Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)

Autores
Realini, Maria Florencia; Poggio, Lidia; González, Graciela Esther
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Knobs heterochromatic blocks occur in all Zea species with 2n = 20, varying in size and number across maize races and their wild relatives (Kato, Mass. Agric. Exp. Stn. Bull.635: 1-185, 1976; McClintock et al., Colegio de Postgraduados, Chapingo, México, 1981; González & Poggio, Genome 54: 26?32, 2011; Poggio et al., Cytogenet. Genome Res. 109: 259?267, 2005). Variation in DNA content has been proposed to be principally due to differences in the amount of heterochromatin, which is mainly located in distal knobs(Laurie & Bennett, Herediy 55:307-313, 1985; Poggio et al., Ann. Bot. 82:115-117, 1998). These structures can be observed as subtelomeric bands by DAPI-banding, and using Fluorescent In Situ Hybridization (FISH) (González et al., Chrom. Res. 14: 629-635, 2006). Knobs have been described as highly repeated tandem arrays of 180-bp and TR-1 (350 bp)sequences, both repeated in different proportions constituting different knobs (Dennis & Peacock, J. Mol. Evol. 20: 341-350, 1984; Ananiev et al., Proc. Natl. Acad. Sci. 95: 10785-10790, 1998). In this study we present the karyotypic formulae, asymmetry indexes and the position and composition of the knobs of two races of maize from Argentine Northeast(NEA), Tupi Amarillo (VAV 6563) and Rosado (VAV 6565). DAPI-banding and FISH techniques were applied. The plant material has been provided by the Vavilov Lab. of Universidad de Buenos Aires (UBA), and cultivated in the greenhouse of the Facultad deAgronomía, UBA.
Fil: Realini, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Materia
Maize Races
Karyotypical Studies
Northeast Argentine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16049
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)Realini, Maria FlorenciaPoggio, LidiaGonzález, Graciela EstherMaize RacesKaryotypical StudiesNortheast Argentinehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Knobs heterochromatic blocks occur in all Zea species with 2n = 20, varying in size and number across maize races and their wild relatives (Kato, Mass. Agric. Exp. Stn. Bull.635: 1-185, 1976; McClintock et al., Colegio de Postgraduados, Chapingo, México, 1981; González & Poggio, Genome 54: 26?32, 2011; Poggio et al., Cytogenet. Genome Res. 109: 259?267, 2005). Variation in DNA content has been proposed to be principally due to differences in the amount of heterochromatin, which is mainly located in distal knobs(Laurie & Bennett, Herediy 55:307-313, 1985; Poggio et al., Ann. Bot. 82:115-117, 1998). These structures can be observed as subtelomeric bands by DAPI-banding, and using Fluorescent In Situ Hybridization (FISH) (González et al., Chrom. Res. 14: 629-635, 2006). Knobs have been described as highly repeated tandem arrays of 180-bp and TR-1 (350 bp)sequences, both repeated in different proportions constituting different knobs (Dennis & Peacock, J. Mol. Evol. 20: 341-350, 1984; Ananiev et al., Proc. Natl. Acad. Sci. 95: 10785-10790, 1998). In this study we present the karyotypic formulae, asymmetry indexes and the position and composition of the knobs of two races of maize from Argentine Northeast(NEA), Tupi Amarillo (VAV 6563) and Rosado (VAV 6565). DAPI-banding and FISH techniques were applied. The plant material has been provided by the Vavilov Lab. of Universidad de Buenos Aires (UBA), and cultivated in the greenhouse of the Facultad deAgronomía, UBA.Fil: Realini, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaUniversity of Illinois2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16049Realini, Maria Florencia; Poggio, Lidia; González, Graciela Esther; Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH); University of Illinois; Maize Genetics Cooperation Newsletter; 87; 3-2013; 1-61090-4573enginfo:eu-repo/semantics/altIdentifier/url/http://mnl.maizegdb.org/87/pdf/09Realini.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:39Zoai:ri.conicet.gov.ar:11336/16049instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:39.723CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
title Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
spellingShingle Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
Realini, Maria Florencia
Maize Races
Karyotypical Studies
Northeast Argentine
title_short Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
title_full Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
title_fullStr Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
title_full_unstemmed Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
title_sort Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH)
dc.creator.none.fl_str_mv Realini, Maria Florencia
Poggio, Lidia
González, Graciela Esther
author Realini, Maria Florencia
author_facet Realini, Maria Florencia
Poggio, Lidia
González, Graciela Esther
author_role author
author2 Poggio, Lidia
González, Graciela Esther
author2_role author
author
dc.subject.none.fl_str_mv Maize Races
Karyotypical Studies
Northeast Argentine
topic Maize Races
Karyotypical Studies
Northeast Argentine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Knobs heterochromatic blocks occur in all Zea species with 2n = 20, varying in size and number across maize races and their wild relatives (Kato, Mass. Agric. Exp. Stn. Bull.635: 1-185, 1976; McClintock et al., Colegio de Postgraduados, Chapingo, México, 1981; González & Poggio, Genome 54: 26?32, 2011; Poggio et al., Cytogenet. Genome Res. 109: 259?267, 2005). Variation in DNA content has been proposed to be principally due to differences in the amount of heterochromatin, which is mainly located in distal knobs(Laurie & Bennett, Herediy 55:307-313, 1985; Poggio et al., Ann. Bot. 82:115-117, 1998). These structures can be observed as subtelomeric bands by DAPI-banding, and using Fluorescent In Situ Hybridization (FISH) (González et al., Chrom. Res. 14: 629-635, 2006). Knobs have been described as highly repeated tandem arrays of 180-bp and TR-1 (350 bp)sequences, both repeated in different proportions constituting different knobs (Dennis & Peacock, J. Mol. Evol. 20: 341-350, 1984; Ananiev et al., Proc. Natl. Acad. Sci. 95: 10785-10790, 1998). In this study we present the karyotypic formulae, asymmetry indexes and the position and composition of the knobs of two races of maize from Argentine Northeast(NEA), Tupi Amarillo (VAV 6563) and Rosado (VAV 6565). DAPI-banding and FISH techniques were applied. The plant material has been provided by the Vavilov Lab. of Universidad de Buenos Aires (UBA), and cultivated in the greenhouse of the Facultad deAgronomía, UBA.
Fil: Realini, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: González, Graciela Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
description Knobs heterochromatic blocks occur in all Zea species with 2n = 20, varying in size and number across maize races and their wild relatives (Kato, Mass. Agric. Exp. Stn. Bull.635: 1-185, 1976; McClintock et al., Colegio de Postgraduados, Chapingo, México, 1981; González & Poggio, Genome 54: 26?32, 2011; Poggio et al., Cytogenet. Genome Res. 109: 259?267, 2005). Variation in DNA content has been proposed to be principally due to differences in the amount of heterochromatin, which is mainly located in distal knobs(Laurie & Bennett, Herediy 55:307-313, 1985; Poggio et al., Ann. Bot. 82:115-117, 1998). These structures can be observed as subtelomeric bands by DAPI-banding, and using Fluorescent In Situ Hybridization (FISH) (González et al., Chrom. Res. 14: 629-635, 2006). Knobs have been described as highly repeated tandem arrays of 180-bp and TR-1 (350 bp)sequences, both repeated in different proportions constituting different knobs (Dennis & Peacock, J. Mol. Evol. 20: 341-350, 1984; Ananiev et al., Proc. Natl. Acad. Sci. 95: 10785-10790, 1998). In this study we present the karyotypic formulae, asymmetry indexes and the position and composition of the knobs of two races of maize from Argentine Northeast(NEA), Tupi Amarillo (VAV 6563) and Rosado (VAV 6565). DAPI-banding and FISH techniques were applied. The plant material has been provided by the Vavilov Lab. of Universidad de Buenos Aires (UBA), and cultivated in the greenhouse of the Facultad deAgronomía, UBA.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16049
Realini, Maria Florencia; Poggio, Lidia; González, Graciela Esther; Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH); University of Illinois; Maize Genetics Cooperation Newsletter; 87; 3-2013; 1-6
1090-4573
url http://hdl.handle.net/11336/16049
identifier_str_mv Realini, Maria Florencia; Poggio, Lidia; González, Graciela Esther; Karyotypical studies of two maize races from Northeast Argentine (NEA): DAPI-banding and Fluorescent In Situ Hybridization (FISH); University of Illinois; Maize Genetics Cooperation Newsletter; 87; 3-2013; 1-6
1090-4573
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://mnl.maizegdb.org/87/pdf/09Realini.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Illinois
publisher.none.fl_str_mv University of Illinois
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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