotaxis and oxygen consumption of PMNs related with superoxide anion production, while the singlet oxygen level was increased suggesting oxidative stress damage. These results are in line with the well-known CBD anti‐inflammatory effect and support a potential immunosuppressor role on PMNs that could promote an eventual defenseless state during chronic treatment with CBD in RE.
Fil: Taborda Gómez, Claudia Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Repetto, Marisa Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Ettcheto, Miren. Universidad de Barcelona; España
Fil: Merelli, Amalia Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Lazarowski, Alberto Jorge. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Auzmendi, Jerónimo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
CANNABIDIOL
CHEMOTAXIS
OXIDATIVE STRESS
OXYGEN CONSUMPTION
POLYMORPHONUCLEAR NEUTROPHILS (PMNS)
REFRACTORY EPILEPSY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/150342
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatmentTaborda Gómez, Claudia MarcelaLairion, Fabiana NormaRepetto, Marisa GabrielaEttcheto, MirenMerelli, Amalia MargaritaLazarowski, Alberto JorgeAuzmendi, Jerónimo AndrésCANNABIDIOLCHEMOTAXISOXIDATIVE STRESSOXYGEN CONSUMPTIONPOLYMORPHONUCLEAR NEUTROPHILS (PMNS)REFRACTORY EPILEPSYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cannabidiol (CBD), a lipophilic cannabinoid compound without psychoactive effects, has emerged as adjuvant of anti‐epileptic drugs (AEDs) in the treatment of refractory epilepsy (RE), decreasing the severity and/or frequency of seizures. CBD is considered a multitarget drug that could act throughout the canonical endocannabinoid receptors (CB1‐CB2) or multiple non‐canonical pathways. Despite the fact that the CBD mechanism in RE is still unknown, experiments carried out in our laboratory showed that CBD has an inhibitory role on P‐glycoprotein excretory function, highly related to RE. Since CB2 is expressed mainly in the immune cells, we hypothesized that CBD treatment could alter the activity of polymorphonuclear neutrophils (PMNs) in a similar way that it does with microglia/macrophages and others circulating leukocytes. In vitro, CBD induced PMN cytoplasmatic vacuolization and proapoptotic nuclear condensation, associated with a significantly decreased viability in a concentration‐dependent manner, while low CBD concentration decreased PMN viability in a time‐dependent manner. At a functional level, CBD reduced the chemotaxis and oxygen consumption of PMNs related with superoxide anion production, while the singlet oxygen level was increased suggesting oxidative stress damage. These results are in line with the well-known CBD anti‐inflammatory effect and support a potential immunosuppressor role on PMNs that could promote an eventual defenseless state during chronic treatment with CBD in RE.Fil: Taborda Gómez, Claudia Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Repetto, Marisa Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Ettcheto, Miren. Universidad de Barcelona; EspañaFil: Merelli, Amalia Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Lazarowski, Alberto Jorge. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; ArgentinaFil: Auzmendi, Jerónimo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaMolecular Diversity Preservation International2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/150342Taborda Gómez, Claudia Marcela; Lairion, Fabiana Norma; Repetto, Marisa Gabriela; Ettcheto, Miren; Merelli, Amalia Margarita; et al.; Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment; Molecular Diversity Preservation International; Pharmaceuticals; 14; 3; 3-2021; 1-121424-8247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8247/14/3/220info:eu-repo/semantics/altIdentifier/doi/10.3390/ph14030220info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:30Zoai:ri.conicet.gov.ar:11336/150342instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:31.004CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
title Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
spellingShingle Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
Taborda Gómez, Claudia Marcela
CANNABIDIOL
CHEMOTAXIS
OXIDATIVE STRESS
OXYGEN CONSUMPTION
POLYMORPHONUCLEAR NEUTROPHILS (PMNS)
REFRACTORY EPILEPSY
title_short Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
title_full Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
title_fullStr Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
title_full_unstemmed Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
title_sort Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment
dc.creator.none.fl_str_mv Taborda Gómez, Claudia Marcela
Lairion, Fabiana Norma
Repetto, Marisa Gabriela
Ettcheto, Miren
Merelli, Amalia Margarita
Lazarowski, Alberto Jorge
Auzmendi, Jerónimo Andrés
author Taborda Gómez, Claudia Marcela
author_facet Taborda Gómez, Claudia Marcela
Lairion, Fabiana Norma
Repetto, Marisa Gabriela
Ettcheto, Miren
Merelli, Amalia Margarita
Lazarowski, Alberto Jorge
Auzmendi, Jerónimo Andrés
author_role author
author2 Lairion, Fabiana Norma
Repetto, Marisa Gabriela
Ettcheto, Miren
Merelli, Amalia Margarita
Lazarowski, Alberto Jorge
Auzmendi, Jerónimo Andrés
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CANNABIDIOL
CHEMOTAXIS
OXIDATIVE STRESS
OXYGEN CONSUMPTION
POLYMORPHONUCLEAR NEUTROPHILS (PMNS)
REFRACTORY EPILEPSY
topic CANNABIDIOL
CHEMOTAXIS
OXIDATIVE STRESS
OXYGEN CONSUMPTION
POLYMORPHONUCLEAR NEUTROPHILS (PMNS)
REFRACTORY EPILEPSY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cannabidiol (CBD), a lipophilic cannabinoid compound without psychoactive effects, has emerged as adjuvant of anti‐epileptic drugs (AEDs) in the treatment of refractory epilepsy (RE), decreasing the severity and/or frequency of seizures. CBD is considered a multitarget drug that could act throughout the canonical endocannabinoid receptors (CB1‐CB2) or multiple non‐canonical pathways. Despite the fact that the CBD mechanism in RE is still unknown, experiments carried out in our laboratory showed that CBD has an inhibitory role on P‐glycoprotein excretory function, highly related to RE. Since CB2 is expressed mainly in the immune cells, we hypothesized that CBD treatment could alter the activity of polymorphonuclear neutrophils (PMNs) in a similar way that it does with microglia/macrophages and others circulating leukocytes. In vitro, CBD induced PMN cytoplasmatic vacuolization and proapoptotic nuclear condensation, associated with a significantly decreased viability in a concentration‐dependent manner, while low CBD concentration decreased PMN viability in a time‐dependent manner. At a functional level, CBD reduced the chemotaxis and oxygen consumption of PMNs related with superoxide anion production, while the singlet oxygen level was increased suggesting oxidative stress damage. These results are in line with the well-known CBD anti‐inflammatory effect and support a potential immunosuppressor role on PMNs that could promote an eventual defenseless state during chronic treatment with CBD in RE.
Fil: Taborda Gómez, Claudia Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Lairion, Fabiana Norma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Repetto, Marisa Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Ettcheto, Miren. Universidad de Barcelona; España
Fil: Merelli, Amalia Margarita. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Lazarowski, Alberto Jorge. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina
Fil: Auzmendi, Jerónimo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Fisiopatología y Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Cannabidiol (CBD), a lipophilic cannabinoid compound without psychoactive effects, has emerged as adjuvant of anti‐epileptic drugs (AEDs) in the treatment of refractory epilepsy (RE), decreasing the severity and/or frequency of seizures. CBD is considered a multitarget drug that could act throughout the canonical endocannabinoid receptors (CB1‐CB2) or multiple non‐canonical pathways. Despite the fact that the CBD mechanism in RE is still unknown, experiments carried out in our laboratory showed that CBD has an inhibitory role on P‐glycoprotein excretory function, highly related to RE. Since CB2 is expressed mainly in the immune cells, we hypothesized that CBD treatment could alter the activity of polymorphonuclear neutrophils (PMNs) in a similar way that it does with microglia/macrophages and others circulating leukocytes. In vitro, CBD induced PMN cytoplasmatic vacuolization and proapoptotic nuclear condensation, associated with a significantly decreased viability in a concentration‐dependent manner, while low CBD concentration decreased PMN viability in a time‐dependent manner. At a functional level, CBD reduced the chemotaxis and oxygen consumption of PMNs related with superoxide anion production, while the singlet oxygen level was increased suggesting oxidative stress damage. These results are in line with the well-known CBD anti‐inflammatory effect and support a potential immunosuppressor role on PMNs that could promote an eventual defenseless state during chronic treatment with CBD in RE.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/150342
Taborda Gómez, Claudia Marcela; Lairion, Fabiana Norma; Repetto, Marisa Gabriela; Ettcheto, Miren; Merelli, Amalia Margarita; et al.; Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment; Molecular Diversity Preservation International; Pharmaceuticals; 14; 3; 3-2021; 1-12
1424-8247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/150342
identifier_str_mv Taborda Gómez, Claudia Marcela; Lairion, Fabiana Norma; Repetto, Marisa Gabriela; Ettcheto, Miren; Merelli, Amalia Margarita; et al.; Cannabidiol (Cbd) alters the functionality of neutrophils (pmn). implications in the refractory epilepsy treatment; Molecular Diversity Preservation International; Pharmaceuticals; 14; 3; 3-2021; 1-12
1424-8247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-8247/14/3/220
info:eu-repo/semantics/altIdentifier/doi/10.3390/ph14030220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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