Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides

Autores
Achimón, Fernanda; Dambolena, José Sebastián; Zygadlo, Julio Alberto; Pizzolitto, Romina Paola
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium verticillioidesis a fungal pathogen of maize and a prolific producer of fumonisin B1(FB1), which has encouraged the search of analytical methods for FB1 detection during grain storage.Secondary metabolites(FB1 and sesquiterpenes) are synthetized from precursors derived from primary metabolism, which is strongly affected by carbon sources. The purpose of our study was to evaluate the effect of different carbohydrates on vegetative growth, conidiation and secondary metabolism in F. verticillioides. We observed lower values of lag period in cultures with amylopectin, amylose and starch (0.06 ± 0.01, 0.18 ± 0.01 and 0.26 ± 0.00 day,respectively), along with elongated hyphae. Besides, amylose and maltose stimulated fungal growth(5.50 ± 0.01 and 5.23 ± 0.01 mm/day, respectively) and conidiation (13.75×105± 0.58×105 and 13.49×105± 0.38×105conidia/ml).Furthermore,maximum production of FB1 was achieved with glucose(4.4×103±0.18×103μg/g DW), while sesquiterpene production was higher with amylopectin(158.5×10−12± 8.80×10−12μg/g DW), highlighting the complex mechanisms that regulate fungal secondary metabolism. Finally, we proposed a sesquiterpene profile that couldbe used as a volatile biomarker of FB1 contamination in stored maize kernels.
Fil: Achimón, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Pizzolitto, Romina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
FUSARIUM VERTICILLIOIDES
CARBON SOURCES
VEGETATIVE GROWTH
SECONDARY METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111074
Acceder
id CONICETDig_12c105803494d96e4bdd063998b6ad1d
oai_identifier_str oai:ri.conicet.gov.ar:11336/111074
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioidesAchimón, FernandaDambolena, José SebastiánZygadlo, Julio AlbertoPizzolitto, Romina PaolaFUSARIUM VERTICILLIOIDESCARBON SOURCESVEGETATIVE GROWTHSECONDARY METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fusarium verticillioidesis a fungal pathogen of maize and a prolific producer of fumonisin B1(FB1), which has encouraged the search of analytical methods for FB1 detection during grain storage.Secondary metabolites(FB1 and sesquiterpenes) are synthetized from precursors derived from primary metabolism, which is strongly affected by carbon sources. The purpose of our study was to evaluate the effect of different carbohydrates on vegetative growth, conidiation and secondary metabolism in F. verticillioides. We observed lower values of lag period in cultures with amylopectin, amylose and starch (0.06 ± 0.01, 0.18 ± 0.01 and 0.26 ± 0.00 day,respectively), along with elongated hyphae. Besides, amylose and maltose stimulated fungal growth(5.50 ± 0.01 and 5.23 ± 0.01 mm/day, respectively) and conidiation (13.75×105± 0.58×105 and 13.49×105± 0.38×105conidia/ml).Furthermore,maximum production of FB1 was achieved with glucose(4.4×103±0.18×103μg/g DW), while sesquiterpene production was higher with amylopectin(158.5×10−12± 8.80×10−12μg/g DW), highlighting the complex mechanisms that regulate fungal secondary metabolism. Finally, we proposed a sesquiterpene profile that couldbe used as a volatile biomarker of FB1 contamination in stored maize kernels.Fil: Achimón, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pizzolitto, Romina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaElsevier Science2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111074Achimón, Fernanda; Dambolena, José Sebastián; Zygadlo, Julio Alberto; Pizzolitto, Romina Paola; Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides; Elsevier Science; LWT - Food Science and Technology; 115; 11-20190023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819308126info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108470info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:10Zoai:ri.conicet.gov.ar:11336/111074instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:10.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
title Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
spellingShingle Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
Achimón, Fernanda
FUSARIUM VERTICILLIOIDES
CARBON SOURCES
VEGETATIVE GROWTH
SECONDARY METABOLISM
title_short Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
title_full Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
title_fullStr Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
title_full_unstemmed Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
title_sort Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides
dc.creator.none.fl_str_mv Achimón, Fernanda
Dambolena, José Sebastián
Zygadlo, Julio Alberto
Pizzolitto, Romina Paola
author Achimón, Fernanda
author_facet Achimón, Fernanda
Dambolena, José Sebastián
Zygadlo, Julio Alberto
Pizzolitto, Romina Paola
author_role author
author2 Dambolena, José Sebastián
Zygadlo, Julio Alberto
Pizzolitto, Romina Paola
author2_role author
author
author
dc.subject.none.fl_str_mv FUSARIUM VERTICILLIOIDES
CARBON SOURCES
VEGETATIVE GROWTH
SECONDARY METABOLISM
topic FUSARIUM VERTICILLIOIDES
CARBON SOURCES
VEGETATIVE GROWTH
SECONDARY METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fusarium verticillioidesis a fungal pathogen of maize and a prolific producer of fumonisin B1(FB1), which has encouraged the search of analytical methods for FB1 detection during grain storage.Secondary metabolites(FB1 and sesquiterpenes) are synthetized from precursors derived from primary metabolism, which is strongly affected by carbon sources. The purpose of our study was to evaluate the effect of different carbohydrates on vegetative growth, conidiation and secondary metabolism in F. verticillioides. We observed lower values of lag period in cultures with amylopectin, amylose and starch (0.06 ± 0.01, 0.18 ± 0.01 and 0.26 ± 0.00 day,respectively), along with elongated hyphae. Besides, amylose and maltose stimulated fungal growth(5.50 ± 0.01 and 5.23 ± 0.01 mm/day, respectively) and conidiation (13.75×105± 0.58×105 and 13.49×105± 0.38×105conidia/ml).Furthermore,maximum production of FB1 was achieved with glucose(4.4×103±0.18×103μg/g DW), while sesquiterpene production was higher with amylopectin(158.5×10−12± 8.80×10−12μg/g DW), highlighting the complex mechanisms that regulate fungal secondary metabolism. Finally, we proposed a sesquiterpene profile that couldbe used as a volatile biomarker of FB1 contamination in stored maize kernels.
Fil: Achimón, Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Zygadlo, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Pizzolitto, Romina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Fusarium verticillioidesis a fungal pathogen of maize and a prolific producer of fumonisin B1(FB1), which has encouraged the search of analytical methods for FB1 detection during grain storage.Secondary metabolites(FB1 and sesquiterpenes) are synthetized from precursors derived from primary metabolism, which is strongly affected by carbon sources. The purpose of our study was to evaluate the effect of different carbohydrates on vegetative growth, conidiation and secondary metabolism in F. verticillioides. We observed lower values of lag period in cultures with amylopectin, amylose and starch (0.06 ± 0.01, 0.18 ± 0.01 and 0.26 ± 0.00 day,respectively), along with elongated hyphae. Besides, amylose and maltose stimulated fungal growth(5.50 ± 0.01 and 5.23 ± 0.01 mm/day, respectively) and conidiation (13.75×105± 0.58×105 and 13.49×105± 0.38×105conidia/ml).Furthermore,maximum production of FB1 was achieved with glucose(4.4×103±0.18×103μg/g DW), while sesquiterpene production was higher with amylopectin(158.5×10−12± 8.80×10−12μg/g DW), highlighting the complex mechanisms that regulate fungal secondary metabolism. Finally, we proposed a sesquiterpene profile that couldbe used as a volatile biomarker of FB1 contamination in stored maize kernels.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111074
Achimón, Fernanda; Dambolena, José Sebastián; Zygadlo, Julio Alberto; Pizzolitto, Romina Paola; Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides; Elsevier Science; LWT - Food Science and Technology; 115; 11-2019
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111074
identifier_str_mv Achimón, Fernanda; Dambolena, José Sebastián; Zygadlo, Julio Alberto; Pizzolitto, Romina Paola; Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides; Elsevier Science; LWT - Food Science and Technology; 115; 11-2019
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819308126
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108470
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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