Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3
- Autores
- Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots. However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia. Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs. We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA. When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots. We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain. Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.
Fil: Lee, Angie. University of California at Los Angeles; Estados Unidos
Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados Unidos
Fil: Hirsch, Ann M.. University of California at Los Angeles; Estados Unidos - Materia
-
MAENOD40
CYTOKININ
MELILOTUS ALBA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/241668
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network_name_str |
CONICET Digital (CONICET) |
spelling |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3Lee, AngieGiordano, Walter FabianHirsch, Ann M.MAENOD40CYTOKININMELILOTUS ALBAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots. However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia. Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs. We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA. When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots. We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain. Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression.Fil: Lee, Angie. University of California at Los Angeles; Estados UnidosFil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados UnidosFil: Hirsch, Ann M.. University of California at Los Angeles; Estados UnidosTaylor & Francis2008-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241668Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-2231559-2324CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.3.4.5317info:eu-repo/semantics/altIdentifier/doi/10.4161/psb.3.4.5317info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:31Zoai:ri.conicet.gov.ar:11336/241668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:32.126CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
title |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
spellingShingle |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 Lee, Angie MAENOD40 CYTOKININ MELILOTUS ALBA |
title_short |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
title_full |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
title_fullStr |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
title_full_unstemmed |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
title_sort |
Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3 |
dc.creator.none.fl_str_mv |
Lee, Angie Giordano, Walter Fabian Hirsch, Ann M. |
author |
Lee, Angie |
author_facet |
Lee, Angie Giordano, Walter Fabian Hirsch, Ann M. |
author_role |
author |
author2 |
Giordano, Walter Fabian Hirsch, Ann M. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
MAENOD40 CYTOKININ MELILOTUS ALBA |
topic |
MAENOD40 CYTOKININ MELILOTUS ALBA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots. However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia. Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs. We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA. When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots. We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain. Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression. Fil: Lee, Angie. University of California at Los Angeles; Estados Unidos Fil: Giordano, Walter Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. University of California at Los Angeles; Estados Unidos Fil: Hirsch, Ann M.. University of California at Los Angeles; Estados Unidos |
description |
We previously showed that applying 10-6 M of the cytokinin 6-benzylaminopurine (BAP) to uninoculated roots of Masym3, a Nod-Myc- Melilotus alba (Desr.) mutant, resulted in the accumulation of MaENOD40 transcripts to levels similar to BAP-treated wild-type roots. In contrast, inoculation with a Nod- S. meliloti mutant expressing the trans-zeatin synthase gene of Agrobacterium tumefaciens (Nod-/pTZS+) did not induce MaENOD40 transcript accumulation in either wild-type or Masym3 roots. However, Masym3 root hairs became swollen at their tips following inoculation with wild-type or Nod-/pTZS+ rhizobia. Because root hair distention and elongation are often correlated with increased expansin activity, we investigated whether BAP treatment or inoculation with Nod-/pTZS+ S. meliloti up-regulated the expression of expansin mRNAs. We first determined that treating wild-type roots with 10-5 or 10-6 M BAP resulted in greater MaEXPA1 transcript accumulation than treating roots with comparable concentrations of the auxin NAA. When Masym3 roots were treated with 10-6 M BAP, MaEXPA1 mRNAs accumulated to levels comparable to wild-type roots. We then showed that MaEXPA1 mRNAs accumulated in wild-type M. alba roots in response to the Nod-/pTZS+ S. meliloti strain. Masym3 mutant roots inoculated with Nod-/pTZS+ rhizobia were also up-regulated for MaEXPA1 expression. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/241668 Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-223 1559-2324 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/241668 |
identifier_str_mv |
Lee, Angie; Giordano, Walter Fabian; Hirsch, Ann M.; Cytokinin induces expansin gene expression in Melilotus alba Desr. wild-type and the non-nodulating, non-mycorrhizal (Nod - Myc - ) mutant Masym3; Taylor & Francis; Plant Signaling & Behavior; 3; 4; 3-2008; 218-223 1559-2324 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.4161/psb.3.4.5317 info:eu-repo/semantics/altIdentifier/doi/10.4161/psb.3.4.5317 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268978484871168 |
score |
13.13397 |