Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación

Autores
Berner, Silvia Inés; de Bonis, Cristian; Martinez, Diego; Demarchi, Gianina; Pérez Millán, María Inés; Becu, Damasia; Cristina, Silvia Carolina
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction and objectives: Angiogenesis is an essential process in tumor development. Nevertheless, discrepancies in the angiogenic pattern of pituitary tumors, in terms of hormonal phenotype, size or invasiveness have been found. Our aim was to study the expression of VEGF and FGF2 growth factors, and their importance in the vascularization of pituitary adenomas. We also quantified blood vessels with the endothelial cell markers CD31 and CD34 determining the vascular area, and the proliferation rate through PCNA and Ki67 index. Materials and Methods: We studied 76 pituitary macroadenomas that were surgically resected in the period between 2006 and 2010 from a total of 276 patients with this pathology. Adenomas were classified into prolactinomas (PRL), somatotropinomas (GH), corticotropinomas (ACTH), non-functioning (NF) and plurihormonal (Ph) according to their hormonal secretion. Samples were collected in formalin, embedded in paraffin, and immunohistochemistry was performed from histological sections for endothelial markers CD31 and CD34; and for Ki-67 to study cell proliferation. VEGF, CD31 and PCNA were measured by Western blot. We compared results with normal glands (N=6). Results: VEGF expression levels, found in all of the samples analyzed, were higher in resistant prolactinomas than in other pituitary adenomas. This protein was detected in endothelial cells of blood vessels and in tumor cells cytoplasms and nuclei. Fifty-six percent of samples were positive for FGF2, the other potent angiogenic factor studied, showing cytoplasmatic and extracellular matrix localization. We obtained a strong positive correlation between VEGF and CD31 in tumor samples, but we did not find lineal correlation between PCNA and VEGF, or between Ki-67 and VEGF in the samples studied. The vascular area was higher in normal tissues than in tumors when CD34 was used as endothelial cell marker. Conclussion: The importance of studying angiogenesis in pituitary adenomas lies in the need to find molecular markers that can predict tumor behavior. We could demonstrate the expression of VEGF and FGF2, two potent angiogenic factors, and the existence of linear correlation between VEGF and CD31. Our results are indicative of the existence of angiogenesis in pituitary adenomas; therefore the blockage of angiogenesis might be proposed as an alternative strategy for cases of resistance to standard therapy.
Fil: Berner, Silvia Inés. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; Argentina
Fil: de Bonis, Cristian. Clínica Santa Isabel; Argentina
Fil: Martinez, Diego. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; Argentina
Fil: Demarchi, Gianina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
Fil: Pérez Millán, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cristina, Silvia Carolina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
Materia
ADENOMAS HIPOFISARIOS
ANGIOGENESIS
VEGF
PROLIFERACION CELULAR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7355
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7355
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferaciónMechanisms of Pituitary Tumorigenesis. Study of Angiogenic and Proliferation factorsBerner, Silvia Inésde Bonis, CristianMartinez, DiegoDemarchi, GianinaPérez Millán, María InésBecu, DamasiaCristina, Silvia CarolinaADENOMAS HIPOFISARIOSANGIOGENESISVEGFPROLIFERACION CELULARhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Introduction and objectives: Angiogenesis is an essential process in tumor development. Nevertheless, discrepancies in the angiogenic pattern of pituitary tumors, in terms of hormonal phenotype, size or invasiveness have been found. Our aim was to study the expression of VEGF and FGF2 growth factors, and their importance in the vascularization of pituitary adenomas. We also quantified blood vessels with the endothelial cell markers CD31 and CD34 determining the vascular area, and the proliferation rate through PCNA and Ki67 index. Materials and Methods: We studied 76 pituitary macroadenomas that were surgically resected in the period between 2006 and 2010 from a total of 276 patients with this pathology. Adenomas were classified into prolactinomas (PRL), somatotropinomas (GH), corticotropinomas (ACTH), non-functioning (NF) and plurihormonal (Ph) according to their hormonal secretion. Samples were collected in formalin, embedded in paraffin, and immunohistochemistry was performed from histological sections for endothelial markers CD31 and CD34; and for Ki-67 to study cell proliferation. VEGF, CD31 and PCNA were measured by Western blot. We compared results with normal glands (N=6). Results: VEGF expression levels, found in all of the samples analyzed, were higher in resistant prolactinomas than in other pituitary adenomas. This protein was detected in endothelial cells of blood vessels and in tumor cells cytoplasms and nuclei. Fifty-six percent of samples were positive for FGF2, the other potent angiogenic factor studied, showing cytoplasmatic and extracellular matrix localization. We obtained a strong positive correlation between VEGF and CD31 in tumor samples, but we did not find lineal correlation between PCNA and VEGF, or between Ki-67 and VEGF in the samples studied. The vascular area was higher in normal tissues than in tumors when CD34 was used as endothelial cell marker. Conclussion: The importance of studying angiogenesis in pituitary adenomas lies in the need to find molecular markers that can predict tumor behavior. We could demonstrate the expression of VEGF and FGF2, two potent angiogenic factors, and the existence of linear correlation between VEGF and CD31. Our results are indicative of the existence of angiogenesis in pituitary adenomas; therefore the blockage of angiogenesis might be proposed as an alternative strategy for cases of resistance to standard therapy.Fil: Berner, Silvia Inés. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; ArgentinaFil: de Bonis, Cristian. Clínica Santa Isabel; ArgentinaFil: Martinez, Diego. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; ArgentinaFil: Demarchi, Gianina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; ArgentinaFil: Pérez Millán, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cristina, Silvia Carolina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; ArgentinaSociedad Argentina de Endocrinología y Metabolismo2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/mswordhttp://hdl.handle.net/11336/7355Berner, Silvia Inés; de Bonis, Cristian; Martinez, Diego; Demarchi, Gianina; Pérez Millán, María Inés; et al.; Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación; Sociedad Argentina de Endocrinología y Metabolismo; Revista Argentina de Endocrinologia y Metabolismo; 50; 1; 3-2013; 19-240080-2077spainfo:eu-repo/semantics/altIdentifier/url/http://www.raem.org.ar/numeros/2013-vol50/numero-01/vol50-01-002-eng.htmlinfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-30342013000100002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:36Zoai:ri.conicet.gov.ar:11336/7355instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:36.71CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
Mechanisms of Pituitary Tumorigenesis. Study of Angiogenic and Proliferation factors
title Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
spellingShingle Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
Berner, Silvia Inés
ADENOMAS HIPOFISARIOS
ANGIOGENESIS
VEGF
PROLIFERACION CELULAR
title_short Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
title_full Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
title_fullStr Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
title_full_unstemmed Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
title_sort Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación
dc.creator.none.fl_str_mv Berner, Silvia Inés
de Bonis, Cristian
Martinez, Diego
Demarchi, Gianina
Pérez Millán, María Inés
Becu, Damasia
Cristina, Silvia Carolina
author Berner, Silvia Inés
author_facet Berner, Silvia Inés
de Bonis, Cristian
Martinez, Diego
Demarchi, Gianina
Pérez Millán, María Inés
Becu, Damasia
Cristina, Silvia Carolina
author_role author
author2 de Bonis, Cristian
Martinez, Diego
Demarchi, Gianina
Pérez Millán, María Inés
Becu, Damasia
Cristina, Silvia Carolina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ADENOMAS HIPOFISARIOS
ANGIOGENESIS
VEGF
PROLIFERACION CELULAR
topic ADENOMAS HIPOFISARIOS
ANGIOGENESIS
VEGF
PROLIFERACION CELULAR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction and objectives: Angiogenesis is an essential process in tumor development. Nevertheless, discrepancies in the angiogenic pattern of pituitary tumors, in terms of hormonal phenotype, size or invasiveness have been found. Our aim was to study the expression of VEGF and FGF2 growth factors, and their importance in the vascularization of pituitary adenomas. We also quantified blood vessels with the endothelial cell markers CD31 and CD34 determining the vascular area, and the proliferation rate through PCNA and Ki67 index. Materials and Methods: We studied 76 pituitary macroadenomas that were surgically resected in the period between 2006 and 2010 from a total of 276 patients with this pathology. Adenomas were classified into prolactinomas (PRL), somatotropinomas (GH), corticotropinomas (ACTH), non-functioning (NF) and plurihormonal (Ph) according to their hormonal secretion. Samples were collected in formalin, embedded in paraffin, and immunohistochemistry was performed from histological sections for endothelial markers CD31 and CD34; and for Ki-67 to study cell proliferation. VEGF, CD31 and PCNA were measured by Western blot. We compared results with normal glands (N=6). Results: VEGF expression levels, found in all of the samples analyzed, were higher in resistant prolactinomas than in other pituitary adenomas. This protein was detected in endothelial cells of blood vessels and in tumor cells cytoplasms and nuclei. Fifty-six percent of samples were positive for FGF2, the other potent angiogenic factor studied, showing cytoplasmatic and extracellular matrix localization. We obtained a strong positive correlation between VEGF and CD31 in tumor samples, but we did not find lineal correlation between PCNA and VEGF, or between Ki-67 and VEGF in the samples studied. The vascular area was higher in normal tissues than in tumors when CD34 was used as endothelial cell marker. Conclussion: The importance of studying angiogenesis in pituitary adenomas lies in the need to find molecular markers that can predict tumor behavior. We could demonstrate the expression of VEGF and FGF2, two potent angiogenic factors, and the existence of linear correlation between VEGF and CD31. Our results are indicative of the existence of angiogenesis in pituitary adenomas; therefore the blockage of angiogenesis might be proposed as an alternative strategy for cases of resistance to standard therapy.
Fil: Berner, Silvia Inés. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; Argentina
Fil: de Bonis, Cristian. Clínica Santa Isabel; Argentina
Fil: Martinez, Diego. Hospital Santa Lucía; Argentina. Clínica Santa Isabel; Argentina
Fil: Demarchi, Gianina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
Fil: Pérez Millán, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cristina, Silvia Carolina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
description Introduction and objectives: Angiogenesis is an essential process in tumor development. Nevertheless, discrepancies in the angiogenic pattern of pituitary tumors, in terms of hormonal phenotype, size or invasiveness have been found. Our aim was to study the expression of VEGF and FGF2 growth factors, and their importance in the vascularization of pituitary adenomas. We also quantified blood vessels with the endothelial cell markers CD31 and CD34 determining the vascular area, and the proliferation rate through PCNA and Ki67 index. Materials and Methods: We studied 76 pituitary macroadenomas that were surgically resected in the period between 2006 and 2010 from a total of 276 patients with this pathology. Adenomas were classified into prolactinomas (PRL), somatotropinomas (GH), corticotropinomas (ACTH), non-functioning (NF) and plurihormonal (Ph) according to their hormonal secretion. Samples were collected in formalin, embedded in paraffin, and immunohistochemistry was performed from histological sections for endothelial markers CD31 and CD34; and for Ki-67 to study cell proliferation. VEGF, CD31 and PCNA were measured by Western blot. We compared results with normal glands (N=6). Results: VEGF expression levels, found in all of the samples analyzed, were higher in resistant prolactinomas than in other pituitary adenomas. This protein was detected in endothelial cells of blood vessels and in tumor cells cytoplasms and nuclei. Fifty-six percent of samples were positive for FGF2, the other potent angiogenic factor studied, showing cytoplasmatic and extracellular matrix localization. We obtained a strong positive correlation between VEGF and CD31 in tumor samples, but we did not find lineal correlation between PCNA and VEGF, or between Ki-67 and VEGF in the samples studied. The vascular area was higher in normal tissues than in tumors when CD34 was used as endothelial cell marker. Conclussion: The importance of studying angiogenesis in pituitary adenomas lies in the need to find molecular markers that can predict tumor behavior. We could demonstrate the expression of VEGF and FGF2, two potent angiogenic factors, and the existence of linear correlation between VEGF and CD31. Our results are indicative of the existence of angiogenesis in pituitary adenomas; therefore the blockage of angiogenesis might be proposed as an alternative strategy for cases of resistance to standard therapy.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7355
Berner, Silvia Inés; de Bonis, Cristian; Martinez, Diego; Demarchi, Gianina; Pérez Millán, María Inés; et al.; Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación; Sociedad Argentina de Endocrinología y Metabolismo; Revista Argentina de Endocrinologia y Metabolismo; 50; 1; 3-2013; 19-24
0080-2077
url http://hdl.handle.net/11336/7355
identifier_str_mv Berner, Silvia Inés; de Bonis, Cristian; Martinez, Diego; Demarchi, Gianina; Pérez Millán, María Inés; et al.; Mecanismos de tumorogenesis hipofisaria. Estudio de factores angiogénicos y de proliferación; Sociedad Argentina de Endocrinología y Metabolismo; Revista Argentina de Endocrinologia y Metabolismo; 50; 1; 3-2013; 19-24
0080-2077
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.raem.org.ar/numeros/2013-vol50/numero-01/vol50-01-002-eng.html
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-30342013000100002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/msword
dc.publisher.none.fl_str_mv Sociedad Argentina de Endocrinología y Metabolismo
publisher.none.fl_str_mv Sociedad Argentina de Endocrinología y Metabolismo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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