Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode

Autores
Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.
Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina
Fil: Lobo Castañón, M.J.. Universidad de Oviedo; España
Fil: Miranda Ordieres, A.J.. Universidad de Oviedo; España
Fil: Tuñón Blanco, P.. Universidad de Oviedo; España
Materia
AMPEROMETRY
NAD+ OXIDATION
SORBITOL BIOSENSOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97809
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrodeSaidman, Silvana BeatrizLobo Castañón, M.J.Miranda Ordieres, A.J.Tuñón Blanco, P.AMPEROMETRYNAD+ OXIDATIONSORBITOL BIOSENSORhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; ArgentinaFil: Lobo Castañón, M.J.. Universidad de Oviedo; EspañaFil: Miranda Ordieres, A.J.. Universidad de Oviedo; EspañaFil: Tuñón Blanco, P.. Universidad de Oviedo; EspañaElsevier Science2000-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97809Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-500003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-2670(00)01140-5info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267000011405info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:13:59Zoai:ri.conicet.gov.ar:11336/97809instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:14:00.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
title Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
spellingShingle Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
Saidman, Silvana Beatriz
AMPEROMETRY
NAD+ OXIDATION
SORBITOL BIOSENSOR
title_short Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
title_full Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
title_fullStr Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
title_full_unstemmed Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
title_sort Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode
dc.creator.none.fl_str_mv Saidman, Silvana Beatriz
Lobo Castañón, M.J.
Miranda Ordieres, A.J.
Tuñón Blanco, P.
author Saidman, Silvana Beatriz
author_facet Saidman, Silvana Beatriz
Lobo Castañón, M.J.
Miranda Ordieres, A.J.
Tuñón Blanco, P.
author_role author
author2 Lobo Castañón, M.J.
Miranda Ordieres, A.J.
Tuñón Blanco, P.
author2_role author
author
author
dc.subject.none.fl_str_mv AMPEROMETRY
NAD+ OXIDATION
SORBITOL BIOSENSOR
topic AMPEROMETRY
NAD+ OXIDATION
SORBITOL BIOSENSOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.
Fil: Saidman, Silvana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química. Instituto de Ingeniería Electroquímica y Corrosión; Argentina
Fil: Lobo Castañón, M.J.. Universidad de Oviedo; España
Fil: Miranda Ordieres, A.J.. Universidad de Oviedo; España
Fil: Tuñón Blanco, P.. Universidad de Oviedo; España
description A biosensor based on modified carbon paste electrodes for the amperometric detection of D-sorbitol is described. Carbon paste electrodes were modified by D-sorbitol dehydrogenase (SDH) and nicotinamide adenine dinucleotide (NAD+) and coated with a non-conducting poly(o-phenylenediamine) (PPD) film. After partial oxidation of the immobilised NAD+ by applying a potential step f rom 0.0 to 1.2 V (SCE), the modified electrode allowed the amperometric detection of the NADH enzymatically obtained at an applied potential of 0.0 V. The resulting biosensor responded rapidly to sorbitol up to 8 x 10-4 M with a detection limit of 4 x 10-5 M.
publishDate 2000
dc.date.none.fl_str_mv 2000-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97809
Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-50
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97809
identifier_str_mv Saidman, Silvana Beatriz; Lobo Castañón, M.J.; Miranda Ordieres, A.J.; Tuñón Blanco, P.; Amperometric detection of D-sorbitol with NAD+-D-sorbitol dehydrogenase modified carbon paste electrode; Elsevier Science; Analytica Chimica Acta; 424; 1; 11-2000; 45-50
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0003-2670(00)01140-5
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267000011405
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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