Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)

Autores
Belaich, Mariano Nicolas; Buldain, Daniel Cornelio; Ghiringhelli, Pablo Daniel; Hyman, Bradley; Micieli, Maria Victoria; Achinelly, Maria Fernanda
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strelkovimermis spiculatus is an obligate parasite of mosquito larvae that breeds in environments of permanent flooding or in habitats subjected to periods of desiccation. Differences in epizootic levels within these habitats likely are due to variation at the genetic level. We evaluated whether nucleotide sequence variation levels might provide a basis for the development of molecular markers that correlate with infectivity phenotypes characteristic of different populations of S. spiculatus from La Plata, Buenos Aires province, Argentina. Partial DNA sequences from the nuclear 18S gene and the mitochondrial nd4 gene were analyzed. Nucleotide sequences from nematodes collected from temporary ponds (site 4), when aligned with sequences obtained from isolates found in permanent (site 1 and site 2) and temporary environments (site 3), revealed a modest number of substitutions the 18S and nd4 genes over short sequence expanses. Our results indicate that an expanded sampling of both nuclear and mitochondrial genes, with a special reference to nuclear genes encoding host range infectivity phenotypes, may be useful in the development of biomarkers for studying the population dynamics of S. spiculatus, and for correlating isolates with phenotypic traits useful for durable biological control practices.
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Buldain, Daniel Cornelio. Universidad Nacional de La Plata; Argentina
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Hyman, Bradley. University of California; Estados Unidos
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Materia
Nucleotide Differentiation
Mosquito
Strelkovimermis Spiculatus
Nematode
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10857
Acceder
id CONICETDig_13ee8c08385efe03394901137d0f34b5
oai_identifier_str oai:ri.conicet.gov.ar:11336/10857
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)Belaich, Mariano NicolasBuldain, Daniel CornelioGhiringhelli, Pablo DanielHyman, BradleyMicieli, Maria VictoriaAchinelly, Maria FernandaNucleotide DifferentiationMosquitoStrelkovimermis SpiculatusNematodehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Strelkovimermis spiculatus is an obligate parasite of mosquito larvae that breeds in environments of permanent flooding or in habitats subjected to periods of desiccation. Differences in epizootic levels within these habitats likely are due to variation at the genetic level. We evaluated whether nucleotide sequence variation levels might provide a basis for the development of molecular markers that correlate with infectivity phenotypes characteristic of different populations of S. spiculatus from La Plata, Buenos Aires province, Argentina. Partial DNA sequences from the nuclear 18S gene and the mitochondrial nd4 gene were analyzed. Nucleotide sequences from nematodes collected from temporary ponds (site 4), when aligned with sequences obtained from isolates found in permanent (site 1 and site 2) and temporary environments (site 3), revealed a modest number of substitutions the 18S and nd4 genes over short sequence expanses. Our results indicate that an expanded sampling of both nuclear and mitochondrial genes, with a special reference to nuclear genes encoding host range infectivity phenotypes, may be useful in the development of biomarkers for studying the population dynamics of S. spiculatus, and for correlating isolates with phenotypic traits useful for durable biological control practices.Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; ArgentinaFil: Buldain, Daniel Cornelio. Universidad Nacional de La Plata; ArgentinaFil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; ArgentinaFil: Hyman, Bradley. University of California; Estados UnidosFil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaFil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); ArgentinaSoc Vector Ecology2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10857Belaich, Mariano Nicolas; Buldain, Daniel Cornelio; Ghiringhelli, Pablo Daniel; Hyman, Bradley; Micieli, Maria Victoria; et al.; Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae); Soc Vector Ecology; Journal Of Vector Ecology; 40; 2; 12-2015; 415-4181081-1710enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jvec.12183/fullinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jvec.12183info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:50Zoai:ri.conicet.gov.ar:11336/10857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:51.17CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
title Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
spellingShingle Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
Belaich, Mariano Nicolas
Nucleotide Differentiation
Mosquito
Strelkovimermis Spiculatus
Nematode
title_short Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
title_full Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
title_fullStr Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
title_full_unstemmed Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
title_sort Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae)
dc.creator.none.fl_str_mv Belaich, Mariano Nicolas
Buldain, Daniel Cornelio
Ghiringhelli, Pablo Daniel
Hyman, Bradley
Micieli, Maria Victoria
Achinelly, Maria Fernanda
author Belaich, Mariano Nicolas
author_facet Belaich, Mariano Nicolas
Buldain, Daniel Cornelio
Ghiringhelli, Pablo Daniel
Hyman, Bradley
Micieli, Maria Victoria
Achinelly, Maria Fernanda
author_role author
author2 Buldain, Daniel Cornelio
Ghiringhelli, Pablo Daniel
Hyman, Bradley
Micieli, Maria Victoria
Achinelly, Maria Fernanda
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nucleotide Differentiation
Mosquito
Strelkovimermis Spiculatus
Nematode
topic Nucleotide Differentiation
Mosquito
Strelkovimermis Spiculatus
Nematode
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Strelkovimermis spiculatus is an obligate parasite of mosquito larvae that breeds in environments of permanent flooding or in habitats subjected to periods of desiccation. Differences in epizootic levels within these habitats likely are due to variation at the genetic level. We evaluated whether nucleotide sequence variation levels might provide a basis for the development of molecular markers that correlate with infectivity phenotypes characteristic of different populations of S. spiculatus from La Plata, Buenos Aires province, Argentina. Partial DNA sequences from the nuclear 18S gene and the mitochondrial nd4 gene were analyzed. Nucleotide sequences from nematodes collected from temporary ponds (site 4), when aligned with sequences obtained from isolates found in permanent (site 1 and site 2) and temporary environments (site 3), revealed a modest number of substitutions the 18S and nd4 genes over short sequence expanses. Our results indicate that an expanded sampling of both nuclear and mitochondrial genes, with a special reference to nuclear genes encoding host range infectivity phenotypes, may be useful in the development of biomarkers for studying the population dynamics of S. spiculatus, and for correlating isolates with phenotypic traits useful for durable biological control practices.
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Buldain, Daniel Cornelio. Universidad Nacional de La Plata; Argentina
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Hyman, Bradley. University of California; Estados Unidos
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina
description Strelkovimermis spiculatus is an obligate parasite of mosquito larvae that breeds in environments of permanent flooding or in habitats subjected to periods of desiccation. Differences in epizootic levels within these habitats likely are due to variation at the genetic level. We evaluated whether nucleotide sequence variation levels might provide a basis for the development of molecular markers that correlate with infectivity phenotypes characteristic of different populations of S. spiculatus from La Plata, Buenos Aires province, Argentina. Partial DNA sequences from the nuclear 18S gene and the mitochondrial nd4 gene were analyzed. Nucleotide sequences from nematodes collected from temporary ponds (site 4), when aligned with sequences obtained from isolates found in permanent (site 1 and site 2) and temporary environments (site 3), revealed a modest number of substitutions the 18S and nd4 genes over short sequence expanses. Our results indicate that an expanded sampling of both nuclear and mitochondrial genes, with a special reference to nuclear genes encoding host range infectivity phenotypes, may be useful in the development of biomarkers for studying the population dynamics of S. spiculatus, and for correlating isolates with phenotypic traits useful for durable biological control practices.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10857
Belaich, Mariano Nicolas; Buldain, Daniel Cornelio; Ghiringhelli, Pablo Daniel; Hyman, Bradley; Micieli, Maria Victoria; et al.; Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae); Soc Vector Ecology; Journal Of Vector Ecology; 40; 2; 12-2015; 415-418
1081-1710
url http://hdl.handle.net/11336/10857
identifier_str_mv Belaich, Mariano Nicolas; Buldain, Daniel Cornelio; Ghiringhelli, Pablo Daniel; Hyman, Bradley; Micieli, Maria Victoria; et al.; Nucleotide sequence differentiation of argentine isolates of the mosquito parasitic nematode Strelkovimermis spiculatus (Nematode: Mermithidae); Soc Vector Ecology; Journal Of Vector Ecology; 40; 2; 12-2015; 415-418
1081-1710
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jvec.12183/full
info:eu-repo/semantics/altIdentifier/doi/10.1111/jvec.12183
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Soc Vector Ecology
publisher.none.fl_str_mv Soc Vector Ecology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269663267913728
score 13.13397

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