Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis
- Autores
- Burgardt, Noelia Ines; Melian, Noelia Agostina; Gonzalez Flecha, Francisco Luis
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Copper homeostasis is a key process in organisms, with distinctive pathways that have developed according to specific needs but conserve the main resistance mechanisms. These systems are well described in model bacterium, but there is little information about copper resistance in species adapted to cold environments. In this work we explore the repertoire of genes that might be related to copper homeostasis in the genome of Bizionia argentinensis (JUB59-T), a psychrotolerant bacteria isolated from the Antarctic sea water. Several genes were found encoding proteins similar to well-known proteins essential for copper homeostasis, including three sequences of copper-transport P1B-type ATPases. We selected one of these, denoted as BaCopA1, for cloning and expression in Saccharomyces cerevisiae. BaCopA1 was successfully incorporated into yeasts membranes, from where it was removed with detergent. Purified BaCopA1 catalyzes ATP hydrolysis at low temperature. The structural models of different BaCopA1 conformations were obtained and compared to the mesophilic and thermophilic homologous structures. The high conservation of essential residues and the structural similarity between these proteins suggest that they share the same reaction mechanism for copper transport. This is the first report of a psychrotolerant P1B-ATPase expressed and purified in a functional form.
Fil: Burgardt, Noelia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Melian, Noelia Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina - Materia
-
COPPER REGULATION
ANTARTIC BACTERIA
PSYCHROTOLERANT ENZYMES
COPPER P-ATPASES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/239980
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensisBurgardt, Noelia InesMelian, Noelia AgostinaGonzalez Flecha, Francisco LuisCOPPER REGULATIONANTARTIC BACTERIAPSYCHROTOLERANT ENZYMESCOPPER P-ATPASEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Copper homeostasis is a key process in organisms, with distinctive pathways that have developed according to specific needs but conserve the main resistance mechanisms. These systems are well described in model bacterium, but there is little information about copper resistance in species adapted to cold environments. In this work we explore the repertoire of genes that might be related to copper homeostasis in the genome of Bizionia argentinensis (JUB59-T), a psychrotolerant bacteria isolated from the Antarctic sea water. Several genes were found encoding proteins similar to well-known proteins essential for copper homeostasis, including three sequences of copper-transport P1B-type ATPases. We selected one of these, denoted as BaCopA1, for cloning and expression in Saccharomyces cerevisiae. BaCopA1 was successfully incorporated into yeasts membranes, from where it was removed with detergent. Purified BaCopA1 catalyzes ATP hydrolysis at low temperature. The structural models of different BaCopA1 conformations were obtained and compared to the mesophilic and thermophilic homologous structures. The high conservation of essential residues and the structural similarity between these proteins suggest that they share the same reaction mechanism for copper transport. This is the first report of a psychrotolerant P1B-ATPase expressed and purified in a functional form.Fil: Burgardt, Noelia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Melian, Noelia Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaWiley2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/239980Burgardt, Noelia Ines; Melian, Noelia Agostina; Gonzalez Flecha, Francisco Luis; Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis; Wiley; Environmental Microbiology Reports; 16; 4; 6-2024; 1-161758-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13278info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.13278info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:34Zoai:ri.conicet.gov.ar:11336/239980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:34.299CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
spellingShingle |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis Burgardt, Noelia Ines COPPER REGULATION ANTARTIC BACTERIA PSYCHROTOLERANT ENZYMES COPPER P-ATPASES |
title_short |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_full |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_fullStr |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_full_unstemmed |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
title_sort |
Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis |
dc.creator.none.fl_str_mv |
Burgardt, Noelia Ines Melian, Noelia Agostina Gonzalez Flecha, Francisco Luis |
author |
Burgardt, Noelia Ines |
author_facet |
Burgardt, Noelia Ines Melian, Noelia Agostina Gonzalez Flecha, Francisco Luis |
author_role |
author |
author2 |
Melian, Noelia Agostina Gonzalez Flecha, Francisco Luis |
author2_role |
author author |
dc.subject.none.fl_str_mv |
COPPER REGULATION ANTARTIC BACTERIA PSYCHROTOLERANT ENZYMES COPPER P-ATPASES |
topic |
COPPER REGULATION ANTARTIC BACTERIA PSYCHROTOLERANT ENZYMES COPPER P-ATPASES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Copper homeostasis is a key process in organisms, with distinctive pathways that have developed according to specific needs but conserve the main resistance mechanisms. These systems are well described in model bacterium, but there is little information about copper resistance in species adapted to cold environments. In this work we explore the repertoire of genes that might be related to copper homeostasis in the genome of Bizionia argentinensis (JUB59-T), a psychrotolerant bacteria isolated from the Antarctic sea water. Several genes were found encoding proteins similar to well-known proteins essential for copper homeostasis, including three sequences of copper-transport P1B-type ATPases. We selected one of these, denoted as BaCopA1, for cloning and expression in Saccharomyces cerevisiae. BaCopA1 was successfully incorporated into yeasts membranes, from where it was removed with detergent. Purified BaCopA1 catalyzes ATP hydrolysis at low temperature. The structural models of different BaCopA1 conformations were obtained and compared to the mesophilic and thermophilic homologous structures. The high conservation of essential residues and the structural similarity between these proteins suggest that they share the same reaction mechanism for copper transport. This is the first report of a psychrotolerant P1B-ATPase expressed and purified in a functional form. Fil: Burgardt, Noelia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Melian, Noelia Agostina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Gonzalez Flecha, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina |
description |
Copper homeostasis is a key process in organisms, with distinctive pathways that have developed according to specific needs but conserve the main resistance mechanisms. These systems are well described in model bacterium, but there is little information about copper resistance in species adapted to cold environments. In this work we explore the repertoire of genes that might be related to copper homeostasis in the genome of Bizionia argentinensis (JUB59-T), a psychrotolerant bacteria isolated from the Antarctic sea water. Several genes were found encoding proteins similar to well-known proteins essential for copper homeostasis, including three sequences of copper-transport P1B-type ATPases. We selected one of these, denoted as BaCopA1, for cloning and expression in Saccharomyces cerevisiae. BaCopA1 was successfully incorporated into yeasts membranes, from where it was removed with detergent. Purified BaCopA1 catalyzes ATP hydrolysis at low temperature. The structural models of different BaCopA1 conformations were obtained and compared to the mesophilic and thermophilic homologous structures. The high conservation of essential residues and the structural similarity between these proteins suggest that they share the same reaction mechanism for copper transport. This is the first report of a psychrotolerant P1B-ATPase expressed and purified in a functional form. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/239980 Burgardt, Noelia Ines; Melian, Noelia Agostina; Gonzalez Flecha, Francisco Luis; Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis; Wiley; Environmental Microbiology Reports; 16; 4; 6-2024; 1-16 1758-2229 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/239980 |
identifier_str_mv |
Burgardt, Noelia Ines; Melian, Noelia Agostina; Gonzalez Flecha, Francisco Luis; Copper resistance in the cold: Genome analysis and characterisation of a P IB‐1 ATPase in Bizionia argentinensis; Wiley; Environmental Microbiology Reports; 16; 4; 6-2024; 1-16 1758-2229 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1758-2229.13278 info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.13278 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |