Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3

Autores
Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots.  In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots.  However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia.  Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs.  We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA.  When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots.  We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain.  Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.
Fil: Lee, Angie. University of California at Los Angeles; Estados Unidos
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Hirsch, Ann M.. University of California at Los Angeles; Estados Unidos
Materia
MAENOD40
CYTOKININ
MELILOTUS ALBA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241668

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network_name_str CONICET Digital (CONICET)
spelling Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3Lee, AngieGiordano, Walter FabianHirsch, Ann M.MAENOD40CYTOKININMELILOTUS ALBAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots.  In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots.  However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia.  Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs.  We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA.  When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots.  We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain.  Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.Fil: Lee, Angie. University of California at Los Angeles; Estados UnidosFil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados UnidosFil: Hirsch, Ann M.. University of California at Los Angeles; Estados UnidosTaylor & Francis2008-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241668Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-2231559-2324CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.3.4.5317info:eu-repo/semantics/altIdentifier/doi/10.4161/psb.3.4.5317info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:31Zoai:ri.conicet.gov.ar:11336/241668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:32.126CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
title Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
spellingShingle Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
Lee, Angie
MAENOD40
CYTOKININ
MELILOTUS ALBA
title_short Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
title_full Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
title_fullStr Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
title_full_unstemmed Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
title_sort Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
dc.creator.none.fl_str_mv Lee, Angie
Giordano, Walter Fabian
Hirsch, Ann M.
author Lee, Angie
author_facet Lee, Angie
Giordano, Walter Fabian
Hirsch, Ann M.
author_role author
author2 Giordano, Walter Fabian
Hirsch, Ann M.
author2_role author
author
dc.subject.none.fl_str_mv MAENOD40
CYTOKININ
MELILOTUS ALBA
topic MAENOD40
CYTOKININ
MELILOTUS ALBA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots.  In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots.  However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia.  Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs.  We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA.  When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots.  We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain.  Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.
Fil: Lee, Angie. University of California at Los Angeles; Estados Unidos
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Hirsch, Ann M.. University of California at Los Angeles; Estados Unidos
description We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots.  In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots.  However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia.  Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs.  We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA.  When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots.  We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain.  Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.
publishDate 2008
dc.date.none.fl_str_mv 2008-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241668
Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-223
1559-2324
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241668
identifier_str_mv Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-223
1559-2324
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.3.4.5317
info:eu-repo/semantics/altIdentifier/doi/10.4161/psb.3.4.5317
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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