Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries

Autores
Molina, Gustavo Fabián; Cabalén, María Belén; Aranguren Abrate, Juan Pablo; Pino, Gustavo Ariel; Burrow, Michael Francis
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: To test the biological properties of a novel non-restorative treatmentmethod for arresting dentin caries based on silver nanoclusters (AgNCls)synthesized in polymethacrylic acid (PMAA).Methods: Synthesis of AgNCls was performed by photoreduction of AgNO3 inPMAA with 355 nm/wavelength light. AgNCls/PMAA was characterized byabsorption/fluorescence spectroscopy and optical and atomic force microscopy.The stability of the clusters in an aerated PMAA solution was evaluated by meansof fluorescence spectroscopy. Cytotoxicity was assessed using the MTT assayand antibacterial effect was determined for minimum inhibitory concentration(MIC), minimum bactericidal concentration (MBC) and colony forming (CFU) ofStreptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus).38% Silver Diammine Fluoride (SDF) was used for the control groups.Results: Chemical and structural identity of the clusters did not change within 9months; Cell viability of 92%–89% was found after 24–48 h respectively. MICand MBC were determined from 1:16 and 1:8 dilutions, respectively. Log CFUcounts of S. mutans, and L. acidophilus treated with AgNCls/PMAA (3.4 ppm ofsilver) were significantly lower than in the control groups and even lower thanwhen the same bacterial strains were treated with SDF (15,525 ppm of silver).Conclusions: AgNCls/PMAA presented chemical stability, acceptablecytotoxicity, and a potential antibacterial effect for strains associated withcaries lesions at very low concentrations of silver.
Fil: Molina, Gustavo Fabián. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Cabalén, María Belén. Universidad Católica de Córdoba. Facultad de Medicina; Argentina
Fil: Aranguren Abrate, Juan Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Pino, Gustavo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Universidad Nacional de Córdoba. Rectorado. Centro Laser de Ciencias Moleculares; Argentina
Fil: Burrow, Michael Francis. University Of Hong Kong; Hong Kong
Materia
odontologia
nanomateriales
caries
antibacterial
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/259160
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/259160
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological properties of a novel solution based on silver nanoclusters for arresting dentin cariesMolina, Gustavo FabiánCabalén, María BelénAranguren Abrate, Juan PabloPino, Gustavo ArielBurrow, Michael Francisodontologiananomaterialescariesantibacterialhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Objectives: To test the biological properties of a novel non-restorative treatmentmethod for arresting dentin caries based on silver nanoclusters (AgNCls)synthesized in polymethacrylic acid (PMAA).Methods: Synthesis of AgNCls was performed by photoreduction of AgNO3 inPMAA with 355 nm/wavelength light. AgNCls/PMAA was characterized byabsorption/fluorescence spectroscopy and optical and atomic force microscopy.The stability of the clusters in an aerated PMAA solution was evaluated by meansof fluorescence spectroscopy. Cytotoxicity was assessed using the MTT assayand antibacterial effect was determined for minimum inhibitory concentration(MIC), minimum bactericidal concentration (MBC) and colony forming (CFU) ofStreptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus).38% Silver Diammine Fluoride (SDF) was used for the control groups.Results: Chemical and structural identity of the clusters did not change within 9months; Cell viability of 92%–89% was found after 24–48 h respectively. MICand MBC were determined from 1:16 and 1:8 dilutions, respectively. Log CFUcounts of S. mutans, and L. acidophilus treated with AgNCls/PMAA (3.4 ppm ofsilver) were significantly lower than in the control groups and even lower thanwhen the same bacterial strains were treated with SDF (15,525 ppm of silver).Conclusions: AgNCls/PMAA presented chemical stability, acceptablecytotoxicity, and a potential antibacterial effect for strains associated withcaries lesions at very low concentrations of silver.Fil: Molina, Gustavo Fabián. Universidad Nacional de Córdoba. Facultad de Odontología; ArgentinaFil: Cabalén, María Belén. Universidad Católica de Córdoba. Facultad de Medicina; ArgentinaFil: Aranguren Abrate, Juan Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Pino, Gustavo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Universidad Nacional de Córdoba. Rectorado. Centro Laser de Ciencias Moleculares; ArgentinaFil: Burrow, Michael Francis. University Of Hong Kong; Hong KongFrontiers Media2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/259160Molina, Gustavo Fabián; Cabalén, María Belén; Aranguren Abrate, Juan Pablo; Pino, Gustavo Ariel; Burrow, Michael Francis; Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries; Frontiers Media; Frontiers in Oral Health; 5; 7-2024; 1-102673-4842CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2024.1408181/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/froh.2024.1408181info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:55:35Zoai:ri.conicet.gov.ar:11336/259160instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:55:35.611CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
title Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
spellingShingle Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
Molina, Gustavo Fabián
odontologia
nanomateriales
caries
antibacterial
title_short Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
title_full Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
title_fullStr Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
title_full_unstemmed Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
title_sort Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries
dc.creator.none.fl_str_mv Molina, Gustavo Fabián
Cabalén, María Belén
Aranguren Abrate, Juan Pablo
Pino, Gustavo Ariel
Burrow, Michael Francis
author Molina, Gustavo Fabián
author_facet Molina, Gustavo Fabián
Cabalén, María Belén
Aranguren Abrate, Juan Pablo
Pino, Gustavo Ariel
Burrow, Michael Francis
author_role author
author2 Cabalén, María Belén
Aranguren Abrate, Juan Pablo
Pino, Gustavo Ariel
Burrow, Michael Francis
author2_role author
author
author
author
dc.subject.none.fl_str_mv odontologia
nanomateriales
caries
antibacterial
topic odontologia
nanomateriales
caries
antibacterial
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: To test the biological properties of a novel non-restorative treatmentmethod for arresting dentin caries based on silver nanoclusters (AgNCls)synthesized in polymethacrylic acid (PMAA).Methods: Synthesis of AgNCls was performed by photoreduction of AgNO3 inPMAA with 355 nm/wavelength light. AgNCls/PMAA was characterized byabsorption/fluorescence spectroscopy and optical and atomic force microscopy.The stability of the clusters in an aerated PMAA solution was evaluated by meansof fluorescence spectroscopy. Cytotoxicity was assessed using the MTT assayand antibacterial effect was determined for minimum inhibitory concentration(MIC), minimum bactericidal concentration (MBC) and colony forming (CFU) ofStreptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus).38% Silver Diammine Fluoride (SDF) was used for the control groups.Results: Chemical and structural identity of the clusters did not change within 9months; Cell viability of 92%–89% was found after 24–48 h respectively. MICand MBC were determined from 1:16 and 1:8 dilutions, respectively. Log CFUcounts of S. mutans, and L. acidophilus treated with AgNCls/PMAA (3.4 ppm ofsilver) were significantly lower than in the control groups and even lower thanwhen the same bacterial strains were treated with SDF (15,525 ppm of silver).Conclusions: AgNCls/PMAA presented chemical stability, acceptablecytotoxicity, and a potential antibacterial effect for strains associated withcaries lesions at very low concentrations of silver.
Fil: Molina, Gustavo Fabián. Universidad Nacional de Córdoba. Facultad de Odontología; Argentina
Fil: Cabalén, María Belén. Universidad Católica de Córdoba. Facultad de Medicina; Argentina
Fil: Aranguren Abrate, Juan Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Pino, Gustavo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Fisicoquímica; Argentina. Universidad Nacional de Córdoba. Rectorado. Centro Laser de Ciencias Moleculares; Argentina
Fil: Burrow, Michael Francis. University Of Hong Kong; Hong Kong
description Objectives: To test the biological properties of a novel non-restorative treatmentmethod for arresting dentin caries based on silver nanoclusters (AgNCls)synthesized in polymethacrylic acid (PMAA).Methods: Synthesis of AgNCls was performed by photoreduction of AgNO3 inPMAA with 355 nm/wavelength light. AgNCls/PMAA was characterized byabsorption/fluorescence spectroscopy and optical and atomic force microscopy.The stability of the clusters in an aerated PMAA solution was evaluated by meansof fluorescence spectroscopy. Cytotoxicity was assessed using the MTT assayand antibacterial effect was determined for minimum inhibitory concentration(MIC), minimum bactericidal concentration (MBC) and colony forming (CFU) ofStreptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus).38% Silver Diammine Fluoride (SDF) was used for the control groups.Results: Chemical and structural identity of the clusters did not change within 9months; Cell viability of 92%–89% was found after 24–48 h respectively. MICand MBC were determined from 1:16 and 1:8 dilutions, respectively. Log CFUcounts of S. mutans, and L. acidophilus treated with AgNCls/PMAA (3.4 ppm ofsilver) were significantly lower than in the control groups and even lower thanwhen the same bacterial strains were treated with SDF (15,525 ppm of silver).Conclusions: AgNCls/PMAA presented chemical stability, acceptablecytotoxicity, and a potential antibacterial effect for strains associated withcaries lesions at very low concentrations of silver.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/259160
Molina, Gustavo Fabián; Cabalén, María Belén; Aranguren Abrate, Juan Pablo; Pino, Gustavo Ariel; Burrow, Michael Francis; Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries; Frontiers Media; Frontiers in Oral Health; 5; 7-2024; 1-10
2673-4842
CONICET Digital
CONICET
url http://hdl.handle.net/11336/259160
identifier_str_mv Molina, Gustavo Fabián; Cabalén, María Belén; Aranguren Abrate, Juan Pablo; Pino, Gustavo Ariel; Burrow, Michael Francis; Biological properties of a novel solution based on silver nanoclusters for arresting dentin caries; Frontiers Media; Frontiers in Oral Health; 5; 7-2024; 1-10
2673-4842
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2024.1408181/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/froh.2024.1408181
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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