Translation fidelity coevolves with longevity
- Autores
- Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; Zhang, Zhengdong D.; Gladyshev, Vadim N.; Seluanov, Andrei; Gorbunova, Vera
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.
Fil: Ke, Zhonghe. University of Rochester; Estados Unidos
Fil: Mallik, Pramit. University of Rochester; Estados Unidos
Fil: Johnson, Adam B.. University of Rochester; Estados Unidos
Fil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Nevo, Eviatar. University of Haifa; Israel
Fil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados Unidos
Fil: Gladyshev, Vadim N.. Harvard Medical School; Estados Unidos
Fil: Seluanov, Andrei. University of Rochester; Estados Unidos
Fil: Gorbunova, Vera. University of Rochester; Estados Unidos - Materia
-
AGING
COMPARATIVE BIOLOGY
LONGEVITY
TRANSLATION FIDELITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/64707
Ver los metadatos del registro completo
id |
CONICETDig_1027c02ec519be0bbdb3e8752d95f6bc |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/64707 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Translation fidelity coevolves with longevityKe, ZhongheMallik, PramitJohnson, Adam B.Luna, FacundoNevo, EviatarZhang, Zhengdong D.Gladyshev, Vadim N.Seluanov, AndreiGorbunova, VeraAGINGCOMPARATIVE BIOLOGYLONGEVITYTRANSLATION FIDELITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals.Fil: Ke, Zhonghe. University of Rochester; Estados UnidosFil: Mallik, Pramit. University of Rochester; Estados UnidosFil: Johnson, Adam B.. University of Rochester; Estados UnidosFil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Nevo, Eviatar. University of Haifa; IsraelFil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados UnidosFil: Gladyshev, Vadim N.. Harvard Medical School; Estados UnidosFil: Seluanov, Andrei. University of Rochester; Estados UnidosFil: Gorbunova, Vera. University of Rochester; Estados UnidosWiley Blackwell Publishing, Inc2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64707Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-9931474-97181474-9726CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/acel.12628info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/acel.12628info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:28Zoai:ri.conicet.gov.ar:11336/64707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:28.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Translation fidelity coevolves with longevity |
title |
Translation fidelity coevolves with longevity |
spellingShingle |
Translation fidelity coevolves with longevity Ke, Zhonghe AGING COMPARATIVE BIOLOGY LONGEVITY TRANSLATION FIDELITY |
title_short |
Translation fidelity coevolves with longevity |
title_full |
Translation fidelity coevolves with longevity |
title_fullStr |
Translation fidelity coevolves with longevity |
title_full_unstemmed |
Translation fidelity coevolves with longevity |
title_sort |
Translation fidelity coevolves with longevity |
dc.creator.none.fl_str_mv |
Ke, Zhonghe Mallik, Pramit Johnson, Adam B. Luna, Facundo Nevo, Eviatar Zhang, Zhengdong D. Gladyshev, Vadim N. Seluanov, Andrei Gorbunova, Vera |
author |
Ke, Zhonghe |
author_facet |
Ke, Zhonghe Mallik, Pramit Johnson, Adam B. Luna, Facundo Nevo, Eviatar Zhang, Zhengdong D. Gladyshev, Vadim N. Seluanov, Andrei Gorbunova, Vera |
author_role |
author |
author2 |
Mallik, Pramit Johnson, Adam B. Luna, Facundo Nevo, Eviatar Zhang, Zhengdong D. Gladyshev, Vadim N. Seluanov, Andrei Gorbunova, Vera |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
AGING COMPARATIVE BIOLOGY LONGEVITY TRANSLATION FIDELITY |
topic |
AGING COMPARATIVE BIOLOGY LONGEVITY TRANSLATION FIDELITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals. Fil: Ke, Zhonghe. University of Rochester; Estados Unidos Fil: Mallik, Pramit. University of Rochester; Estados Unidos Fil: Johnson, Adam B.. University of Rochester; Estados Unidos Fil: Luna, Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Nevo, Eviatar. University of Haifa; Israel Fil: Zhang, Zhengdong D.. Albert Einstein College of Medicine; Estados Unidos Fil: Gladyshev, Vadim N.. Harvard Medical School; Estados Unidos Fil: Seluanov, Andrei. University of Rochester; Estados Unidos Fil: Gorbunova, Vera. University of Rochester; Estados Unidos |
description |
Whether errors in protein synthesis play a role in aging has been a subject of intense debate. It has been suggested that rare mistakes in protein synthesis in young organisms may result in errors in the protein synthesis machinery, eventually leading to an increasing cascade of errors as organisms age. Studies that followed generally failed to identify a dramatic increase in translation errors with aging. However, whether translation fidelity plays a role in aging remained an open question. To address this issue, we examined the relationship between translation fidelity and maximum lifespan across 17 rodent species with diverse lifespans. To measure translation fidelity, we utilized sensitive luciferase-based reporter constructs with mutations in an amino acid residue critical to luciferase activity, wherein misincorporation of amino acids at this mutated codon re-activated the luciferase. The frequency of amino acid misincorporation at the first and second codon positions showed strong negative correlation with maximum lifespan. This correlation remained significant after phylogenetic correction, indicating that translation fidelity coevolves with longevity. These results give new life to the role of protein synthesis errors in aging: Although the error rate may not significantly change with age, the basal rate of translation errors is important in defining lifespan across mammals. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/64707 Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-993 1474-9718 1474-9726 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/64707 |
identifier_str_mv |
Ke, Zhonghe; Mallik, Pramit; Johnson, Adam B.; Luna, Facundo; Nevo, Eviatar; et al.; Translation fidelity coevolves with longevity; Wiley Blackwell Publishing, Inc; Aging Cell; 16; 5; 10-2017; 988-993 1474-9718 1474-9726 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1111/acel.12628 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/acel.12628 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614052024680448 |
score |
13.070432 |