Conformational changes on substrate binding revealed by structures of Methylobacterium extorquens malate dehydrogenase

• Autores: Gonzalez, Javier Marcelo; Martí Arbona, Ricardo; Chen, Julian; Broom Peltz, Brian; Unkefer, Clifford
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: BIOFUELS; MALATE DEHYDROGENASE; METHYLOBACTERIUM EXTORQUENS; METHYLOTROPHS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Three high-resolution X-ray crystal structures of malate dehydrogenase (MDH; EC 1.1.1.37) from the methylotroph Methylobacterium extorquens AM1 are presented. By comparing the structures of apo MDH, a binary complex of MDH and NAD+, and a ternary complex of MDH and oxaloacetate with ADP-ribose occupying the pyridine nucleotide-binding site, conformational changes associated with the formation of the catalytic complex were characterized. While the substrate-binding site is accessible in the enzyme resting state or NAD+-bound forms, the substrate-bound form exhibits a closed conformation. This conformational change involves the transition of an α-helix to a 310-helix, which causes the adjacent loop to close the active site following coenzyme and substrate binding. In the ternary complex, His284 forms a hydrogen bond to the C2 carbonyl of oxaloacetate, placing it in a position to donate a proton in the formation of (2S)-malate.Crystal structures of apo malate dehydrogenase (MDH) from Methylobacterium extorquens, MDH bound to NAD+, and MDH with oxaloacetate and ADP-ribose revealed conformational changes, closing the active site upon coenzyme and substrate binding. In the ternary complex, His284 is in position to donate a proton in the formation of (2S)-malate.
Fil: Gonzalez, Javier Marcelo. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del Noa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Bionanotecnología del Noa; Argentina
Fil: Marti Arbona, R.. Bioscience Division, Los Alamos National Laboratory; Estados Unidos
Fil: Chen, J. C. H.. Bioscience Division, Los Alamos National Laboratory; Estados Unidos
Fil: Broom Peltz, B.. Bioscience Division, Los Alamos National Laboratory; Estados Unidos
Fil: Unkefer, C. J.. Bioscience Division, Los Alamos National Laboratory; Estados Unidos