Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells

Autores
Cormick, Maria Paula; Quiroga, Ezequiel Dario; Bertolotti, Sonia Graciela; Alvarez, María Gabriela; Durantini, Edgardo Néstor
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The photodynamic mechanism of action induced by 5-(4-trifluorophenyl)-10, 15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP 4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP 4+) was investigated on Candida albicans cells. These cationic porphyrins are effective photosensitizers, producing a ∼5 log decrease of cell survival when the cultures are incubated with 5 μM photosensitizer and irradiated for 30 min with visible light. Studies under anoxic conditions indicated that oxygen is necessary for the mechanism of action of photodynamic inactivation of this yeast. Furthermore, photoinactivation of C. albicans cells was negligible in the presence of 100 mM azide ion, whereas the photocytotoxicity induced by these porphyrins increased in D2O. In contrast, the addition of 100 mM mannitol produced a negligible effect on the cellular phototoxicity. On the other hand, in vitro direct observation of singlet molecular oxygen, O2(1Δg) phosphorescence at 1270 nm was analyzed using C. albicans in D2O. A shorter lifetime of O2(1Δg) was found in yeast cellular suspensions. These cationic porphyrins bind strongly to C. albicans cells and the O2(1Δg) generated inside the cells is rapidly quenched by the biomolecules of the cellular microenvironment. Therefore, the results indicate that these cationic porphyrins appear to act as photosensitizers mainly via the intermediacy of O 2(1Δg).
Fil: Cormick, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bertolotti, Sonia Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
PHOTODYNAMIC INACTIVATION
PHOTOSENSITIZERS
SINGLET OXYGEN
CANDIDA ALBICANS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192899
Acceder
id CONICETDig_13e156be88c93da028c396bef1b639a0
oai_identifier_str oai:ri.conicet.gov.ar:11336/192899
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cellsCormick, Maria PaulaQuiroga, Ezequiel DarioBertolotti, Sonia GracielaAlvarez, María GabrielaDurantini, Edgardo NéstorPHOTODYNAMIC INACTIVATIONPHOTOSENSITIZERSSINGLET OXYGENCANDIDA ALBICANShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The photodynamic mechanism of action induced by 5-(4-trifluorophenyl)-10, 15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP 4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP 4+) was investigated on Candida albicans cells. These cationic porphyrins are effective photosensitizers, producing a ∼5 log decrease of cell survival when the cultures are incubated with 5 μM photosensitizer and irradiated for 30 min with visible light. Studies under anoxic conditions indicated that oxygen is necessary for the mechanism of action of photodynamic inactivation of this yeast. Furthermore, photoinactivation of C. albicans cells was negligible in the presence of 100 mM azide ion, whereas the photocytotoxicity induced by these porphyrins increased in D2O. In contrast, the addition of 100 mM mannitol produced a negligible effect on the cellular phototoxicity. On the other hand, in vitro direct observation of singlet molecular oxygen, O2(1Δg) phosphorescence at 1270 nm was analyzed using C. albicans in D2O. A shorter lifetime of O2(1Δg) was found in yeast cellular suspensions. These cationic porphyrins bind strongly to C. albicans cells and the O2(1Δg) generated inside the cells is rapidly quenched by the biomolecules of the cellular microenvironment. Therefore, the results indicate that these cationic porphyrins appear to act as photosensitizers mainly via the intermediacy of O 2(1Δg).Fil: Cormick, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Bertolotti, Sonia Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaRoyal Society of Chemistry2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192899Cormick, Maria Paula; Quiroga, Ezequiel Dario; Bertolotti, Sonia Graciela; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 10; 10; 6-2011; 1556-15611474-905X1474-9092CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/pp/c1pp05074einfo:eu-repo/semantics/altIdentifier/doi/10.1039/C1PP05074Einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:56Zoai:ri.conicet.gov.ar:11336/192899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:56.988CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
title Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
spellingShingle Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
Cormick, Maria Paula
PHOTODYNAMIC INACTIVATION
PHOTOSENSITIZERS
SINGLET OXYGEN
CANDIDA ALBICANS
title_short Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
title_full Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
title_fullStr Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
title_full_unstemmed Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
title_sort Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells
dc.creator.none.fl_str_mv Cormick, Maria Paula
Quiroga, Ezequiel Dario
Bertolotti, Sonia Graciela
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author Cormick, Maria Paula
author_facet Cormick, Maria Paula
Quiroga, Ezequiel Dario
Bertolotti, Sonia Graciela
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author_role author
author2 Quiroga, Ezequiel Dario
Bertolotti, Sonia Graciela
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHOTODYNAMIC INACTIVATION
PHOTOSENSITIZERS
SINGLET OXYGEN
CANDIDA ALBICANS
topic PHOTODYNAMIC INACTIVATION
PHOTOSENSITIZERS
SINGLET OXYGEN
CANDIDA ALBICANS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The photodynamic mechanism of action induced by 5-(4-trifluorophenyl)-10, 15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP 4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP 4+) was investigated on Candida albicans cells. These cationic porphyrins are effective photosensitizers, producing a ∼5 log decrease of cell survival when the cultures are incubated with 5 μM photosensitizer and irradiated for 30 min with visible light. Studies under anoxic conditions indicated that oxygen is necessary for the mechanism of action of photodynamic inactivation of this yeast. Furthermore, photoinactivation of C. albicans cells was negligible in the presence of 100 mM azide ion, whereas the photocytotoxicity induced by these porphyrins increased in D2O. In contrast, the addition of 100 mM mannitol produced a negligible effect on the cellular phototoxicity. On the other hand, in vitro direct observation of singlet molecular oxygen, O2(1Δg) phosphorescence at 1270 nm was analyzed using C. albicans in D2O. A shorter lifetime of O2(1Δg) was found in yeast cellular suspensions. These cationic porphyrins bind strongly to C. albicans cells and the O2(1Δg) generated inside the cells is rapidly quenched by the biomolecules of the cellular microenvironment. Therefore, the results indicate that these cationic porphyrins appear to act as photosensitizers mainly via the intermediacy of O 2(1Δg).
Fil: Cormick, Maria Paula. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Bertolotti, Sonia Graciela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Alvarez, María Gabriela. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description The photodynamic mechanism of action induced by 5-(4-trifluorophenyl)-10, 15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl)porphyrin (TMAP 4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl)porphyrin (TMPyP 4+) was investigated on Candida albicans cells. These cationic porphyrins are effective photosensitizers, producing a ∼5 log decrease of cell survival when the cultures are incubated with 5 μM photosensitizer and irradiated for 30 min with visible light. Studies under anoxic conditions indicated that oxygen is necessary for the mechanism of action of photodynamic inactivation of this yeast. Furthermore, photoinactivation of C. albicans cells was negligible in the presence of 100 mM azide ion, whereas the photocytotoxicity induced by these porphyrins increased in D2O. In contrast, the addition of 100 mM mannitol produced a negligible effect on the cellular phototoxicity. On the other hand, in vitro direct observation of singlet molecular oxygen, O2(1Δg) phosphorescence at 1270 nm was analyzed using C. albicans in D2O. A shorter lifetime of O2(1Δg) was found in yeast cellular suspensions. These cationic porphyrins bind strongly to C. albicans cells and the O2(1Δg) generated inside the cells is rapidly quenched by the biomolecules of the cellular microenvironment. Therefore, the results indicate that these cationic porphyrins appear to act as photosensitizers mainly via the intermediacy of O 2(1Δg).
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192899
Cormick, Maria Paula; Quiroga, Ezequiel Dario; Bertolotti, Sonia Graciela; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 10; 10; 6-2011; 1556-1561
1474-905X
1474-9092
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192899
identifier_str_mv Cormick, Maria Paula; Quiroga, Ezequiel Dario; Bertolotti, Sonia Graciela; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic insight of the photodynamic effect induced by tri- and tetra-cationic porphyrins on Candida albicans cells; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 10; 10; 6-2011; 1556-1561
1474-905X
1474-9092
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2011/pp/c1pp05074e
info:eu-repo/semantics/altIdentifier/doi/10.1039/C1PP05074E
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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