IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model

Autores
Galvez, Marcelo Javier; Giorgi, Gisela; Lencinas, Ileana Beatriz; Crescitelli, Maria Carla; Kenny, Agustina; Martínez, Adriana; Murray, María Gabriela; Prat, María Inés
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Allergic rhinitis (AR) has worldwide distribution with an increasingprevalence every year. Also, allergic rhinitis and chronic rhinosinus-itis are frequently associated with asthma. In our region, Russianthistle (Salsola kali) is a common weed causing pollinosis. The aimof this study was to establish a mouse model of AR based on Salsolakali pollen proteins, and to define the extent that this could have inthe lower airways. BALB/c mice (n = 5/group) were administratedweekly with PBS or S. kali pollen extract through i.p. route and lat-er challenged by nasal instillation of PBS or S. kali pollen respec-tively on days 21, 22 and 23. Bronchoalveolar lavage (BAL) wereobtained. Serum total IgE and S. kali-specific IgE were measuredby ELISA. After sacrifice, the noses and lungs were fixed and par-affin embedded for histological analysis (H&E, toluidine blue and periodic acid-Schiff). After nasal challenge, frequency of sneezingin sensitized group were higher than the control (p<0,001). Gobletcell hyperplasia and eosinophil infiltration was observed in nasal lat-eral mucosa (257±40 in sensitized mice vs. 16±2 in control group,p<0,05). IgE values were increased seven times in sensitized micecompared to control group (p<0,01). Moreover, mice in the sensi-tized group had significantly higher levels of S. kali-specific IgE thanthe control (p<0,05). Sensitization induced moderate to severe in-flammatory infiltration in lungs. Significant increased number of totalinflammatory cells were observed in BAL from sensitized mice com-pared to control group (p<0,05) including eosinophils. Our resultsconfirm an upper airway inflammation associated to an IgE-mediat-ed hypersensitivity reaction induced by the aeroallergen exposure.In addition, associated effects were demonstrated in the lungs, al-though more research needs to be done to clarify this upper-lowerairway interaction. The later could be helpful since AR and asthmarespond to similar treatment strategies.
Fil: Galvez, Marcelo Javier. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lencinas, Ileana Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Crescitelli, Maria Carla. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kenny, Agustina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Martínez, Adriana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Murray, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Prat, María Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina De Fisiología
Materia
INFLAMACIÓN
POLEN
igE
MODELO ANIMAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273567
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/273567
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine modelGalvez, Marcelo JavierGiorgi, GiselaLencinas, Ileana BeatrizCrescitelli, Maria CarlaKenny, AgustinaMartínez, AdrianaMurray, María GabrielaPrat, María InésINFLAMACIÓNPOLENigEMODELO ANIMALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Allergic rhinitis (AR) has worldwide distribution with an increasingprevalence every year. Also, allergic rhinitis and chronic rhinosinus-itis are frequently associated with asthma. In our region, Russianthistle (Salsola kali) is a common weed causing pollinosis. The aimof this study was to establish a mouse model of AR based on Salsolakali pollen proteins, and to define the extent that this could have inthe lower airways. BALB/c mice (n = 5/group) were administratedweekly with PBS or S. kali pollen extract through i.p. route and lat-er challenged by nasal instillation of PBS or S. kali pollen respec-tively on days 21, 22 and 23. Bronchoalveolar lavage (BAL) wereobtained. Serum total IgE and S. kali-specific IgE were measuredby ELISA. After sacrifice, the noses and lungs were fixed and par-affin embedded for histological analysis (H&E, toluidine blue and periodic acid-Schiff). After nasal challenge, frequency of sneezingin sensitized group were higher than the control (p<0,001). Gobletcell hyperplasia and eosinophil infiltration was observed in nasal lat-eral mucosa (257±40 in sensitized mice vs. 16±2 in control group,p<0,05). IgE values were increased seven times in sensitized micecompared to control group (p<0,01). Moreover, mice in the sensi-tized group had significantly higher levels of S. kali-specific IgE thanthe control (p<0,05). Sensitization induced moderate to severe in-flammatory infiltration in lungs. Significant increased number of totalinflammatory cells were observed in BAL from sensitized mice com-pared to control group (p<0,05) including eosinophils. Our resultsconfirm an upper airway inflammation associated to an IgE-mediat-ed hypersensitivity reaction induced by the aeroallergen exposure.In addition, associated effects were demonstrated in the lungs, al-though more research needs to be done to clarify this upper-lowerairway interaction. The later could be helpful since AR and asthmarespond to similar treatment strategies.Fil: Galvez, Marcelo Javier. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; ArgentinaFil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Lencinas, Ileana Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; ArgentinaFil: Crescitelli, Maria Carla. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kenny, Agustina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; ArgentinaFil: Martínez, Adriana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; ArgentinaFil: Murray, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Prat, María Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina De FisiologíaFundación Revista MedicinaAlonso, Daniel Fernando2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273567IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 86-870025-7680CONICET DigitalCONICETenghttps://inmunologia.org.ar/reunion-conjunta-anual-2022/info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/36368022.pdfinfo:eu-repo/semantics/altIdentifier/url/https://inmunologia.org.ar/reunion-conjunta-anual-2022/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:28:04Zoai:ri.conicet.gov.ar:11336/273567instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:28:05.045CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
title IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
spellingShingle IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
Galvez, Marcelo Javier
INFLAMACIÓN
POLEN
igE
MODELO ANIMAL
title_short IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
title_full IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
title_fullStr IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
title_full_unstemmed IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
title_sort IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model
dc.creator.none.fl_str_mv Galvez, Marcelo Javier
Giorgi, Gisela
Lencinas, Ileana Beatriz
Crescitelli, Maria Carla
Kenny, Agustina
Martínez, Adriana
Murray, María Gabriela
Prat, María Inés
author Galvez, Marcelo Javier
author_facet Galvez, Marcelo Javier
Giorgi, Gisela
Lencinas, Ileana Beatriz
Crescitelli, Maria Carla
Kenny, Agustina
Martínez, Adriana
Murray, María Gabriela
Prat, María Inés
author_role author
author2 Giorgi, Gisela
Lencinas, Ileana Beatriz
Crescitelli, Maria Carla
Kenny, Agustina
Martínez, Adriana
Murray, María Gabriela
Prat, María Inés
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Alonso, Daniel Fernando
dc.subject.none.fl_str_mv INFLAMACIÓN
POLEN
igE
MODELO ANIMAL
topic INFLAMACIÓN
POLEN
igE
MODELO ANIMAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Allergic rhinitis (AR) has worldwide distribution with an increasingprevalence every year. Also, allergic rhinitis and chronic rhinosinus-itis are frequently associated with asthma. In our region, Russianthistle (Salsola kali) is a common weed causing pollinosis. The aimof this study was to establish a mouse model of AR based on Salsolakali pollen proteins, and to define the extent that this could have inthe lower airways. BALB/c mice (n = 5/group) were administratedweekly with PBS or S. kali pollen extract through i.p. route and lat-er challenged by nasal instillation of PBS or S. kali pollen respec-tively on days 21, 22 and 23. Bronchoalveolar lavage (BAL) wereobtained. Serum total IgE and S. kali-specific IgE were measuredby ELISA. After sacrifice, the noses and lungs were fixed and par-affin embedded for histological analysis (H&E, toluidine blue and periodic acid-Schiff). After nasal challenge, frequency of sneezingin sensitized group were higher than the control (p<0,001). Gobletcell hyperplasia and eosinophil infiltration was observed in nasal lat-eral mucosa (257±40 in sensitized mice vs. 16±2 in control group,p<0,05). IgE values were increased seven times in sensitized micecompared to control group (p<0,01). Moreover, mice in the sensi-tized group had significantly higher levels of S. kali-specific IgE thanthe control (p<0,05). Sensitization induced moderate to severe in-flammatory infiltration in lungs. Significant increased number of totalinflammatory cells were observed in BAL from sensitized mice com-pared to control group (p<0,05) including eosinophils. Our resultsconfirm an upper airway inflammation associated to an IgE-mediat-ed hypersensitivity reaction induced by the aeroallergen exposure.In addition, associated effects were demonstrated in the lungs, al-though more research needs to be done to clarify this upper-lowerairway interaction. The later could be helpful since AR and asthmarespond to similar treatment strategies.
Fil: Galvez, Marcelo Javier. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Giorgi, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lencinas, Ileana Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Crescitelli, Maria Carla. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kenny, Agustina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Martínez, Adriana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
Fil: Murray, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Prat, María Inés. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Inmunología; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina De Fisiología
description Allergic rhinitis (AR) has worldwide distribution with an increasingprevalence every year. Also, allergic rhinitis and chronic rhinosinus-itis are frequently associated with asthma. In our region, Russianthistle (Salsola kali) is a common weed causing pollinosis. The aimof this study was to establish a mouse model of AR based on Salsolakali pollen proteins, and to define the extent that this could have inthe lower airways. BALB/c mice (n = 5/group) were administratedweekly with PBS or S. kali pollen extract through i.p. route and lat-er challenged by nasal instillation of PBS or S. kali pollen respec-tively on days 21, 22 and 23. Bronchoalveolar lavage (BAL) wereobtained. Serum total IgE and S. kali-specific IgE were measuredby ELISA. After sacrifice, the noses and lungs were fixed and par-affin embedded for histological analysis (H&E, toluidine blue and periodic acid-Schiff). After nasal challenge, frequency of sneezingin sensitized group were higher than the control (p<0,001). Gobletcell hyperplasia and eosinophil infiltration was observed in nasal lat-eral mucosa (257±40 in sensitized mice vs. 16±2 in control group,p<0,05). IgE values were increased seven times in sensitized micecompared to control group (p<0,01). Moreover, mice in the sensi-tized group had significantly higher levels of S. kali-specific IgE thanthe control (p<0,05). Sensitization induced moderate to severe in-flammatory infiltration in lungs. Significant increased number of totalinflammatory cells were observed in BAL from sensitized mice com-pared to control group (p<0,05) including eosinophils. Our resultsconfirm an upper airway inflammation associated to an IgE-mediat-ed hypersensitivity reaction induced by the aeroallergen exposure.In addition, associated effects were demonstrated in the lungs, al-though more research needs to be done to clarify this upper-lowerairway interaction. The later could be helpful since AR and asthmarespond to similar treatment strategies.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273567
IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 86-87
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273567
identifier_str_mv IgE-mediated upper airway inflammation in a russian thistle pollen-induced murine model; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de La Sociedad Argentina de Inmunología; 3er Congreso Franco-Argentino De Inmunología y Reunión Anual 2022 de La Sociedad Argentina De Fisiología; Mar del Plata; Argentina; 2022; 86-87
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://inmunologia.org.ar/reunion-conjunta-anual-2022/
info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/PMID/36368022.pdf
info:eu-repo/semantics/altIdentifier/url/https://inmunologia.org.ar/reunion-conjunta-anual-2022/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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