Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion

Autores
Viera, Marisa Rosana; del Panno, Maria Teresa
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The accumulation of water and sludge in oil storage tanks leads to microbial contamination. The study of those microbial communities is of particular interest, especially the sulphate reducing microorganisms (SRM) which are the main responsible of the microbiologically influenced corrosion (MIC). Usually, the development of those microorganisms is controlled by the addition of biocides. The objective of this work was to evaluate the effect of a biocide (THPS) on those communities in a batch fractionated assay. Fifty flasks containing sterilized tank water were inoculated with an enrichment culture from that water. A set of 25 flask was treated with THPS (250 ppm) leaving the rest as control. Finally, a volume of oil and two carbon steel coupons were added to all flasks and incubated under anoxic conditions. The population dynamics were analysed by counting of heterotrophic bacteria and SRB for 60 days, observing the reduction of the planktonic SRB and the absence of the sessile SRB after 30 days with THPS. However, a low density of SRB was detected forming biofilm after 60 days. PCR-DGGE, with primers directed to the 16SrDNA of Bacteria and Arqueas, and the gene aps of SRB were used. From the analysis of the gel, differences in the community developed in the presence of THPS were observed. Mass sequencing analysis of 16SrDNA allowed the identification of the predominant genera of the resilient populations. The electrochemical tests together with the morphological analysis performed by SEM of the exposed coupons showed an inhibitory effect of the biofilms.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
XVII International Biodegradation & Biodeterioration Symposium
Manchester
Reino Unido
Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas
Materia
BIOCORROSION
OIL STORAGE
BIOCIDE
BACTERIAL POPULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194663
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194663
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosionViera, Marisa Rosanadel Panno, Maria TeresaBIOCORROSIONOIL STORAGEBIOCIDEBACTERIAL POPULATIONhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The accumulation of water and sludge in oil storage tanks leads to microbial contamination. The study of those microbial communities is of particular interest, especially the sulphate reducing microorganisms (SRM) which are the main responsible of the microbiologically influenced corrosion (MIC). Usually, the development of those microorganisms is controlled by the addition of biocides. The objective of this work was to evaluate the effect of a biocide (THPS) on those communities in a batch fractionated assay. Fifty flasks containing sterilized tank water were inoculated with an enrichment culture from that water. A set of 25 flask was treated with THPS (250 ppm) leaving the rest as control. Finally, a volume of oil and two carbon steel coupons were added to all flasks and incubated under anoxic conditions. The population dynamics were analysed by counting of heterotrophic bacteria and SRB for 60 days, observing the reduction of the planktonic SRB and the absence of the sessile SRB after 30 days with THPS. However, a low density of SRB was detected forming biofilm after 60 days. PCR-DGGE, with primers directed to the 16SrDNA of Bacteria and Arqueas, and the gene aps of SRB were used. From the analysis of the gel, differences in the community developed in the presence of THPS were observed. Mass sequencing analysis of 16SrDNA allowed the identification of the predominant genera of the resilient populations. The electrochemical tests together with the morphological analysis performed by SEM of the exposed coupons showed an inhibitory effect of the biofilms.Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaXVII International Biodegradation & Biodeterioration SymposiumManchesterReino UnidoProvincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de PinturasManchester Metropolitan University2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194663Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion; XVII International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 93-93978-0-9926498-1-4CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:22Zoai:ri.conicet.gov.ar:11336/194663instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:22.307CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
title Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
spellingShingle Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
Viera, Marisa Rosana
BIOCORROSION
OIL STORAGE
BIOCIDE
BACTERIAL POPULATION
title_short Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
title_full Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
title_fullStr Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
title_full_unstemmed Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
title_sort Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion
dc.creator.none.fl_str_mv Viera, Marisa Rosana
del Panno, Maria Teresa
author Viera, Marisa Rosana
author_facet Viera, Marisa Rosana
del Panno, Maria Teresa
author_role author
author2 del Panno, Maria Teresa
author2_role author
dc.subject.none.fl_str_mv BIOCORROSION
OIL STORAGE
BIOCIDE
BACTERIAL POPULATION
topic BIOCORROSION
OIL STORAGE
BIOCIDE
BACTERIAL POPULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The accumulation of water and sludge in oil storage tanks leads to microbial contamination. The study of those microbial communities is of particular interest, especially the sulphate reducing microorganisms (SRM) which are the main responsible of the microbiologically influenced corrosion (MIC). Usually, the development of those microorganisms is controlled by the addition of biocides. The objective of this work was to evaluate the effect of a biocide (THPS) on those communities in a batch fractionated assay. Fifty flasks containing sterilized tank water were inoculated with an enrichment culture from that water. A set of 25 flask was treated with THPS (250 ppm) leaving the rest as control. Finally, a volume of oil and two carbon steel coupons were added to all flasks and incubated under anoxic conditions. The population dynamics were analysed by counting of heterotrophic bacteria and SRB for 60 days, observing the reduction of the planktonic SRB and the absence of the sessile SRB after 30 days with THPS. However, a low density of SRB was detected forming biofilm after 60 days. PCR-DGGE, with primers directed to the 16SrDNA of Bacteria and Arqueas, and the gene aps of SRB were used. From the analysis of the gel, differences in the community developed in the presence of THPS were observed. Mass sequencing analysis of 16SrDNA allowed the identification of the predominant genera of the resilient populations. The electrochemical tests together with the morphological analysis performed by SEM of the exposed coupons showed an inhibitory effect of the biofilms.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: del Panno, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
XVII International Biodegradation & Biodeterioration Symposium
Manchester
Reino Unido
Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas
description The accumulation of water and sludge in oil storage tanks leads to microbial contamination. The study of those microbial communities is of particular interest, especially the sulphate reducing microorganisms (SRM) which are the main responsible of the microbiologically influenced corrosion (MIC). Usually, the development of those microorganisms is controlled by the addition of biocides. The objective of this work was to evaluate the effect of a biocide (THPS) on those communities in a batch fractionated assay. Fifty flasks containing sterilized tank water were inoculated with an enrichment culture from that water. A set of 25 flask was treated with THPS (250 ppm) leaving the rest as control. Finally, a volume of oil and two carbon steel coupons were added to all flasks and incubated under anoxic conditions. The population dynamics were analysed by counting of heterotrophic bacteria and SRB for 60 days, observing the reduction of the planktonic SRB and the absence of the sessile SRB after 30 days with THPS. However, a low density of SRB was detected forming biofilm after 60 days. PCR-DGGE, with primers directed to the 16SrDNA of Bacteria and Arqueas, and the gene aps of SRB were used. From the analysis of the gel, differences in the community developed in the presence of THPS were observed. Mass sequencing analysis of 16SrDNA allowed the identification of the predominant genera of the resilient populations. The electrochemical tests together with the morphological analysis performed by SEM of the exposed coupons showed an inhibitory effect of the biofilms.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194663
Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion; XVII International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 93-93
978-0-9926498-1-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194663
identifier_str_mv Effect of THPS on the structure and diversity of the microbial populations in a process of microbially influenced corrosion; XVII International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 93-93
978-0-9926498-1-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Manchester Metropolitan University
publisher.none.fl_str_mv Manchester Metropolitan University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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