Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture
- Autores
- Zuqueli, Romina; Prieto, Claudio; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater.
Fil: Zuqueli, Romina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina
Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina - Materia
-
RH-IFN-BETA
CHO
ZINC - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/107380
Ver los metadatos del registro completo
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Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell cultureZuqueli, RominaPrieto, ClaudioEtcheverrigaray, MarinaKratje, Ricardo BertoldoRH-IFN-BETACHOZINChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater.Fil: Zuqueli, Romina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaFil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; ArgentinaPlanta Piloto de Ingeniería Química2006-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107380Zuqueli, Romina; Prieto, Claudio; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo; Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture; Planta Piloto de Ingeniería Química; Latin American Applied Research; 36; 6-2006; 321-3270327-07931851-8796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-07932006000400018&lng=es&nrm=isoinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:07:37Zoai:ri.conicet.gov.ar:11336/107380instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:07:38.029CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| title |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| spellingShingle |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture Zuqueli, Romina RH-IFN-BETA CHO ZINC |
| title_short |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| title_full |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| title_fullStr |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| title_full_unstemmed |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| title_sort |
Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture |
| dc.creator.none.fl_str_mv |
Zuqueli, Romina Prieto, Claudio Etcheverrigaray, Marina Kratje, Ricardo Bertoldo |
| author |
Zuqueli, Romina |
| author_facet |
Zuqueli, Romina Prieto, Claudio Etcheverrigaray, Marina Kratje, Ricardo Bertoldo |
| author_role |
author |
| author2 |
Prieto, Claudio Etcheverrigaray, Marina Kratje, Ricardo Bertoldo |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
RH-IFN-BETA CHO ZINC |
| topic |
RH-IFN-BETA CHO ZINC |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater. Fil: Zuqueli, Romina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Cultivos Celulares; Argentina |
| description |
Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/107380 Zuqueli, Romina; Prieto, Claudio; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo; Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture; Planta Piloto de Ingeniería Química; Latin American Applied Research; 36; 6-2006; 321-327 0327-0793 1851-8796 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/107380 |
| identifier_str_mv |
Zuqueli, Romina; Prieto, Claudio; Etcheverrigaray, Marina; Kratje, Ricardo Bertoldo; Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN production by mammalian cell culture; Planta Piloto de Ingeniería Química; Latin American Applied Research; 36; 6-2006; 321-327 0327-0793 1851-8796 CONICET Digital CONICET |
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eng |
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eng |
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Planta Piloto de Ingeniería Química |
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Planta Piloto de Ingeniería Química |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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