Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability
- Autores
- Serantes, Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefts, there have been no recent signifcant advances in enhancing their efcacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, eforts have focused on selecting spontaneous mutations in genes associated with denitrifcation and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system ofers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants.
Fil: Serantes, Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Stritzler, Margarita. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina - Materia
-
EDICION GÉNICA
BRADYRHIZOBIUM
CRISPR
INOCULANTE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/244050
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Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainabilitySerantes, LauraStritzler, MargaritaBrambilla, Silvina MaricelSoto, Gabriela CynthiaAyub, Nicolás DanielEDICION GÉNICABRADYRHIZOBIUMCRISPRINOCULANTEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefts, there have been no recent signifcant advances in enhancing their efcacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, eforts have focused on selecting spontaneous mutations in genes associated with denitrifcation and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system ofers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants.Fil: Serantes, Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Stritzler, Margarita. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaSpringer2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244050Serantes, Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability; Springer; Plant Cell, Tissue and Organ Culture; 157; 2; 4-2024; 1-40167-6857CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11240-024-02764-yinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-024-02764-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:12Zoai:ri.conicet.gov.ar:11336/244050instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:12.64CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
title |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
spellingShingle |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability Serantes, Laura EDICION GÉNICA BRADYRHIZOBIUM CRISPR INOCULANTE |
title_short |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
title_full |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
title_fullStr |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
title_full_unstemmed |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
title_sort |
Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability |
dc.creator.none.fl_str_mv |
Serantes, Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author |
Serantes, Laura |
author_facet |
Serantes, Laura Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author_role |
author |
author2 |
Stritzler, Margarita Brambilla, Silvina Maricel Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
EDICION GÉNICA BRADYRHIZOBIUM CRISPR INOCULANTE |
topic |
EDICION GÉNICA BRADYRHIZOBIUM CRISPR INOCULANTE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefts, there have been no recent signifcant advances in enhancing their efcacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, eforts have focused on selecting spontaneous mutations in genes associated with denitrifcation and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system ofers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants. Fil: Serantes, Laura. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina |
description |
In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while reducing reliance on agrochemicals. Despite their benefts, there have been no recent signifcant advances in enhancing their efcacy. Notably, Bradyrhizobium japonicum E109 and Bradyrhizobium japonicum SEMIA 5079, the most globally utilized, were isolated decades ago, underscoring the need for genetic enhancement. Recently, eforts have focused on selecting spontaneous mutations in genes associated with denitrifcation and oxidative stress. While this technology has shown promise in reducing nitrous oxide emissions and enhancing root colonization, selecting mutants remains costly and challenging without clear phenotypic markers. The CRISPR/Cas9 system ofers a potential solution, although validation is limited to model strains such as Sinorhizobium meliloti 1021. Using the CRISPR/Cas9 system and three sgRNAs, we have edited the genes 16S, napA and glxA from strains E109 and SEMIA5079, generating mutants with streptomycin tolerance, reduced nitrate reductase activity, and increased catalase activity, respectively. Finally, we discuss how the CRISPR/Cas9 system can contribute to bridging the gap between crop and inoculant improvement, and its possible role in producing a new generation of climate-smart inoculants. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/244050 Serantes, Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability; Springer; Plant Cell, Tissue and Organ Culture; 157; 2; 4-2024; 1-4 0167-6857 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/244050 |
identifier_str_mv |
Serantes, Laura; Stritzler, Margarita; Brambilla, Silvina Maricel; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel; Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability; Springer; Plant Cell, Tissue and Organ Culture; 157; 2; 4-2024; 1-4 0167-6857 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11240-024-02764-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-024-02764-y |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |