First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients

Autores
Vera, Marina Cecilia; Biancardi, Maria Eugenia; Bottai, Hebe María; Lugano, Maria Cristina; Quiroga, Ariel Dario; Pisani, Gerardo Bruno; Quintana, Alejandra Beatriz
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: the shortage of donors of hepatocyte transplantation therapy led to the use of so-called marginal donors. Some donors may have a hepatic illnesses that is associated with hepatic preneoplasia with foci of altered hepatocytes (FAH). Aims: to determine whether recipients developed FAH upon transplantation with hepatocytes from a preneoplastic liver and whether FAH progresses to a preneoplastic hepatocyte- derived tumor (PHDT), up to 60 days after transplantation. Material and methods: male Wistar adult rats were used as donors and recipients. Donors underwent a 2-phase model of liver preneoplasia for hepatocyte isolation. Recipients underwent a partial two thirds hepatectomy and received 150,000 hepatocytes. Recipients were euthanized seven and 60 days after transplantation. The number of FAH per liver area, percentage of liver occupied by FAH, the hepatic enzymatic profile, the percentage of prothrombin time (PT), the proliferative index (PI) and liver morphology were analyzed. Results: recipients developed few and very isolated FAH. No statistical differences were found between hepatic enzyme activities and PT. There were no differences between the groups with regard to the number of FAH per liver area and percentage of liver occupied by FAH after 60 days. The PI decreased on day 60 compared to day seven. No morphological alterations were found. Conclusions: recipients developed few FAH that did not increase in number or size, nor did they progress to PHDT and had normal plasma biochemical features and liver morphology up to 60 days post-transplant. Additional studies are needed to determine whether FAH development constitutes a risk for recipients while waiting for whole organ transplant.
Fil: Vera, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Biancardi, Maria Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bottai, Hebe María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lugano, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Pisani, Gerardo Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quintana, Alejandra Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
FOCI OF ALTERED HEPATOCYTES
HEPATOCARCINOGENESIS
HEPATOCYTE TRANSPLANTATION
MARGINAL DONORS
RAT LIVER PRENEOPLASIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120613
Acceder
id CONICETDig_136acffca968db8648cbb4c7047dcc8f
oai_identifier_str oai:ri.conicet.gov.ar:11336/120613
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipientsVera, Marina CeciliaBiancardi, Maria EugeniaBottai, Hebe MaríaLugano, Maria CristinaQuiroga, Ariel DarioPisani, Gerardo BrunoQuintana, Alejandra BeatrizFOCI OF ALTERED HEPATOCYTESHEPATOCARCINOGENESISHEPATOCYTE TRANSPLANTATIONMARGINAL DONORSRAT LIVER PRENEOPLASIAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: the shortage of donors of hepatocyte transplantation therapy led to the use of so-called marginal donors. Some donors may have a hepatic illnesses that is associated with hepatic preneoplasia with foci of altered hepatocytes (FAH). Aims: to determine whether recipients developed FAH upon transplantation with hepatocytes from a preneoplastic liver and whether FAH progresses to a preneoplastic hepatocyte- derived tumor (PHDT), up to 60 days after transplantation. Material and methods: male Wistar adult rats were used as donors and recipients. Donors underwent a 2-phase model of liver preneoplasia for hepatocyte isolation. Recipients underwent a partial two thirds hepatectomy and received 150,000 hepatocytes. Recipients were euthanized seven and 60 days after transplantation. The number of FAH per liver area, percentage of liver occupied by FAH, the hepatic enzymatic profile, the percentage of prothrombin time (PT), the proliferative index (PI) and liver morphology were analyzed. Results: recipients developed few and very isolated FAH. No statistical differences were found between hepatic enzyme activities and PT. There were no differences between the groups with regard to the number of FAH per liver area and percentage of liver occupied by FAH after 60 days. The PI decreased on day 60 compared to day seven. No morphological alterations were found. Conclusions: recipients developed few FAH that did not increase in number or size, nor did they progress to PHDT and had normal plasma biochemical features and liver morphology up to 60 days post-transplant. Additional studies are needed to determine whether FAH development constitutes a risk for recipients while waiting for whole organ transplant.Fil: Vera, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; ArgentinaFil: Biancardi, Maria Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Bottai, Hebe María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lugano, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; ArgentinaFil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; ArgentinaFil: Pisani, Gerardo Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Quintana, Alejandra Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaAran Ediciones2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120613Vera, Marina Cecilia; Biancardi, Maria Eugenia; Bottai, Hebe María; Lugano, Maria Cristina; Quiroga, Ariel Dario; et al.; First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients; Aran Ediciones; Revista Española de Enfermedades Digestivas; 111; 5; 5-2019; 351-3571130-0108CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.17235/reed.2019.5830/2018info:eu-repo/semantics/altIdentifier/url/https://www.reed.es/first-approaches-for-the-transplantation-of-hepatocytes-from-wistar-rat-preneoplastic-livers-into-healthy-recipients3561info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:51Zoai:ri.conicet.gov.ar:11336/120613instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:52.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
title First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
spellingShingle First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
Vera, Marina Cecilia
FOCI OF ALTERED HEPATOCYTES
HEPATOCARCINOGENESIS
HEPATOCYTE TRANSPLANTATION
MARGINAL DONORS
RAT LIVER PRENEOPLASIA
title_short First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
title_full First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
title_fullStr First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
title_full_unstemmed First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
title_sort First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients
dc.creator.none.fl_str_mv Vera, Marina Cecilia
Biancardi, Maria Eugenia
Bottai, Hebe María
Lugano, Maria Cristina
Quiroga, Ariel Dario
Pisani, Gerardo Bruno
Quintana, Alejandra Beatriz
author Vera, Marina Cecilia
author_facet Vera, Marina Cecilia
Biancardi, Maria Eugenia
Bottai, Hebe María
Lugano, Maria Cristina
Quiroga, Ariel Dario
Pisani, Gerardo Bruno
Quintana, Alejandra Beatriz
author_role author
author2 Biancardi, Maria Eugenia
Bottai, Hebe María
Lugano, Maria Cristina
Quiroga, Ariel Dario
Pisani, Gerardo Bruno
Quintana, Alejandra Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv FOCI OF ALTERED HEPATOCYTES
HEPATOCARCINOGENESIS
HEPATOCYTE TRANSPLANTATION
MARGINAL DONORS
RAT LIVER PRENEOPLASIA
topic FOCI OF ALTERED HEPATOCYTES
HEPATOCARCINOGENESIS
HEPATOCYTE TRANSPLANTATION
MARGINAL DONORS
RAT LIVER PRENEOPLASIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: the shortage of donors of hepatocyte transplantation therapy led to the use of so-called marginal donors. Some donors may have a hepatic illnesses that is associated with hepatic preneoplasia with foci of altered hepatocytes (FAH). Aims: to determine whether recipients developed FAH upon transplantation with hepatocytes from a preneoplastic liver and whether FAH progresses to a preneoplastic hepatocyte- derived tumor (PHDT), up to 60 days after transplantation. Material and methods: male Wistar adult rats were used as donors and recipients. Donors underwent a 2-phase model of liver preneoplasia for hepatocyte isolation. Recipients underwent a partial two thirds hepatectomy and received 150,000 hepatocytes. Recipients were euthanized seven and 60 days after transplantation. The number of FAH per liver area, percentage of liver occupied by FAH, the hepatic enzymatic profile, the percentage of prothrombin time (PT), the proliferative index (PI) and liver morphology were analyzed. Results: recipients developed few and very isolated FAH. No statistical differences were found between hepatic enzyme activities and PT. There were no differences between the groups with regard to the number of FAH per liver area and percentage of liver occupied by FAH after 60 days. The PI decreased on day 60 compared to day seven. No morphological alterations were found. Conclusions: recipients developed few FAH that did not increase in number or size, nor did they progress to PHDT and had normal plasma biochemical features and liver morphology up to 60 days post-transplant. Additional studies are needed to determine whether FAH development constitutes a risk for recipients while waiting for whole organ transplant.
Fil: Vera, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Biancardi, Maria Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bottai, Hebe María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lugano, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Quiroga, Ariel Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Fisiología Experimental. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental; Argentina
Fil: Pisani, Gerardo Bruno. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Quintana, Alejandra Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Background: the shortage of donors of hepatocyte transplantation therapy led to the use of so-called marginal donors. Some donors may have a hepatic illnesses that is associated with hepatic preneoplasia with foci of altered hepatocytes (FAH). Aims: to determine whether recipients developed FAH upon transplantation with hepatocytes from a preneoplastic liver and whether FAH progresses to a preneoplastic hepatocyte- derived tumor (PHDT), up to 60 days after transplantation. Material and methods: male Wistar adult rats were used as donors and recipients. Donors underwent a 2-phase model of liver preneoplasia for hepatocyte isolation. Recipients underwent a partial two thirds hepatectomy and received 150,000 hepatocytes. Recipients were euthanized seven and 60 days after transplantation. The number of FAH per liver area, percentage of liver occupied by FAH, the hepatic enzymatic profile, the percentage of prothrombin time (PT), the proliferative index (PI) and liver morphology were analyzed. Results: recipients developed few and very isolated FAH. No statistical differences were found between hepatic enzyme activities and PT. There were no differences between the groups with regard to the number of FAH per liver area and percentage of liver occupied by FAH after 60 days. The PI decreased on day 60 compared to day seven. No morphological alterations were found. Conclusions: recipients developed few FAH that did not increase in number or size, nor did they progress to PHDT and had normal plasma biochemical features and liver morphology up to 60 days post-transplant. Additional studies are needed to determine whether FAH development constitutes a risk for recipients while waiting for whole organ transplant.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120613
Vera, Marina Cecilia; Biancardi, Maria Eugenia; Bottai, Hebe María; Lugano, Maria Cristina; Quiroga, Ariel Dario; et al.; First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients; Aran Ediciones; Revista Española de Enfermedades Digestivas; 111; 5; 5-2019; 351-357
1130-0108
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120613
identifier_str_mv Vera, Marina Cecilia; Biancardi, Maria Eugenia; Bottai, Hebe María; Lugano, Maria Cristina; Quiroga, Ariel Dario; et al.; First approaches for the transplantation of hepatocytes from Wistar rat preneoplastic livers into healthy recipients; Aran Ediciones; Revista Española de Enfermedades Digestivas; 111; 5; 5-2019; 351-357
1130-0108
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.17235/reed.2019.5830/2018
info:eu-repo/semantics/altIdentifier/url/https://www.reed.es/first-approaches-for-the-transplantation-of-hepatocytes-from-wistar-rat-preneoplastic-livers-into-healthy-recipients3561
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Aran Ediciones
publisher.none.fl_str_mv Aran Ediciones
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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