Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes

Autores
Theumer, Martín Gustavo; Clop, Pedro Diego; Rubinstein, Héctor Ramón; Perillo, Maria Angelica
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we investigate the effects of FB1 at the molecular and mesoscopic levels in FB1–phospholipid mixed Langmuir films. Mean molecular area vs FB1 molar fractions (xFB1) and phase diagram analysis allowed us to define miscibility conditions and phase states at different xFB1. Surface potential measurements, evaluated as a function of the molecular packing and xFB1, revealed the FB1-induced change in the collective dipolar reorientation leaded to neutralization of charged films. Size, shape, and distribution of 2D-domain analysis from epifluorescence data suggested the increase in the mixing entropy and film relaxation rate. Finally, PM-IRRAS revealed the orientation of FB1 with the amine end (zwitterionic and negatively charged monolayers) or the tricarballylic acid end (positively charged monolayers) pointing to the air. The globular-extended conformational equilibrium of FB1 is dynamically defined by the membrane charge becoming a toxicity enhancing factor. The specificity of the toxin–protein interaction might also be perturbed by the FB1-induced remodeling of the membrane topography by affecting the raft-like platforms where membrane enzymes are considered to be located.
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rubinstein, Héctor Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
MYCOTOXINS
FUMONISIN B1
EPIFLUORESCENCE MICROSCOPY
LANGMUIR FILMSPM-IRRAS.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267970
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267970
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model MembranesTheumer, Martín GustavoClop, Pedro DiegoRubinstein, Héctor RamónPerillo, Maria AngelicaMYCOTOXINSFUMONISIN B1EPIFLUORESCENCE MICROSCOPYLANGMUIR FILMSPM-IRRAS.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we investigate the effects of FB1 at the molecular and mesoscopic levels in FB1–phospholipid mixed Langmuir films. Mean molecular area vs FB1 molar fractions (xFB1) and phase diagram analysis allowed us to define miscibility conditions and phase states at different xFB1. Surface potential measurements, evaluated as a function of the molecular packing and xFB1, revealed the FB1-induced change in the collective dipolar reorientation leaded to neutralization of charged films. Size, shape, and distribution of 2D-domain analysis from epifluorescence data suggested the increase in the mixing entropy and film relaxation rate. Finally, PM-IRRAS revealed the orientation of FB1 with the amine end (zwitterionic and negatively charged monolayers) or the tricarballylic acid end (positively charged monolayers) pointing to the air. The globular-extended conformational equilibrium of FB1 is dynamically defined by the membrane charge becoming a toxicity enhancing factor. The specificity of the toxin–protein interaction might also be perturbed by the FB1-induced remodeling of the membrane topography by affecting the raft-like platforms where membrane enzymes are considered to be located.Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Rubinstein, Héctor Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaAmerican Chemical Society2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267970Theumer, Martín Gustavo; Clop, Pedro Diego; Rubinstein, Héctor Ramón; Perillo, Maria Angelica; Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes; American Chemical Society; Journal of Physical Chemistry B; 116; 49; 12-2012; 14216-142271520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jp306350kinfo:eu-repo/semantics/altIdentifier/doi/10.1021/jp306350kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:05Zoai:ri.conicet.gov.ar:11336/267970instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:05.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
title Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
spellingShingle Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
Theumer, Martín Gustavo
MYCOTOXINS
FUMONISIN B1
EPIFLUORESCENCE MICROSCOPY
LANGMUIR FILMSPM-IRRAS.
title_short Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
title_full Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
title_fullStr Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
title_full_unstemmed Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
title_sort Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes
dc.creator.none.fl_str_mv Theumer, Martín Gustavo
Clop, Pedro Diego
Rubinstein, Héctor Ramón
Perillo, Maria Angelica
author Theumer, Martín Gustavo
author_facet Theumer, Martín Gustavo
Clop, Pedro Diego
Rubinstein, Héctor Ramón
Perillo, Maria Angelica
author_role author
author2 Clop, Pedro Diego
Rubinstein, Héctor Ramón
Perillo, Maria Angelica
author2_role author
author
author
dc.subject.none.fl_str_mv MYCOTOXINS
FUMONISIN B1
EPIFLUORESCENCE MICROSCOPY
LANGMUIR FILMSPM-IRRAS.
topic MYCOTOXINS
FUMONISIN B1
EPIFLUORESCENCE MICROSCOPY
LANGMUIR FILMSPM-IRRAS.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we investigate the effects of FB1 at the molecular and mesoscopic levels in FB1–phospholipid mixed Langmuir films. Mean molecular area vs FB1 molar fractions (xFB1) and phase diagram analysis allowed us to define miscibility conditions and phase states at different xFB1. Surface potential measurements, evaluated as a function of the molecular packing and xFB1, revealed the FB1-induced change in the collective dipolar reorientation leaded to neutralization of charged films. Size, shape, and distribution of 2D-domain analysis from epifluorescence data suggested the increase in the mixing entropy and film relaxation rate. Finally, PM-IRRAS revealed the orientation of FB1 with the amine end (zwitterionic and negatively charged monolayers) or the tricarballylic acid end (positively charged monolayers) pointing to the air. The globular-extended conformational equilibrium of FB1 is dynamically defined by the membrane charge becoming a toxicity enhancing factor. The specificity of the toxin–protein interaction might also be perturbed by the FB1-induced remodeling of the membrane topography by affecting the raft-like platforms where membrane enzymes are considered to be located.
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Clop, Pedro Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rubinstein, Héctor Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description The toxicity of FB1 is usually explained through the enzymatic disruption of lipidic metabolism. However, it may lie in the thermodynamics of the membrane and its cooperative phase behavior rather than in the activity of individual proteins. Here, we investigate the effects of FB1 at the molecular and mesoscopic levels in FB1–phospholipid mixed Langmuir films. Mean molecular area vs FB1 molar fractions (xFB1) and phase diagram analysis allowed us to define miscibility conditions and phase states at different xFB1. Surface potential measurements, evaluated as a function of the molecular packing and xFB1, revealed the FB1-induced change in the collective dipolar reorientation leaded to neutralization of charged films. Size, shape, and distribution of 2D-domain analysis from epifluorescence data suggested the increase in the mixing entropy and film relaxation rate. Finally, PM-IRRAS revealed the orientation of FB1 with the amine end (zwitterionic and negatively charged monolayers) or the tricarballylic acid end (positively charged monolayers) pointing to the air. The globular-extended conformational equilibrium of FB1 is dynamically defined by the membrane charge becoming a toxicity enhancing factor. The specificity of the toxin–protein interaction might also be perturbed by the FB1-induced remodeling of the membrane topography by affecting the raft-like platforms where membrane enzymes are considered to be located.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267970
Theumer, Martín Gustavo; Clop, Pedro Diego; Rubinstein, Héctor Ramón; Perillo, Maria Angelica; Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes; American Chemical Society; Journal of Physical Chemistry B; 116; 49; 12-2012; 14216-14227
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267970
identifier_str_mv Theumer, Martín Gustavo; Clop, Pedro Diego; Rubinstein, Héctor Ramón; Perillo, Maria Angelica; Effect of Surface Charge on the Interfacial Orientation and Conformation of FB1 in Model Membranes; American Chemical Society; Journal of Physical Chemistry B; 116; 49; 12-2012; 14216-14227
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jp306350k
info:eu-repo/semantics/altIdentifier/doi/10.1021/jp306350k
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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