Macrophage derived signalling regulates negatively the megakaryocyte compartment.

Autores
Landoni, Verónica Inés; Vermeulen, Elba Monica; Van Rooijen, N; Gómez, Sonia Alejandra; Palermo, Marina Sandra; Isturiz, Martín Amadeo; Alves Rosa, Maria Fernanda
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Megakaryocytopoiesis is the process by which stem cells go through a process of commitment, proliferation and differentiation leading to the production of platelets. In the mouse, this process is accomplished within the bone marrow (BM) and spleen microenvironment and is carried out by regulatory molecules and accessory cells including macrophages, fibroblasts and endothelial-like cells. Previously, we have reported that macrophage depletion following administration of liposomal clodronate (LIP-CLOD) provokes enhancement of both, megakaryocytopoiesis and thrombocytopoiesis. In this report, we investigated the changes in the compartment of megakaryocyte progenitor cells (MK-CFU), their correlation with plasmatic thrombopoietin (TPO) and TPO transcription levels after macrophage depletion. LIP-CLOD-treated mice showed an increase of the MK-CFU in BM and spleen. Concerning TPO plasma levels, kinetic studies revealed a 1.5- and 1.3-fold increase in the TPO concentration at 12 and 24 hr of treatment. We also show evidence of regulation of TPO transcription in the liver and spleen. Although empty liposomes also enhanced TPO gene regulation in these organs, transcriptional TPO up regulation correlated with an increase of protein synthesis only in those animals where macrophages were effectively removed. Taken together, these results suggest that BM and spleen macrophages derived signalling regulates negatively the megakaryocyte compartment
Fil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Van Rooijen, N. University of Amsterdam; Países Bajos
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alves Rosa, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Macrophage
Megakaryocyte
Inflammation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66745
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Macrophage derived signalling regulates negatively the megakaryocyte compartment.Landoni, Verónica InésVermeulen, Elba MonicaVan Rooijen, NGómez, Sonia AlejandraPalermo, Marina SandraIsturiz, Martín AmadeoAlves Rosa, Maria FernandaMacrophageMegakaryocyteInflammationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Megakaryocytopoiesis is the process by which stem cells go through a process of commitment, proliferation and differentiation leading to the production of platelets. In the mouse, this process is accomplished within the bone marrow (BM) and spleen microenvironment and is carried out by regulatory molecules and accessory cells including macrophages, fibroblasts and endothelial-like cells. Previously, we have reported that macrophage depletion following administration of liposomal clodronate (LIP-CLOD) provokes enhancement of both, megakaryocytopoiesis and thrombocytopoiesis. In this report, we investigated the changes in the compartment of megakaryocyte progenitor cells (MK-CFU), their correlation with plasmatic thrombopoietin (TPO) and TPO transcription levels after macrophage depletion. LIP-CLOD-treated mice showed an increase of the MK-CFU in BM and spleen. Concerning TPO plasma levels, kinetic studies revealed a 1.5- and 1.3-fold increase in the TPO concentration at 12 and 24 hr of treatment. We also show evidence of regulation of TPO transcription in the liver and spleen. Although empty liposomes also enhanced TPO gene regulation in these organs, transcriptional TPO up regulation correlated with an increase of protein synthesis only in those animals where macrophages were effectively removed. Taken together, these results suggest that BM and spleen macrophages derived signalling regulates negatively the megakaryocyte compartmentFil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Van Rooijen, N. University of Amsterdam; Países BajosFil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alves Rosa, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaC M B Association2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66745Landoni, Verónica Inés; Vermeulen, Elba Monica; Van Rooijen, N; Gómez, Sonia Alejandra; Palermo, Marina Sandra; et al.; Macrophage derived signalling regulates negatively the megakaryocyte compartment.; C M B Association; Cellular and Molecular Biology; 50; 12-2004; 667-6750145-5680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1170/80info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:33Zoai:ri.conicet.gov.ar:11336/66745instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:34.124CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Macrophage derived signalling regulates negatively the megakaryocyte compartment.
title Macrophage derived signalling regulates negatively the megakaryocyte compartment.
spellingShingle Macrophage derived signalling regulates negatively the megakaryocyte compartment.
Landoni, Verónica Inés
Macrophage
Megakaryocyte
Inflammation
title_short Macrophage derived signalling regulates negatively the megakaryocyte compartment.
title_full Macrophage derived signalling regulates negatively the megakaryocyte compartment.
title_fullStr Macrophage derived signalling regulates negatively the megakaryocyte compartment.
title_full_unstemmed Macrophage derived signalling regulates negatively the megakaryocyte compartment.
title_sort Macrophage derived signalling regulates negatively the megakaryocyte compartment.
dc.creator.none.fl_str_mv Landoni, Verónica Inés
Vermeulen, Elba Monica
Van Rooijen, N
Gómez, Sonia Alejandra
Palermo, Marina Sandra
Isturiz, Martín Amadeo
Alves Rosa, Maria Fernanda
author Landoni, Verónica Inés
author_facet Landoni, Verónica Inés
Vermeulen, Elba Monica
Van Rooijen, N
Gómez, Sonia Alejandra
Palermo, Marina Sandra
Isturiz, Martín Amadeo
Alves Rosa, Maria Fernanda
author_role author
author2 Vermeulen, Elba Monica
Van Rooijen, N
Gómez, Sonia Alejandra
Palermo, Marina Sandra
Isturiz, Martín Amadeo
Alves Rosa, Maria Fernanda
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Macrophage
Megakaryocyte
Inflammation
topic Macrophage
Megakaryocyte
Inflammation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Megakaryocytopoiesis is the process by which stem cells go through a process of commitment, proliferation and differentiation leading to the production of platelets. In the mouse, this process is accomplished within the bone marrow (BM) and spleen microenvironment and is carried out by regulatory molecules and accessory cells including macrophages, fibroblasts and endothelial-like cells. Previously, we have reported that macrophage depletion following administration of liposomal clodronate (LIP-CLOD) provokes enhancement of both, megakaryocytopoiesis and thrombocytopoiesis. In this report, we investigated the changes in the compartment of megakaryocyte progenitor cells (MK-CFU), their correlation with plasmatic thrombopoietin (TPO) and TPO transcription levels after macrophage depletion. LIP-CLOD-treated mice showed an increase of the MK-CFU in BM and spleen. Concerning TPO plasma levels, kinetic studies revealed a 1.5- and 1.3-fold increase in the TPO concentration at 12 and 24 hr of treatment. We also show evidence of regulation of TPO transcription in the liver and spleen. Although empty liposomes also enhanced TPO gene regulation in these organs, transcriptional TPO up regulation correlated with an increase of protein synthesis only in those animals where macrophages were effectively removed. Taken together, these results suggest that BM and spleen macrophages derived signalling regulates negatively the megakaryocyte compartment
Fil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Van Rooijen, N. University of Amsterdam; Países Bajos
Fil: Gómez, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alves Rosa, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Megakaryocytopoiesis is the process by which stem cells go through a process of commitment, proliferation and differentiation leading to the production of platelets. In the mouse, this process is accomplished within the bone marrow (BM) and spleen microenvironment and is carried out by regulatory molecules and accessory cells including macrophages, fibroblasts and endothelial-like cells. Previously, we have reported that macrophage depletion following administration of liposomal clodronate (LIP-CLOD) provokes enhancement of both, megakaryocytopoiesis and thrombocytopoiesis. In this report, we investigated the changes in the compartment of megakaryocyte progenitor cells (MK-CFU), their correlation with plasmatic thrombopoietin (TPO) and TPO transcription levels after macrophage depletion. LIP-CLOD-treated mice showed an increase of the MK-CFU in BM and spleen. Concerning TPO plasma levels, kinetic studies revealed a 1.5- and 1.3-fold increase in the TPO concentration at 12 and 24 hr of treatment. We also show evidence of regulation of TPO transcription in the liver and spleen. Although empty liposomes also enhanced TPO gene regulation in these organs, transcriptional TPO up regulation correlated with an increase of protein synthesis only in those animals where macrophages were effectively removed. Taken together, these results suggest that BM and spleen macrophages derived signalling regulates negatively the megakaryocyte compartment
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66745
Landoni, Verónica Inés; Vermeulen, Elba Monica; Van Rooijen, N; Gómez, Sonia Alejandra; Palermo, Marina Sandra; et al.; Macrophage derived signalling regulates negatively the megakaryocyte compartment.; C M B Association; Cellular and Molecular Biology; 50; 12-2004; 667-675
0145-5680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66745
identifier_str_mv Landoni, Verónica Inés; Vermeulen, Elba Monica; Van Rooijen, N; Gómez, Sonia Alejandra; Palermo, Marina Sandra; et al.; Macrophage derived signalling regulates negatively the megakaryocyte compartment.; C M B Association; Cellular and Molecular Biology; 50; 12-2004; 667-675
0145-5680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1170/80
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv C M B Association
publisher.none.fl_str_mv C M B Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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