Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation

Autores
Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.
Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina
Materia
Cyclooxigenase
Lipooxigenase
Oocyte Maturation
Phospholipase A2
Rhinella Arenarum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20540

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network_name_str CONICET Digital (CONICET)
spelling Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturationOrtiz, María EugeniaBühler, Marta InesZelarayán, Liliana IsabelCyclooxigenaseLipooxigenaseOocyte MaturationPhospholipase A2Rhinella Arenarumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; ArgentinaCambridge University Press2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20540Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-4450967-19941469-8730CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/involvement-of-pla2-cox-and-lox-in-rhinella-arenarum-oocyte-maturation/82FB8AF0C94B4D3110A4A9D1683FA998info:eu-repo/semantics/altIdentifier/doi/10.1017/S096719941200069Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:16Zoai:ri.conicet.gov.ar:11336/20540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:17.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
title Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
spellingShingle Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
Ortiz, María Eugenia
Cyclooxigenase
Lipooxigenase
Oocyte Maturation
Phospholipase A2
Rhinella Arenarum
title_short Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
title_full Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
title_fullStr Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
title_full_unstemmed Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
title_sort Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
dc.creator.none.fl_str_mv Ortiz, María Eugenia
Bühler, Marta Ines
Zelarayán, Liliana Isabel
author Ortiz, María Eugenia
author_facet Ortiz, María Eugenia
Bühler, Marta Ines
Zelarayán, Liliana Isabel
author_role author
author2 Bühler, Marta Ines
Zelarayán, Liliana Isabel
author2_role author
author
dc.subject.none.fl_str_mv Cyclooxigenase
Lipooxigenase
Oocyte Maturation
Phospholipase A2
Rhinella Arenarum
topic Cyclooxigenase
Lipooxigenase
Oocyte Maturation
Phospholipase A2
Rhinella Arenarum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.
Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina
description In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20540
Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-445
0967-1994
1469-8730
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20540
identifier_str_mv Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-445
0967-1994
1469-8730
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/involvement-of-pla2-cox-and-lox-in-rhinella-arenarum-oocyte-maturation/82FB8AF0C94B4D3110A4A9D1683FA998
info:eu-repo/semantics/altIdentifier/doi/10.1017/S096719941200069X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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