Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
- Autores
- Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; Maggi, Matías Daniel; Demedio, Jorge
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.
Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Flores, Byron. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Castillo, Gladys. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Rodriguez, Daymara. Universidad de La Habana; Cuba
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Demedio, Jorge. Universidad de La Habana; Cuba - Materia
-
AFRICANIZED BEES
APIS MELLIFERA
NICARAGUA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/211306
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineagesDüttmann, ChristianeFlores, ByronSheleby Elías, JessicaCastillo, GladysRodriguez, DaymaraMaggi, Matías DanielDemedio, JorgeAFRICANIZED BEESAPIS MELLIFERANICARAGUAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; NicaraguaFil: Flores, Byron. Universidad Politécnica de Nicaragua; NicaraguaFil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; NicaraguaFil: Castillo, Gladys. Universidad Politécnica de Nicaragua; NicaraguaFil: Rodriguez, Daymara. Universidad de La Habana; CubaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Demedio, Jorge. Universidad de La Habana; CubaPublic Library of Science2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211306Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267600info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267600info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:51Zoai:ri.conicet.gov.ar:11336/211306instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:51.612CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
title |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
spellingShingle |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages Düttmann, Christiane AFRICANIZED BEES APIS MELLIFERA NICARAGUA |
title_short |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
title_full |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
title_fullStr |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
title_full_unstemmed |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
title_sort |
Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages |
dc.creator.none.fl_str_mv |
Düttmann, Christiane Flores, Byron Sheleby Elías, Jessica Castillo, Gladys Rodriguez, Daymara Maggi, Matías Daniel Demedio, Jorge |
author |
Düttmann, Christiane |
author_facet |
Düttmann, Christiane Flores, Byron Sheleby Elías, Jessica Castillo, Gladys Rodriguez, Daymara Maggi, Matías Daniel Demedio, Jorge |
author_role |
author |
author2 |
Flores, Byron Sheleby Elías, Jessica Castillo, Gladys Rodriguez, Daymara Maggi, Matías Daniel Demedio, Jorge |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
AFRICANIZED BEES APIS MELLIFERA NICARAGUA |
topic |
AFRICANIZED BEES APIS MELLIFERA NICARAGUA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes. Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; Nicaragua Fil: Flores, Byron. Universidad Politécnica de Nicaragua; Nicaragua Fil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; Nicaragua Fil: Castillo, Gladys. Universidad Politécnica de Nicaragua; Nicaragua Fil: Rodriguez, Daymara. Universidad de La Habana; Cuba Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Demedio, Jorge. Universidad de La Habana; Cuba |
description |
Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/211306 Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-19 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/211306 |
identifier_str_mv |
Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-19 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267600 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267600 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
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Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |