Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control

Autores
Achinelly, Maria Fernanda; Micieli, Maria Victoria
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strelkovimermis spiculatus preparasite (J2) infectivity and postparasite (J4) production in rearing pans using a laboratory host, second instar Aedes aegypti mosquitoes. First, we evaluated efficiency of postparasite production by testing increasing J2 concentrations (preparasite:host ratios). Using 10 000 host larvae per rearing pan, we obtained the maximum yield of J4s (3.50 g), number of J4s/larva (3.1), and parasitism frequency (94.9 ± 9.5%) at a 10:1 ratio of J2/larva, although the 10:1 ratio resulted in significantly a male-biased sex ratio. The highest percentage of J2s emerging as J4s was 32% at 1:1 J2s/larva. Second, postparasites were evaluated as an inoculation method using two culture conditions (cultures dessicated or permanently flooded) reflecting two categories of mosquito habitats. The J4´s were allowed to develop to adults, mate, and oviposit in the substrate. The J2s from flooded cultures hatched from eggs at the third week and continued in continuously flooded habitat for up to 30 weeks, with a total J2 production of 6.2 x 10 per rearing pan. Maximum yield of J2s in dessicated cultures occurred on the first flooding (5 weeks), but preparasite emergence continued in subsequent drying-flooding cycles for ten weeks. Total J2 production from intermittently-flooded habitat was 4.5 x 10 per rearing pan. The J2s were always infective for mosquito larvae under both conditions. Either S. spiculatus preparasites or postparasites could be utilized for mosquito biological control in temporary or permanent mosquito habitats, and strategies for this are discussed.
Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Materia
CULICIDAE
MERMITHIDAE
WILD MOSQUITOES
OCHLEROTATUS ALBIFASCIATUS
STRELKOVIMERMIS SPICULATUS
BIOREGULATOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101921
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/101921
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological controlAchinelly, Maria FernandaMicieli, Maria VictoriaCULICIDAEMERMITHIDAEWILD MOSQUITOESOCHLEROTATUS ALBIFASCIATUSSTRELKOVIMERMIS SPICULATUSBIOREGULATORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Strelkovimermis spiculatus preparasite (J2) infectivity and postparasite (J4) production in rearing pans using a laboratory host, second instar Aedes aegypti mosquitoes. First, we evaluated efficiency of postparasite production by testing increasing J2 concentrations (preparasite:host ratios). Using 10 000 host larvae per rearing pan, we obtained the maximum yield of J4s (3.50 g), number of J4s/larva (3.1), and parasitism frequency (94.9 ± 9.5%) at a 10:1 ratio of J2/larva, although the 10:1 ratio resulted in significantly a male-biased sex ratio. The highest percentage of J2s emerging as J4s was 32% at 1:1 J2s/larva. Second, postparasites were evaluated as an inoculation method using two culture conditions (cultures dessicated or permanently flooded) reflecting two categories of mosquito habitats. The J4´s were allowed to develop to adults, mate, and oviposit in the substrate. The J2s from flooded cultures hatched from eggs at the third week and continued in continuously flooded habitat for up to 30 weeks, with a total J2 production of 6.2 x 10 per rearing pan. Maximum yield of J2s in dessicated cultures occurred on the first flooding (5 weeks), but preparasite emergence continued in subsequent drying-flooding cycles for ten weeks. Total J2 production from intermittently-flooded habitat was 4.5 x 10 per rearing pan. The J2s were always infective for mosquito larvae under both conditions. Either S. spiculatus preparasites or postparasites could be utilized for mosquito biological control in temporary or permanent mosquito habitats, and strategies for this are discussed.Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaAcademic Press Inc Elsevier Science2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101921Achinelly, Maria Fernanda; Micieli, Maria Victoria; Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control; Academic Press Inc Elsevier Science; Biological Control; 57; 1-2011; 31-361049-9644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2010.12.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S104996441000263Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:54Zoai:ri.conicet.gov.ar:11336/101921instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:54.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
title Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
spellingShingle Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
Achinelly, Maria Fernanda
CULICIDAE
MERMITHIDAE
WILD MOSQUITOES
OCHLEROTATUS ALBIFASCIATUS
STRELKOVIMERMIS SPICULATUS
BIOREGULATOR
title_short Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
title_full Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
title_fullStr Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
title_full_unstemmed Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
title_sort Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control
dc.creator.none.fl_str_mv Achinelly, Maria Fernanda
Micieli, Maria Victoria
author Achinelly, Maria Fernanda
author_facet Achinelly, Maria Fernanda
Micieli, Maria Victoria
author_role author
author2 Micieli, Maria Victoria
author2_role author
dc.subject.none.fl_str_mv CULICIDAE
MERMITHIDAE
WILD MOSQUITOES
OCHLEROTATUS ALBIFASCIATUS
STRELKOVIMERMIS SPICULATUS
BIOREGULATOR
topic CULICIDAE
MERMITHIDAE
WILD MOSQUITOES
OCHLEROTATUS ALBIFASCIATUS
STRELKOVIMERMIS SPICULATUS
BIOREGULATOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Strelkovimermis spiculatus preparasite (J2) infectivity and postparasite (J4) production in rearing pans using a laboratory host, second instar Aedes aegypti mosquitoes. First, we evaluated efficiency of postparasite production by testing increasing J2 concentrations (preparasite:host ratios). Using 10 000 host larvae per rearing pan, we obtained the maximum yield of J4s (3.50 g), number of J4s/larva (3.1), and parasitism frequency (94.9 ± 9.5%) at a 10:1 ratio of J2/larva, although the 10:1 ratio resulted in significantly a male-biased sex ratio. The highest percentage of J2s emerging as J4s was 32% at 1:1 J2s/larva. Second, postparasites were evaluated as an inoculation method using two culture conditions (cultures dessicated or permanently flooded) reflecting two categories of mosquito habitats. The J4´s were allowed to develop to adults, mate, and oviposit in the substrate. The J2s from flooded cultures hatched from eggs at the third week and continued in continuously flooded habitat for up to 30 weeks, with a total J2 production of 6.2 x 10 per rearing pan. Maximum yield of J2s in dessicated cultures occurred on the first flooding (5 weeks), but preparasite emergence continued in subsequent drying-flooding cycles for ten weeks. Total J2 production from intermittently-flooded habitat was 4.5 x 10 per rearing pan. The J2s were always infective for mosquito larvae under both conditions. Either S. spiculatus preparasites or postparasites could be utilized for mosquito biological control in temporary or permanent mosquito habitats, and strategies for this are discussed.
Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Micieli, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
description Strelkovimermis spiculatus preparasite (J2) infectivity and postparasite (J4) production in rearing pans using a laboratory host, second instar Aedes aegypti mosquitoes. First, we evaluated efficiency of postparasite production by testing increasing J2 concentrations (preparasite:host ratios). Using 10 000 host larvae per rearing pan, we obtained the maximum yield of J4s (3.50 g), number of J4s/larva (3.1), and parasitism frequency (94.9 ± 9.5%) at a 10:1 ratio of J2/larva, although the 10:1 ratio resulted in significantly a male-biased sex ratio. The highest percentage of J2s emerging as J4s was 32% at 1:1 J2s/larva. Second, postparasites were evaluated as an inoculation method using two culture conditions (cultures dessicated or permanently flooded) reflecting two categories of mosquito habitats. The J4´s were allowed to develop to adults, mate, and oviposit in the substrate. The J2s from flooded cultures hatched from eggs at the third week and continued in continuously flooded habitat for up to 30 weeks, with a total J2 production of 6.2 x 10 per rearing pan. Maximum yield of J2s in dessicated cultures occurred on the first flooding (5 weeks), but preparasite emergence continued in subsequent drying-flooding cycles for ten weeks. Total J2 production from intermittently-flooded habitat was 4.5 x 10 per rearing pan. The J2s were always infective for mosquito larvae under both conditions. Either S. spiculatus preparasites or postparasites could be utilized for mosquito biological control in temporary or permanent mosquito habitats, and strategies for this are discussed.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101921
Achinelly, Maria Fernanda; Micieli, Maria Victoria; Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control; Academic Press Inc Elsevier Science; Biological Control; 57; 1-2011; 31-36
1049-9644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101921
identifier_str_mv Achinelly, Maria Fernanda; Micieli, Maria Victoria; Optimizing laboratory production of Strelkovimermis spiculatus (Nematoda: Mermithidae) with a discussion of potential release strategies for mosquito biological control; Academic Press Inc Elsevier Science; Biological Control; 57; 1-2011; 31-36
1049-9644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biocontrol.2010.12.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S104996441000263X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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