Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea

Autores
Vassalli, Massimo; Penna, Antonella; Sbrana, Francesca; Casabianca, Silvia; Gjeci, Nikolla; Capellacci, Samuela; Asnaghi, Valentina; Ottaviani, Ennio; Giussani, Valentina; Pugliese, Laura; Jauzein, Cécile; Lemée, Rodolphe; Hachani, Mohamed Amine; Turki, Souad; Açaf, Laury; Saab, Marie Abboud-Abi; Fricke, Anna Lena; Mangialajo, Luisa; Bertolotto, Rosella; Totti, Cecilia; Accoroni, Stefano; Berdalet, Elisa; Vila, Magda; Chiantore, Mariachiara
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan-Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.
Fil: Vassalli, Massimo. Consiglio Nazionale delle Ricerche; Italia
Fil: Penna, Antonella. University of Urbino; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Sbrana, Francesca. Consiglio Nazionale delle Ricerche; Italia
Fil: Casabianca, Silvia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Gjeci, Nikolla. OnAir SRL; Italia
Fil: Capellacci, Samuela. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Asnaghi, Valentina. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Ottaviani, Ennio. OnAir SRL; Italia
Fil: Giussani, Valentina. Università degli Studi di Genova; Italia. Agenzia Regionale per la Protezione dell’Ambiente Ligure; Italia
Fil: Pugliese, Laura. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Jauzein, Cécile. Sorbonne Universités; Francia. Centre de Brest; Francia
Fil: Lemée, Rodolphe. Sorbonne Universités; Francia
Fil: Hachani, Mohamed Amine. Instm Centre la Goulette; Túnez
Fil: Turki, Souad. Instm Centre la Goulette; Túnez
Fil: Açaf, Laury. National Center for Marine Sciences; Líbano
Fil: Saab, Marie Abboud-Abi. National Center for Marine Sciences; Líbano
Fil: Fricke, Anna Lena. Université Côte d’Azur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Mangialajo, Luisa. Sorbonne Universités; Francia. Université Côte d’Azur; Francia
Fil: Bertolotto, Rosella. Agenzia Regionale per la Protezione dell’Ambiente Ligure; Italia
Fil: Totti, Cecilia. Università Politecnica delle Marche; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Accoroni, Stefano. Università Politecnica delle Marche; Italia
Fil: Berdalet, Elisa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Vila, Magda. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Chiantore, Mariachiara. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Materia
AUTOMATIC IDENTIFICATION
HARMFUL ALGAE
MEDITERRANEAN SEA
MOLECULAR RECOGNITION
MONITORING
OPTICAL RECOGNITION
OSTREOPSIS SPP.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93104
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93104
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean SeaVassalli, MassimoPenna, AntonellaSbrana, FrancescaCasabianca, SilviaGjeci, NikollaCapellacci, SamuelaAsnaghi, ValentinaOttaviani, EnnioGiussani, ValentinaPugliese, LauraJauzein, CécileLemée, RodolpheHachani, Mohamed AmineTurki, SouadAçaf, LaurySaab, Marie Abboud-AbiFricke, Anna LenaMangialajo, LuisaBertolotto, RosellaTotti, CeciliaAccoroni, StefanoBerdalet, ElisaVila, MagdaChiantore, MariachiaraAUTOMATIC IDENTIFICATIONHARMFUL ALGAEMEDITERRANEAN SEAMOLECULAR RECOGNITIONMONITORINGOPTICAL RECOGNITIONOSTREOPSIS SPP.https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan-Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.Fil: Vassalli, Massimo. Consiglio Nazionale delle Ricerche; ItaliaFil: Penna, Antonella. University of Urbino; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; ItaliaFil: Sbrana, Francesca. Consiglio Nazionale delle Ricerche; ItaliaFil: Casabianca, Silvia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; ItaliaFil: Gjeci, Nikolla. OnAir SRL; ItaliaFil: Capellacci, Samuela. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; ItaliaFil: Asnaghi, Valentina. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; ItaliaFil: Ottaviani, Ennio. OnAir SRL; ItaliaFil: Giussani, Valentina. Università degli Studi di Genova; Italia. Agenzia Regionale per la Protezione dell’Ambiente Ligure; ItaliaFil: Pugliese, Laura. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; ItaliaFil: Jauzein, Cécile. Sorbonne Universités; Francia. Centre de Brest; FranciaFil: Lemée, Rodolphe. Sorbonne Universités; FranciaFil: Hachani, Mohamed Amine. Instm Centre la Goulette; TúnezFil: Turki, Souad. Instm Centre la Goulette; TúnezFil: Açaf, Laury. National Center for Marine Sciences; LíbanoFil: Saab, Marie Abboud-Abi. National Center for Marine Sciences; LíbanoFil: Fricke, Anna Lena. Université Côte d’Azur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; ArgentinaFil: Mangialajo, Luisa. Sorbonne Universités; Francia. Université Côte d’Azur; FranciaFil: Bertolotto, Rosella. Agenzia Regionale per la Protezione dell’Ambiente Ligure; ItaliaFil: Totti, Cecilia. Università Politecnica delle Marche; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; ItaliaFil: Accoroni, Stefano. Università Politecnica delle Marche; ItaliaFil: Berdalet, Elisa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Vila, Magda. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; EspañaFil: Chiantore, Mariachiara. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; ItaliaElsevier Science2018-03-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93104Vassalli, Massimo; Penna, Antonella; Sbrana, Francesca; Casabianca, Silvia; Gjeci, Nikolla; et al.; Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea; Elsevier Science; Ecological Indicators; 85; 3-3-2018; 1092-11001470-160XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1470160X17304776info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2017.07.063info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:54:37Zoai:ri.conicet.gov.ar:11336/93104instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:54:38.015CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
title Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
spellingShingle Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
Vassalli, Massimo
AUTOMATIC IDENTIFICATION
HARMFUL ALGAE
MEDITERRANEAN SEA
MOLECULAR RECOGNITION
MONITORING
OPTICAL RECOGNITION
OSTREOPSIS SPP.
title_short Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
title_full Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
title_fullStr Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
title_full_unstemmed Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
title_sort Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
dc.creator.none.fl_str_mv Vassalli, Massimo
Penna, Antonella
Sbrana, Francesca
Casabianca, Silvia
Gjeci, Nikolla
Capellacci, Samuela
Asnaghi, Valentina
Ottaviani, Ennio
Giussani, Valentina
Pugliese, Laura
Jauzein, Cécile
Lemée, Rodolphe
Hachani, Mohamed Amine
Turki, Souad
Açaf, Laury
Saab, Marie Abboud-Abi
Fricke, Anna Lena
Mangialajo, Luisa
Bertolotto, Rosella
Totti, Cecilia
Accoroni, Stefano
Berdalet, Elisa
Vila, Magda
Chiantore, Mariachiara
author Vassalli, Massimo
author_facet Vassalli, Massimo
Penna, Antonella
Sbrana, Francesca
Casabianca, Silvia
Gjeci, Nikolla
Capellacci, Samuela
Asnaghi, Valentina
Ottaviani, Ennio
Giussani, Valentina
Pugliese, Laura
Jauzein, Cécile
Lemée, Rodolphe
Hachani, Mohamed Amine
Turki, Souad
Açaf, Laury
Saab, Marie Abboud-Abi
Fricke, Anna Lena
Mangialajo, Luisa
Bertolotto, Rosella
Totti, Cecilia
Accoroni, Stefano
Berdalet, Elisa
Vila, Magda
Chiantore, Mariachiara
author_role author
author2 Penna, Antonella
Sbrana, Francesca
Casabianca, Silvia
Gjeci, Nikolla
Capellacci, Samuela
Asnaghi, Valentina
Ottaviani, Ennio
Giussani, Valentina
Pugliese, Laura
Jauzein, Cécile
Lemée, Rodolphe
Hachani, Mohamed Amine
Turki, Souad
Açaf, Laury
Saab, Marie Abboud-Abi
Fricke, Anna Lena
Mangialajo, Luisa
Bertolotto, Rosella
Totti, Cecilia
Accoroni, Stefano
Berdalet, Elisa
Vila, Magda
Chiantore, Mariachiara
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUTOMATIC IDENTIFICATION
HARMFUL ALGAE
MEDITERRANEAN SEA
MOLECULAR RECOGNITION
MONITORING
OPTICAL RECOGNITION
OSTREOPSIS SPP.
topic AUTOMATIC IDENTIFICATION
HARMFUL ALGAE
MEDITERRANEAN SEA
MOLECULAR RECOGNITION
MONITORING
OPTICAL RECOGNITION
OSTREOPSIS SPP.
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan-Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.
Fil: Vassalli, Massimo. Consiglio Nazionale delle Ricerche; Italia
Fil: Penna, Antonella. University of Urbino; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Sbrana, Francesca. Consiglio Nazionale delle Ricerche; Italia
Fil: Casabianca, Silvia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Gjeci, Nikolla. OnAir SRL; Italia
Fil: Capellacci, Samuela. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Asnaghi, Valentina. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Ottaviani, Ennio. OnAir SRL; Italia
Fil: Giussani, Valentina. Università degli Studi di Genova; Italia. Agenzia Regionale per la Protezione dell’Ambiente Ligure; Italia
Fil: Pugliese, Laura. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia. University of Urbino; Italia
Fil: Jauzein, Cécile. Sorbonne Universités; Francia. Centre de Brest; Francia
Fil: Lemée, Rodolphe. Sorbonne Universités; Francia
Fil: Hachani, Mohamed Amine. Instm Centre la Goulette; Túnez
Fil: Turki, Souad. Instm Centre la Goulette; Túnez
Fil: Açaf, Laury. National Center for Marine Sciences; Líbano
Fil: Saab, Marie Abboud-Abi. National Center for Marine Sciences; Líbano
Fil: Fricke, Anna Lena. Université Côte d’Azur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina
Fil: Mangialajo, Luisa. Sorbonne Universités; Francia. Université Côte d’Azur; Francia
Fil: Bertolotto, Rosella. Agenzia Regionale per la Protezione dell’Ambiente Ligure; Italia
Fil: Totti, Cecilia. Università Politecnica delle Marche; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
Fil: Accoroni, Stefano. Università Politecnica delle Marche; Italia
Fil: Berdalet, Elisa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Vila, Magda. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Chiantore, Mariachiara. Università degli Studi di Genova; Italia. Consorzio Nazionale Interuniversitario per le Scienze del Mare; Italia
description This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan-Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93104
Vassalli, Massimo; Penna, Antonella; Sbrana, Francesca; Casabianca, Silvia; Gjeci, Nikolla; et al.; Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea; Elsevier Science; Ecological Indicators; 85; 3-3-2018; 1092-1100
1470-160X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93104
identifier_str_mv Vassalli, Massimo; Penna, Antonella; Sbrana, Francesca; Casabianca, Silvia; Gjeci, Nikolla; et al.; Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea; Elsevier Science; Ecological Indicators; 85; 3-3-2018; 1092-1100
1470-160X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1470160X17304776
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2017.07.063
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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