Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation

Autores
Morales Polanco, Fabian; Bates, Christian; Lui, Jennifer; Casson, Joseph; Solari, Clara Andrea; Pizzinga, Mariavittoria; Forte, Gabriela; Griffin, Claire; Garner, Kirsten E. L.; Burt, Harriet E.; Dixon, Hannah L.; Hubbard, Simon; Portela, Paula; Ashe, Mark P.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway.
Fil: Morales Polanco, Fabian. University of Manchester; Reino Unido
Fil: Bates, Christian. University of Manchester; Reino Unido
Fil: Lui, Jennifer. University of Manchester; Reino Unido
Fil: Casson, Joseph. University of Manchester; Reino Unido
Fil: Solari, Clara Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pizzinga, Mariavittoria. University of Manchester; Reino Unido
Fil: Forte, Gabriela. University of Manchester; Reino Unido
Fil: Griffin, Claire. University of Manchester; Reino Unido
Fil: Garner, Kirsten E. L.. University of Manchester; Reino Unido
Fil: Burt, Harriet E.. University of Manchester; Reino Unido
Fil: Dixon, Hannah L.. University of Manchester; Reino Unido
Fil: Hubbard, Simon. University of Manchester; Reino Unido
Fil: Portela, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Ashe, Mark P.. University of Manchester; Reino Unido
Materia
CELL BIOLOGY
MOLECULAR BIOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173323
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/173323
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentationMorales Polanco, FabianBates, ChristianLui, JenniferCasson, JosephSolari, Clara AndreaPizzinga, MariavittoriaForte, GabrielaGriffin, ClaireGarner, Kirsten E. L.Burt, Harriet E.Dixon, Hannah L.Hubbard, SimonPortela, PaulaAshe, Mark P.CELL BIOLOGYMOLECULAR BIOLOGYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway.Fil: Morales Polanco, Fabian. University of Manchester; Reino UnidoFil: Bates, Christian. University of Manchester; Reino UnidoFil: Lui, Jennifer. University of Manchester; Reino UnidoFil: Casson, Joseph. University of Manchester; Reino UnidoFil: Solari, Clara Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pizzinga, Mariavittoria. University of Manchester; Reino UnidoFil: Forte, Gabriela. University of Manchester; Reino UnidoFil: Griffin, Claire. University of Manchester; Reino UnidoFil: Garner, Kirsten E. L.. University of Manchester; Reino UnidoFil: Burt, Harriet E.. University of Manchester; Reino UnidoFil: Dixon, Hannah L.. University of Manchester; Reino UnidoFil: Hubbard, Simon. University of Manchester; Reino UnidoFil: Portela, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ashe, Mark P.. University of Manchester; Reino UnidoCell Press2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173323Morales Polanco, Fabian; Bates, Christian; Lui, Jennifer; Casson, Joseph; Solari, Clara Andrea; et al.; Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation; Cell Press; iScience; 24; 2; 1-2021; 1-362589-0042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2589004221000377info:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2021.102069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:27Zoai:ri.conicet.gov.ar:11336/173323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:27.606CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
title Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
spellingShingle Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
Morales Polanco, Fabian
CELL BIOLOGY
MOLECULAR BIOLOGY
title_short Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
title_full Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
title_fullStr Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
title_full_unstemmed Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
title_sort Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
dc.creator.none.fl_str_mv Morales Polanco, Fabian
Bates, Christian
Lui, Jennifer
Casson, Joseph
Solari, Clara Andrea
Pizzinga, Mariavittoria
Forte, Gabriela
Griffin, Claire
Garner, Kirsten E. L.
Burt, Harriet E.
Dixon, Hannah L.
Hubbard, Simon
Portela, Paula
Ashe, Mark P.
author Morales Polanco, Fabian
author_facet Morales Polanco, Fabian
Bates, Christian
Lui, Jennifer
Casson, Joseph
Solari, Clara Andrea
Pizzinga, Mariavittoria
Forte, Gabriela
Griffin, Claire
Garner, Kirsten E. L.
Burt, Harriet E.
Dixon, Hannah L.
Hubbard, Simon
Portela, Paula
Ashe, Mark P.
author_role author
author2 Bates, Christian
Lui, Jennifer
Casson, Joseph
Solari, Clara Andrea
Pizzinga, Mariavittoria
Forte, Gabriela
Griffin, Claire
Garner, Kirsten E. L.
Burt, Harriet E.
Dixon, Hannah L.
Hubbard, Simon
Portela, Paula
Ashe, Mark P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELL BIOLOGY
MOLECULAR BIOLOGY
topic CELL BIOLOGY
MOLECULAR BIOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway.
Fil: Morales Polanco, Fabian. University of Manchester; Reino Unido
Fil: Bates, Christian. University of Manchester; Reino Unido
Fil: Lui, Jennifer. University of Manchester; Reino Unido
Fil: Casson, Joseph. University of Manchester; Reino Unido
Fil: Solari, Clara Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pizzinga, Mariavittoria. University of Manchester; Reino Unido
Fil: Forte, Gabriela. University of Manchester; Reino Unido
Fil: Griffin, Claire. University of Manchester; Reino Unido
Fil: Garner, Kirsten E. L.. University of Manchester; Reino Unido
Fil: Burt, Harriet E.. University of Manchester; Reino Unido
Fil: Dixon, Hannah L.. University of Manchester; Reino Unido
Fil: Hubbard, Simon. University of Manchester; Reino Unido
Fil: Portela, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Ashe, Mark P.. University of Manchester; Reino Unido
description Glycolysis is a fundamental metabolic pathway for glucose catabolism across biology, and glycolytic enzymes are among the most abundant proteins in cells. Their expression at such levels provides a particular challenge. Here we demonstrate that the glycolytic mRNAs are localized to granules in yeast and human cells. Detailed live cell and smFISH studies in yeast show that the mRNAs are actively translated in granules, and this translation appears critical for the localization. Furthermore, this arrangement is likely to facilitate the higher level organization and control of the glycolytic pathway. Indeed, the degree of fermentation required by cells is intrinsically connected to the extent of mRNA localization to granules. On this basis, we term these granules, core fermentation (CoFe) granules; they appear to represent translation factories, allowing high-level coordinated enzyme synthesis for a critical metabolic pathway.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173323
Morales Polanco, Fabian; Bates, Christian; Lui, Jennifer; Casson, Joseph; Solari, Clara Andrea; et al.; Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation; Cell Press; iScience; 24; 2; 1-2021; 1-36
2589-0042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173323
identifier_str_mv Morales Polanco, Fabian; Bates, Christian; Lui, Jennifer; Casson, Joseph; Solari, Clara Andrea; et al.; Core Fermentation (CoFe) granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation; Cell Press; iScience; 24; 2; 1-2021; 1-36
2589-0042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2589004221000377
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2021.102069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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