Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels

Autores
Silva, Mauro Gastón; Falcoff, Nora L.; Corradi, Gerardo Raul; Di Camillo, Norma Alicia; Seguel, Rolando F.; Tabaj, Gabriela C.; Guman, Gabriela R.; de Matteo, Elena Noemí; Nuñez, Myriam; Gironacci, Mariela Mercedes
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Angiotensin-converting enzyme 2 (ACE2) is the receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes COVID-19. Viral entry requires ACE2 and transmembrane protease serine 2 (TMPRSS2). Transcriptomic studies showed that children display lower ACE2 than adults, though gene expression levels do not always correlate with protein levels. We investigated the effect of age on ACE2 and TMPRSS2 protein expression in alveolar type II (AT2) cells in the lungs of children compared to adults. We also analysed the ratio of Ang-(1–7) to Ang II as a surrogate marker of ACE2 activity in the subjects’ lung parenchyma. Methods: Ang II and Ang-(1–7) levels and ACE2 and TMPRSS2 protein expression were measured by radioimmunoassay and immunohistochemistry, respectively. Results: The amount of ACE2-expressing AT2 cells and ACE2 protein content were lower in children than in adults. Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cells. Children presented lower Ang-(1–7)/Ang II ratio than adult suggesting lower ACE2 activity in children. TMPRSS2 protein expression was not influenced by age. Conclusions: These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection. Category of study: Clinical original research Impact: Children display lower ACE2 protein content and activity compared to adults.Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cellsTMPRSS2 protein expression was not influenced by age.These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection.
Fil: Silva, Mauro Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Falcoff, Nora L.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Di Camillo, Norma Alicia. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Seguel, Rolando F.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Tabaj, Gabriela C.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Guman, Gabriela R.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Centro de Estudios Históricos "Profesor Carlos S. A. Segreti". Instituto de Estudios Históricos - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Estudios Históricos; Argentina
Fil: Nuñez, Myriam. Universidad de Buenos Aires; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
Angiotensin-converting enzyme 2 (ACE2)
Effect of age
SARS-CoV-2
COVID-19
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214748
Acceder
id CONICETDig_1613a058422561406aaacfa4ee131c64
oai_identifier_str oai:ri.conicet.gov.ar:11336/214748
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levelsSilva, Mauro GastónFalcoff, Nora L.Corradi, Gerardo RaulDi Camillo, Norma AliciaSeguel, Rolando F.Tabaj, Gabriela C.Guman, Gabriela R.de Matteo, Elena NoemíNuñez, MyriamGironacci, Mariela MercedesAngiotensin-converting enzyme 2 (ACE2)Effect of ageSARS-CoV-2COVID-19https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Angiotensin-converting enzyme 2 (ACE2) is the receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes COVID-19. Viral entry requires ACE2 and transmembrane protease serine 2 (TMPRSS2). Transcriptomic studies showed that children display lower ACE2 than adults, though gene expression levels do not always correlate with protein levels. We investigated the effect of age on ACE2 and TMPRSS2 protein expression in alveolar type II (AT2) cells in the lungs of children compared to adults. We also analysed the ratio of Ang-(1–7) to Ang II as a surrogate marker of ACE2 activity in the subjects’ lung parenchyma. Methods: Ang II and Ang-(1–7) levels and ACE2 and TMPRSS2 protein expression were measured by radioimmunoassay and immunohistochemistry, respectively. Results: The amount of ACE2-expressing AT2 cells and ACE2 protein content were lower in children than in adults. Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cells. Children presented lower Ang-(1–7)/Ang II ratio than adult suggesting lower ACE2 activity in children. TMPRSS2 protein expression was not influenced by age. Conclusions: These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection. Category of study: Clinical original research Impact: Children display lower ACE2 protein content and activity compared to adults.Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cellsTMPRSS2 protein expression was not influenced by age.These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection.Fil: Silva, Mauro Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Falcoff, Nora L.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Di Camillo, Norma Alicia. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Seguel, Rolando F.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Tabaj, Gabriela C.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Guman, Gabriela R.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Centro de Estudios Históricos "Profesor Carlos S. A. Segreti". Instituto de Estudios Históricos - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Estudios Históricos; ArgentinaFil: Nuñez, Myriam. Universidad de Buenos Aires; ArgentinaFil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaInternational Pediatric Research Foundation2022-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214748Silva, Mauro Gastón; Falcoff, Nora L.; Corradi, Gerardo Raul; Di Camillo, Norma Alicia; Seguel, Rolando F.; et al.; Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels; International Pediatric Research Foundation; Pediatric Research; 93; 4; 6-2022; 948-9520031-3998CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41390-022-02163-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:38:55Zoai:ri.conicet.gov.ar:11336/214748instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:38:55.619CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
title Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
spellingShingle Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
Silva, Mauro Gastón
Angiotensin-converting enzyme 2 (ACE2)
Effect of age
SARS-CoV-2
COVID-19
title_short Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
title_full Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
title_fullStr Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
title_full_unstemmed Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
title_sort Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels
dc.creator.none.fl_str_mv Silva, Mauro Gastón
Falcoff, Nora L.
Corradi, Gerardo Raul
Di Camillo, Norma Alicia
Seguel, Rolando F.
Tabaj, Gabriela C.
Guman, Gabriela R.
de Matteo, Elena Noemí
Nuñez, Myriam
Gironacci, Mariela Mercedes
author Silva, Mauro Gastón
author_facet Silva, Mauro Gastón
Falcoff, Nora L.
Corradi, Gerardo Raul
Di Camillo, Norma Alicia
Seguel, Rolando F.
Tabaj, Gabriela C.
Guman, Gabriela R.
de Matteo, Elena Noemí
Nuñez, Myriam
Gironacci, Mariela Mercedes
author_role author
author2 Falcoff, Nora L.
Corradi, Gerardo Raul
Di Camillo, Norma Alicia
Seguel, Rolando F.
Tabaj, Gabriela C.
Guman, Gabriela R.
de Matteo, Elena Noemí
Nuñez, Myriam
Gironacci, Mariela Mercedes
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Angiotensin-converting enzyme 2 (ACE2)
Effect of age
SARS-CoV-2
COVID-19
topic Angiotensin-converting enzyme 2 (ACE2)
Effect of age
SARS-CoV-2
COVID-19
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Angiotensin-converting enzyme 2 (ACE2) is the receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes COVID-19. Viral entry requires ACE2 and transmembrane protease serine 2 (TMPRSS2). Transcriptomic studies showed that children display lower ACE2 than adults, though gene expression levels do not always correlate with protein levels. We investigated the effect of age on ACE2 and TMPRSS2 protein expression in alveolar type II (AT2) cells in the lungs of children compared to adults. We also analysed the ratio of Ang-(1–7) to Ang II as a surrogate marker of ACE2 activity in the subjects’ lung parenchyma. Methods: Ang II and Ang-(1–7) levels and ACE2 and TMPRSS2 protein expression were measured by radioimmunoassay and immunohistochemistry, respectively. Results: The amount of ACE2-expressing AT2 cells and ACE2 protein content were lower in children than in adults. Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cells. Children presented lower Ang-(1–7)/Ang II ratio than adult suggesting lower ACE2 activity in children. TMPRSS2 protein expression was not influenced by age. Conclusions: These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection. Category of study: Clinical original research Impact: Children display lower ACE2 protein content and activity compared to adults.Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cellsTMPRSS2 protein expression was not influenced by age.These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection.
Fil: Silva, Mauro Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Falcoff, Nora L.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Di Camillo, Norma Alicia. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Seguel, Rolando F.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Tabaj, Gabriela C.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Guman, Gabriela R.. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo Gutiérrez"; Argentina. Centro de Estudios Históricos "Profesor Carlos S. A. Segreti". Instituto de Estudios Históricos - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Estudios Históricos; Argentina
Fil: Nuñez, Myriam. Universidad de Buenos Aires; Argentina
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Background: Angiotensin-converting enzyme 2 (ACE2) is the receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which causes COVID-19. Viral entry requires ACE2 and transmembrane protease serine 2 (TMPRSS2). Transcriptomic studies showed that children display lower ACE2 than adults, though gene expression levels do not always correlate with protein levels. We investigated the effect of age on ACE2 and TMPRSS2 protein expression in alveolar type II (AT2) cells in the lungs of children compared to adults. We also analysed the ratio of Ang-(1–7) to Ang II as a surrogate marker of ACE2 activity in the subjects’ lung parenchyma. Methods: Ang II and Ang-(1–7) levels and ACE2 and TMPRSS2 protein expression were measured by radioimmunoassay and immunohistochemistry, respectively. Results: The amount of ACE2-expressing AT2 cells and ACE2 protein content were lower in children than in adults. Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cells. Children presented lower Ang-(1–7)/Ang II ratio than adult suggesting lower ACE2 activity in children. TMPRSS2 protein expression was not influenced by age. Conclusions: These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection. Category of study: Clinical original research Impact: Children display lower ACE2 protein content and activity compared to adults.Ang II levels were higher in children compared to adults and inversely correlated with the amount of ACE2-expressing AT2 cellsTMPRSS2 protein expression was not influenced by age.These results expand on previous transcriptomic studies and may partially explain the low susceptibility of children to SARS-CoV-2 infection.
publishDate 2022
dc.date.none.fl_str_mv 2022-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214748
Silva, Mauro Gastón; Falcoff, Nora L.; Corradi, Gerardo Raul; Di Camillo, Norma Alicia; Seguel, Rolando F.; et al.; Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels; International Pediatric Research Foundation; Pediatric Research; 93; 4; 6-2022; 948-952
0031-3998
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214748
identifier_str_mv Silva, Mauro Gastón; Falcoff, Nora L.; Corradi, Gerardo Raul; Di Camillo, Norma Alicia; Seguel, Rolando F.; et al.; Effect of age on human ACE2 and ACE2-expressing alveolar type II cells levels; International Pediatric Research Foundation; Pediatric Research; 93; 4; 6-2022; 948-952
0031-3998
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41390-022-02163-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Pediatric Research Foundation
publisher.none.fl_str_mv International Pediatric Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1852335286874275840
score 12.952241

Publicaciones similares