Translation fidelity coevolves with longevity

Autores
Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; Zhang, Zhengdong D.; Gladyshev, Vadim N.; Seluanov, Andrei; Gorbunova, Vera
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.
Fil: Ke, Zhonghe. University of Rochester; Estados Unidos
Fil: Mallik, Pramit. University of Rochester; Estados Unidos
Fil: Johnson, Adam B.. University of Rochester; Estados Unidos
Fil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Nevo, Eviatar. University of Haifa; Israel
Fil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados Unidos
Fil: Gladyshev, Vadim N.. Harvard Medical School; Estados Unidos
Fil: Seluanov, Andrei. University of Rochester; Estados Unidos
Fil: Gorbunova, Vera. University of Rochester; Estados Unidos
Materia
AGING
COMPARATIVE BIOLOGY
LONGEVITY
TRANSLATION FIDELITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64707

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spelling Translation fidelity coevolves with longevityKe, ZhongheMallik, PramitJohnson, Adam B.Luna, FacundoNevo, EviatarZhang, Zhengdong D.Gladyshev, Vadim N.Seluanov, AndreiGorbunova, VeraAGINGCOMPARATIVE BIOLOGYLONGEVITYTRANSLATION FIDELITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.Fil: Ke, Zhonghe. University of Rochester; Estados UnidosFil: Mallik, Pramit. University of Rochester; Estados UnidosFil: Johnson, Adam B.. University of Rochester; Estados UnidosFil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Nevo, Eviatar. University of Haifa; IsraelFil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados UnidosFil: Gladyshev, Vadim N.. Harvard Medical School; Estados UnidosFil: Seluanov, Andrei. University of Rochester; Estados UnidosFil: Gorbunova, Vera. University of Rochester; Estados UnidosWiley Blackwell Publishing, Inc2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64707Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-9931474-97181474-9726CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/acel.12628info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/acel.12628info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:28Zoai:ri.conicet.gov.ar:11336/64707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:28.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Translation fidelity coevolves with longevity
title Translation fidelity coevolves with longevity
spellingShingle Translation fidelity coevolves with longevity
Ke, Zhonghe
AGING
COMPARATIVE BIOLOGY
LONGEVITY
TRANSLATION FIDELITY
title_short Translation fidelity coevolves with longevity
title_full Translation fidelity coevolves with longevity
title_fullStr Translation fidelity coevolves with longevity
title_full_unstemmed Translation fidelity coevolves with longevity
title_sort Translation fidelity coevolves with longevity
dc.creator.none.fl_str_mv Ke, Zhonghe
Mallik, Pramit
Johnson, Adam B.
Luna, Facundo
Nevo, Eviatar
Zhang, Zhengdong D.
Gladyshev, Vadim N.
Seluanov, Andrei
Gorbunova, Vera
author Ke, Zhonghe
author_facet Ke, Zhonghe
Mallik, Pramit
Johnson, Adam B.
Luna, Facundo
Nevo, Eviatar
Zhang, Zhengdong D.
Gladyshev, Vadim N.
Seluanov, Andrei
Gorbunova, Vera
author_role author
author2 Mallik, Pramit
Johnson, Adam B.
Luna, Facundo
Nevo, Eviatar
Zhang, Zhengdong D.
Gladyshev, Vadim N.
Seluanov, Andrei
Gorbunova, Vera
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGING
COMPARATIVE BIOLOGY
LONGEVITY
TRANSLATION FIDELITY
topic AGING
COMPARATIVE BIOLOGY
LONGEVITY
TRANSLATION FIDELITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.
Fil: Ke, Zhonghe. University of Rochester; Estados Unidos
Fil: Mallik, Pramit. University of Rochester; Estados Unidos
Fil: Johnson, Adam B.. University of Rochester; Estados Unidos
Fil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Nevo, Eviatar. University of Haifa; Israel
Fil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados Unidos
Fil: Gladyshev, Vadim N.. Harvard Medical School; Estados Unidos
Fil: Seluanov, Andrei. University of Rochester; Estados Unidos
Fil: Gorbunova, Vera. University of Rochester; Estados Unidos
description Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64707
Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-993
1474-9718
1474-9726
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64707
identifier_str_mv Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-993
1474-9718
1474-9726
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/acel.12628
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/acel.12628
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
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