Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages

Autores
Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; Maggi, Matías Daniel; Demedio, Jorge
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.
Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Flores, Byron. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Castillo, Gladys. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Rodriguez, Daymara. Universidad de La Habana; Cuba
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Demedio, Jorge. Universidad de La Habana; Cuba
Materia
AFRICANIZED BEES
APIS MELLIFERA
NICARAGUA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211306
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineagesDüttmann, ChristianeFlores, ByronSheleby Elías, JessicaCastillo, GladysRodriguez, DaymaraMaggi, Matías DanielDemedio, JorgeAFRICANIZED BEESAPIS MELLIFERANICARAGUAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; NicaraguaFil: Flores, Byron. Universidad Politécnica de Nicaragua; NicaraguaFil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; NicaraguaFil: Castillo, Gladys. Universidad Politécnica de Nicaragua; NicaraguaFil: Rodriguez, Daymara. Universidad de La Habana; CubaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Demedio, Jorge. Universidad de La Habana; CubaPublic Library of Science2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211306Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267600info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267600info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:00Zoai:ri.conicet.gov.ar:11336/211306instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:00.874CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
title Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
spellingShingle Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
Düttmann, Christiane
AFRICANIZED BEES
APIS MELLIFERA
NICARAGUA
title_short Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
title_full Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
title_fullStr Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
title_full_unstemmed Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
title_sort Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages
dc.creator.none.fl_str_mv Düttmann, Christiane
Flores, Byron
Sheleby Elías, Jessica
Castillo, Gladys
Rodriguez, Daymara
Maggi, Matías Daniel
Demedio, Jorge
author Düttmann, Christiane
author_facet Düttmann, Christiane
Flores, Byron
Sheleby Elías, Jessica
Castillo, Gladys
Rodriguez, Daymara
Maggi, Matías Daniel
Demedio, Jorge
author_role author
author2 Flores, Byron
Sheleby Elías, Jessica
Castillo, Gladys
Rodriguez, Daymara
Maggi, Matías Daniel
Demedio, Jorge
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv AFRICANIZED BEES
APIS MELLIFERA
NICARAGUA
topic AFRICANIZED BEES
APIS MELLIFERA
NICARAGUA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.
Fil: Düttmann, Christiane. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Flores, Byron. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Sheleby Elías, Jessica. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Castillo, Gladys. Universidad Politécnica de Nicaragua; Nicaragua
Fil: Rodriguez, Daymara. Universidad de La Habana; Cuba
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Demedio, Jorge. Universidad de La Habana; Cuba
description Various subspecies of Apis mellifera L. were introduced to Central America since colonization 500 years ago. Hybridization increased with the entrance of the Africanized bee in Nicaragua in 1984. Rustic beekeeping activities and numerous feral swarms define the genetic pattern, reflected in phenotypic heterogeneity and remarkable differences in the behaviour of the bee colonies, especially the nest defence. Due to these facts, the question emerge about the degree of Africanization of honeybee colonies in Nicaragua. In this study, we identified Africanized honeybee colonies based on the single character "mean forewing length"and we corroborated our results by determining mitotypes using mtDNA analysis. Morphometric and genetic approaches were realized in three different geographical zones of Nicaragua and related to beehive characteristics and management. Worker bee samples were taken from the inside of 146 hives from 26 apiaries. Abdominal colour as phenotypic character was the first examination, followed by measurement of 1460 right forewings to determine corresponding probability of Africanization. More than 60% of the beehives showed phenotypic heterogeneity and mean forewing length of 8.74 mm (SD 0.16 mm) indicated a high degree of Africanization. Those results provided a selection of 96 worker bees to perform PCR of two worker bees per hive. For mitochondrial DNA analysis 14 samples from sentinel apiaries were added. Three from 61 beehives presented bees with different mtDNA. Throughout, three mitotypes of the African (A) lineage were detected; one mitotype is still unidentified. Mitotype A1 A. mellifera iberiensis was represented by 88 bees and mitotype A4 A. mellifera scutellata by 21 bees. Phylogenetic analysis confirmed the PCR findings. No associations were found between mitotypes, forewing length, beehive characteristics and management. A high degree of Africanization in A. mellifera colonies represented by two predominating mitotypes from the A lineage, prevail in Neotropical Nicaragua, with mitotype A4 predominating at higher altitudes.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211306
Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-19
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211306
identifier_str_mv Düttmann, Christiane; Flores, Byron; Sheleby Elías, Jessica; Castillo, Gladys; Rodriguez, Daymara; et al.; Africanized honeybee population (Apis mellifera L.) in Nicaragua: Forewing length and mitotype lineages; Public Library of Science; Plos One; 17; 4-2022; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0267600
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0267600
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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