Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
- Autores
- Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.
- Año de publicación
- 2000
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.
Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina
Fil: Lobo Castañón, M.J.. Universidad de Oviedo; España
Fil: Miranda Ordieres, A.J.. Universidad de Oviedo; España
Fil: Tuñón Blanco, P.. Universidad de Oviedo; España - Materia
-
AMPEROMETRY
NAD+ OXIDATION
SORBITOL BIOSENSOR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/97809
Ver los metadatos del registro completo
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Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrodeSaidman, Silvana BeatrizLobo Castañón, M.J.Miranda Ordieres, A.J.Tuñón Blanco, P.AMPEROMETRYNAD+ OXIDATIONSORBITOL BIOSENSORhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; ArgentinaFil: Lobo Castañón, M.J.. Universidad de Oviedo; EspañaFil: Miranda Ordieres, A.J.. Universidad de Oviedo; EspañaFil: Tuñón Blanco, P.. Universidad de Oviedo; EspañaElsevier Science2000-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97809Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-500003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-2670(00)01140-5info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267000011405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:25Zoai:ri.conicet.gov.ar:11336/97809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:25.535CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
title |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
spellingShingle |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode Saidman, Silvana Beatriz AMPEROMETRY NAD+ OXIDATION SORBITOL BIOSENSOR |
title_short |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
title_full |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
title_fullStr |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
title_full_unstemmed |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
title_sort |
Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode |
dc.creator.none.fl_str_mv |
Saidman, Silvana Beatriz Lobo Castañón, M.J. Miranda Ordieres, A.J. Tuñón Blanco, P. |
author |
Saidman, Silvana Beatriz |
author_facet |
Saidman, Silvana Beatriz Lobo Castañón, M.J. Miranda Ordieres, A.J. Tuñón Blanco, P. |
author_role |
author |
author2 |
Lobo Castañón, M.J. Miranda Ordieres, A.J. Tuñón Blanco, P. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
AMPEROMETRY NAD+ OXIDATION SORBITOL BIOSENSOR |
topic |
AMPEROMETRY NAD+ OXIDATION SORBITOL BIOSENSOR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M. Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina Fil: Lobo Castañón, M.J.. Universidad de Oviedo; España Fil: Miranda Ordieres, A.J.. Universidad de Oviedo; España Fil: Tuñón Blanco, P.. Universidad de Oviedo; España |
description |
A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/97809 Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-50 0003-2670 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/97809 |
identifier_str_mv |
Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-50 0003-2670 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-2670(00)01140-5 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267000011405 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614445008945152 |
score |
13.070432 |