Characterization of the enzymatic resistance to β-lactamics in enterobacteria

Autores
Castillo, Natalia Alejandra; Jure, M. A.; Allori, C.; Castillo, M.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Detecting the resistance to CTG in severe infections-by enterobacteria guides to successful treatments. The main resistance mechanisms are the production of extended spectrum β-lactamases (BLEE), chromosomal inducible or derepressed β-lactamases Amp-C type or plasmidic Amp-C. The aim of this study was to investigate and characterize the mechanisms involved in the resistance to CTG in clinical isolations of enterobacteria, by phenotypic and genotypic methods. 64 isolations resistant to CTG were studied (K. pneumoniae, E. coli, Enterobacter spp and Proteus spp ). Antimicrobial agents sensibility and phenotypic detection of Amp-C and BLEE enzymes was performed by diffusion and dilution methods. In cefotaxime resistant E. coli and K. pneumoniae strains a possible enzymatic resistance mechanism was detected by Masuda Bioassay. The genes bla CTX-M-2 and bla PER-2 were detected by PCR. Of 64 isolations, 8 strains were presumptively producers of derepressed Amp-C and 56 were BLEE producers (with prevalence of CTX-M-2). Accompanying resistance was observed. Masuda Bioassay was negative and accompanying resistance suggests it is due an impermeability phenomenon. Our results confirm that the resistance to CTG in our strains is mainly caused by enzymes coded in plasmids so that it merit to establish epidemic control measures and to emphasize wise use of β-lactamic agents available.
Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Jure, M. A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Allori, C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tucumán
Argentina
Asociación de Biología de Tucumán
Materia
ANTIMICROBIAL RESISTANCE
BLEE
ENTEROBACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195010
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of the enzymatic resistance to β-lactamics in enterobacteriaCastillo, Natalia AlejandraJure, M. A.Allori, C.Castillo, M.ANTIMICROBIAL RESISTANCEBLEEENTEROBACTERIAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Detecting the resistance to CTG in severe infections-by enterobacteria guides to successful treatments. The main resistance mechanisms are the production of extended spectrum β-lactamases (BLEE), chromosomal inducible or derepressed β-lactamases Amp-C type or plasmidic Amp-C. The aim of this study was to investigate and characterize the mechanisms involved in the resistance to CTG in clinical isolations of enterobacteria, by phenotypic and genotypic methods. 64 isolations resistant to CTG were studied (K. pneumoniae, E. coli, Enterobacter spp and Proteus spp ). Antimicrobial agents sensibility and phenotypic detection of Amp-C and BLEE enzymes was performed by diffusion and dilution methods. In cefotaxime resistant E. coli and K. pneumoniae strains a possible enzymatic resistance mechanism was detected by Masuda Bioassay. The genes bla CTX-M-2 and bla PER-2 were detected by PCR. Of 64 isolations, 8 strains were presumptively producers of derepressed Amp-C and 56 were BLEE producers (with prevalence of CTX-M-2). Accompanying resistance was observed. Masuda Bioassay was negative and accompanying resistance suggests it is due an impermeability phenomenon. Our results confirm that the resistance to CTG in our strains is mainly caused by enzymes coded in plasmids so that it merit to establish epidemic control measures and to emphasize wise use of β-lactamic agents available.Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Jure, M. A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Allori, C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaXXIII Jornadas Científicas de la Asociación de Biología de TucumánTucumánArgentinaAsociación de Biología de TucumánTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195010Characterization of the enzymatic resistance to β-lactamics in enterobacteria; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tucumán; Argentina; 2006; 310-3100327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v31nSuppl.S/38012Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:11Zoai:ri.conicet.gov.ar:11336/195010instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:13.005CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of the enzymatic resistance to β-lactamics in enterobacteria
title Characterization of the enzymatic resistance to β-lactamics in enterobacteria
spellingShingle Characterization of the enzymatic resistance to β-lactamics in enterobacteria
Castillo, Natalia Alejandra
ANTIMICROBIAL RESISTANCE
BLEE
ENTEROBACTERIA
title_short Characterization of the enzymatic resistance to β-lactamics in enterobacteria
title_full Characterization of the enzymatic resistance to β-lactamics in enterobacteria
title_fullStr Characterization of the enzymatic resistance to β-lactamics in enterobacteria
title_full_unstemmed Characterization of the enzymatic resistance to β-lactamics in enterobacteria
title_sort Characterization of the enzymatic resistance to β-lactamics in enterobacteria
dc.creator.none.fl_str_mv Castillo, Natalia Alejandra
Jure, M. A.
Allori, C.
Castillo, M.
author Castillo, Natalia Alejandra
author_facet Castillo, Natalia Alejandra
Jure, M. A.
Allori, C.
Castillo, M.
author_role author
author2 Jure, M. A.
Allori, C.
Castillo, M.
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE
BLEE
ENTEROBACTERIA
topic ANTIMICROBIAL RESISTANCE
BLEE
ENTEROBACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Detecting the resistance to CTG in severe infections-by enterobacteria guides to successful treatments. The main resistance mechanisms are the production of extended spectrum β-lactamases (BLEE), chromosomal inducible or derepressed β-lactamases Amp-C type or plasmidic Amp-C. The aim of this study was to investigate and characterize the mechanisms involved in the resistance to CTG in clinical isolations of enterobacteria, by phenotypic and genotypic methods. 64 isolations resistant to CTG were studied (K. pneumoniae, E. coli, Enterobacter spp and Proteus spp ). Antimicrobial agents sensibility and phenotypic detection of Amp-C and BLEE enzymes was performed by diffusion and dilution methods. In cefotaxime resistant E. coli and K. pneumoniae strains a possible enzymatic resistance mechanism was detected by Masuda Bioassay. The genes bla CTX-M-2 and bla PER-2 were detected by PCR. Of 64 isolations, 8 strains were presumptively producers of derepressed Amp-C and 56 were BLEE producers (with prevalence of CTX-M-2). Accompanying resistance was observed. Masuda Bioassay was negative and accompanying resistance suggests it is due an impermeability phenomenon. Our results confirm that the resistance to CTG in our strains is mainly caused by enzymes coded in plasmids so that it merit to establish epidemic control measures and to emphasize wise use of β-lactamic agents available.
Fil: Castillo, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Jure, M. A.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Allori, C.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Castillo, M.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
XXIII Jornadas Científicas de la Asociación de Biología de Tucumán
Tucumán
Argentina
Asociación de Biología de Tucumán
description Detecting the resistance to CTG in severe infections-by enterobacteria guides to successful treatments. The main resistance mechanisms are the production of extended spectrum β-lactamases (BLEE), chromosomal inducible or derepressed β-lactamases Amp-C type or plasmidic Amp-C. The aim of this study was to investigate and characterize the mechanisms involved in the resistance to CTG in clinical isolations of enterobacteria, by phenotypic and genotypic methods. 64 isolations resistant to CTG were studied (K. pneumoniae, E. coli, Enterobacter spp and Proteus spp ). Antimicrobial agents sensibility and phenotypic detection of Amp-C and BLEE enzymes was performed by diffusion and dilution methods. In cefotaxime resistant E. coli and K. pneumoniae strains a possible enzymatic resistance mechanism was detected by Masuda Bioassay. The genes bla CTX-M-2 and bla PER-2 were detected by PCR. Of 64 isolations, 8 strains were presumptively producers of derepressed Amp-C and 56 were BLEE producers (with prevalence of CTX-M-2). Accompanying resistance was observed. Masuda Bioassay was negative and accompanying resistance suggests it is due an impermeability phenomenon. Our results confirm that the resistance to CTG in our strains is mainly caused by enzymes coded in plasmids so that it merit to establish epidemic control measures and to emphasize wise use of β-lactamic agents available.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195010
Characterization of the enzymatic resistance to β-lactamics in enterobacteria; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tucumán; Argentina; 2006; 310-310
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195010
identifier_str_mv Characterization of the enzymatic resistance to β-lactamics in enterobacteria; XXIII Jornadas Científicas de la Asociación de Biología de Tucumán; Tucumán; Argentina; 2006; 310-310
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://techscience.com/biocell/v31nSuppl.S/38012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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