Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
- Autores
- Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.
Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina - Materia
-
Cyclooxigenase
Lipooxigenase
Oocyte Maturation
Phospholipase A2
Rhinella Arenarum - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/20540
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Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturationOrtiz, María EugeniaBühler, Marta InesZelarayán, Liliana IsabelCyclooxigenaseLipooxigenaseOocyte MaturationPhospholipase A2Rhinella Arenarumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; ArgentinaCambridge University Press2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20540Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-4450967-19941469-8730CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/involvement-of-pla2-cox-and-lox-in-rhinella-arenarum-oocyte-maturation/82FB8AF0C94B4D3110A4A9D1683FA998info:eu-repo/semantics/altIdentifier/doi/10.1017/S096719941200069Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:16Zoai:ri.conicet.gov.ar:11336/20540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:17.067CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
title |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
spellingShingle |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation Ortiz, María Eugenia Cyclooxigenase Lipooxigenase Oocyte Maturation Phospholipase A2 Rhinella Arenarum |
title_short |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
title_full |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
title_fullStr |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
title_full_unstemmed |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
title_sort |
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation |
dc.creator.none.fl_str_mv |
Ortiz, María Eugenia Bühler, Marta Ines Zelarayán, Liliana Isabel |
author |
Ortiz, María Eugenia |
author_facet |
Ortiz, María Eugenia Bühler, Marta Ines Zelarayán, Liliana Isabel |
author_role |
author |
author2 |
Bühler, Marta Ines Zelarayán, Liliana Isabel |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Cyclooxigenase Lipooxigenase Oocyte Maturation Phospholipase A2 Rhinella Arenarum |
topic |
Cyclooxigenase Lipooxigenase Oocyte Maturation Phospholipase A2 Rhinella Arenarum |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation. Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina |
description |
In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/20540 Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-445 0967-1994 1469-8730 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/20540 |
identifier_str_mv |
Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-445 0967-1994 1469-8730 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/involvement-of-pla2-cox-and-lox-in-rhinella-arenarum-oocyte-maturation/82FB8AF0C94B4D3110A4A9D1683FA998 info:eu-repo/semantics/altIdentifier/doi/10.1017/S096719941200069X |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |