Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling

Autores
Taher, Shèdy; Borja, Yamilette; Cabanela, Lucía; Costers, Vincent J.; Carson Marino, Morgan; Bailes, Julie C.; Dhar, Biswadeep; Beckworth, Mark T.; Rabaglino, Maria Belen; Post Uiterweer, Emiel D.; Conrad, Kirk P.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We investigated expression of cholecystokinin (CCK) in humans and mice, and the bitter taste receptor TAS2R14 in the human placenta. Because CCK and gastrin activate the CCKBR receptor, we also explored placental gastrin expression. Finally, we investigated calcium signaling by CCK and TAS2R14. By RT-PCR, we found CCK/Cck and GAST/Gast mRNA expression in both normal human and mouse placentas, as well as in human trophoblast cell lines (TCL). Although both Cckar and -br mRNA were expressed in the mouse placenta, only CCKBR mRNA was detected in the human placenta and TCL. mRNA expression for TAS2R14 was also observed in the human placenta and TCL. Using immunohistochemistry, CCK protein was localized to the syncytiotrophoblast (ST) and extravillous trophoblast (EVT) in the human term placenta, and to trophoblast glycogen cells in mouse and human placentas. Gastrin and TAS2R14 proteins were also observed in ST and EVT of the human placenta. Both sulfated and nonsulfated CCK elicited a comparable rise in intracellular calcium in TCL, consistent with CCKBR expression. Three TAS2R14 agonists, flufenamic acid, chlorhexidine, and diphenhydramine, also evoked rises in intracellular calcium in TCL. These results establish CCK, gastrin, and their receptor(s) in both human and mouse placentas, and TAS2R14 in the human placenta. Both CCK and TAS2R14 agonists increased intracellular calcium in human TCL. Although the roles of these ligands and receptors, and their potential cross talk in normal and pathological placentas, are currently unknown, this study opens new avenues for placental research.
Fil: Taher, Shèdy. University of Florida; Estados Unidos
Fil: Borja, Yamilette. University of Florida; Estados Unidos
Fil: Cabanela, Lucía. University of Florida; Estados Unidos
Fil: Costers, Vincent J.. University of Florida; Estados Unidos
Fil: Carson Marino, Morgan. University of Florida; Estados Unidos
Fil: Bailes, Julie C.. University of Florida; Estados Unidos
Fil: Dhar, Biswadeep. University of Florida; Estados Unidos
Fil: Beckworth, Mark T.. University of Florida; Estados Unidos
Fil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Post Uiterweer, Emiel D.. University Medical Center Utrecht; Países Bajos
Fil: Conrad, Kirk P.. University of Florida; Estados Unidos
Materia
EXTRAVILLOUS TROPHOBLAST
INTRACELLULAR CALCIUM
PLACENTA
SYNCYTIOTROPHOBLAST
TROPHOBLAST GLYCOGEN CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/125587
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/125587
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signalingTaher, ShèdyBorja, YamiletteCabanela, LucíaCosters, Vincent J.Carson Marino, MorganBailes, Julie C.Dhar, BiswadeepBeckworth, Mark T.Rabaglino, Maria BelenPost Uiterweer, Emiel D.Conrad, Kirk P.EXTRAVILLOUS TROPHOBLASTINTRACELLULAR CALCIUMPLACENTASYNCYTIOTROPHOBLASTTROPHOBLAST GLYCOGEN CELLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We investigated expression of cholecystokinin (CCK) in humans and mice, and the bitter taste receptor TAS2R14 in the human placenta. Because CCK and gastrin activate the CCKBR receptor, we also explored placental gastrin expression. Finally, we investigated calcium signaling by CCK and TAS2R14. By RT-PCR, we found CCK/Cck and GAST/Gast mRNA expression in both normal human and mouse placentas, as well as in human trophoblast cell lines (TCL). Although both Cckar and -br mRNA were expressed in the mouse placenta, only CCKBR mRNA was detected in the human placenta and TCL. mRNA expression for TAS2R14 was also observed in the human placenta and TCL. Using immunohistochemistry, CCK protein was localized to the syncytiotrophoblast (ST) and extravillous trophoblast (EVT) in the human term placenta, and to trophoblast glycogen cells in mouse and human placentas. Gastrin and TAS2R14 proteins were also observed in ST and EVT of the human placenta. Both sulfated and nonsulfated CCK elicited a comparable rise in intracellular calcium in TCL, consistent with CCKBR expression. Three TAS2R14 agonists, flufenamic acid, chlorhexidine, and diphenhydramine, also evoked rises in intracellular calcium in TCL. These results establish CCK, gastrin, and their receptor(s) in both human and mouse placentas, and TAS2R14 in the human placenta. Both CCK and TAS2R14 agonists increased intracellular calcium in human TCL. Although the roles of these ligands and receptors, and their potential cross talk in normal and pathological placentas, are currently unknown, this study opens new avenues for placental research.Fil: Taher, Shèdy. University of Florida; Estados UnidosFil: Borja, Yamilette. University of Florida; Estados UnidosFil: Cabanela, Lucía. University of Florida; Estados UnidosFil: Costers, Vincent J.. University of Florida; Estados UnidosFil: Carson Marino, Morgan. University of Florida; Estados UnidosFil: Bailes, Julie C.. University of Florida; Estados UnidosFil: Dhar, Biswadeep. University of Florida; Estados UnidosFil: Beckworth, Mark T.. University of Florida; Estados UnidosFil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Post Uiterweer, Emiel D.. University Medical Center Utrecht; Países BajosFil: Conrad, Kirk P.. University of Florida; Estados UnidosAmerican Physiology Society2019-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/125587Taher, Shèdy; Borja, Yamilette; Cabanela, Lucía; Costers, Vincent J.; Carson Marino, Morgan; et al.; Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling; American Physiology Society; American Journal of Physiology: Regulatory, Integrative and Comparative Physiology; 316; 5; 1-5-2019; 628-6391522-1490CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1152/ajpregu.00153.2018info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpregu.00153.2018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:01Zoai:ri.conicet.gov.ar:11336/125587instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:01.807CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
title Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
spellingShingle Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
Taher, Shèdy
EXTRAVILLOUS TROPHOBLAST
INTRACELLULAR CALCIUM
PLACENTA
SYNCYTIOTROPHOBLAST
TROPHOBLAST GLYCOGEN CELL
title_short Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
title_full Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
title_fullStr Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
title_full_unstemmed Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
title_sort Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling
dc.creator.none.fl_str_mv Taher, Shèdy
Borja, Yamilette
Cabanela, Lucía
Costers, Vincent J.
Carson Marino, Morgan
Bailes, Julie C.
Dhar, Biswadeep
Beckworth, Mark T.
Rabaglino, Maria Belen
Post Uiterweer, Emiel D.
Conrad, Kirk P.
author Taher, Shèdy
author_facet Taher, Shèdy
Borja, Yamilette
Cabanela, Lucía
Costers, Vincent J.
Carson Marino, Morgan
Bailes, Julie C.
Dhar, Biswadeep
Beckworth, Mark T.
Rabaglino, Maria Belen
Post Uiterweer, Emiel D.
Conrad, Kirk P.
author_role author
author2 Borja, Yamilette
Cabanela, Lucía
Costers, Vincent J.
Carson Marino, Morgan
Bailes, Julie C.
Dhar, Biswadeep
Beckworth, Mark T.
Rabaglino, Maria Belen
Post Uiterweer, Emiel D.
Conrad, Kirk P.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EXTRAVILLOUS TROPHOBLAST
INTRACELLULAR CALCIUM
PLACENTA
SYNCYTIOTROPHOBLAST
TROPHOBLAST GLYCOGEN CELL
topic EXTRAVILLOUS TROPHOBLAST
INTRACELLULAR CALCIUM
PLACENTA
SYNCYTIOTROPHOBLAST
TROPHOBLAST GLYCOGEN CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We investigated expression of cholecystokinin (CCK) in humans and mice, and the bitter taste receptor TAS2R14 in the human placenta. Because CCK and gastrin activate the CCKBR receptor, we also explored placental gastrin expression. Finally, we investigated calcium signaling by CCK and TAS2R14. By RT-PCR, we found CCK/Cck and GAST/Gast mRNA expression in both normal human and mouse placentas, as well as in human trophoblast cell lines (TCL). Although both Cckar and -br mRNA were expressed in the mouse placenta, only CCKBR mRNA was detected in the human placenta and TCL. mRNA expression for TAS2R14 was also observed in the human placenta and TCL. Using immunohistochemistry, CCK protein was localized to the syncytiotrophoblast (ST) and extravillous trophoblast (EVT) in the human term placenta, and to trophoblast glycogen cells in mouse and human placentas. Gastrin and TAS2R14 proteins were also observed in ST and EVT of the human placenta. Both sulfated and nonsulfated CCK elicited a comparable rise in intracellular calcium in TCL, consistent with CCKBR expression. Three TAS2R14 agonists, flufenamic acid, chlorhexidine, and diphenhydramine, also evoked rises in intracellular calcium in TCL. These results establish CCK, gastrin, and their receptor(s) in both human and mouse placentas, and TAS2R14 in the human placenta. Both CCK and TAS2R14 agonists increased intracellular calcium in human TCL. Although the roles of these ligands and receptors, and their potential cross talk in normal and pathological placentas, are currently unknown, this study opens new avenues for placental research.
Fil: Taher, Shèdy. University of Florida; Estados Unidos
Fil: Borja, Yamilette. University of Florida; Estados Unidos
Fil: Cabanela, Lucía. University of Florida; Estados Unidos
Fil: Costers, Vincent J.. University of Florida; Estados Unidos
Fil: Carson Marino, Morgan. University of Florida; Estados Unidos
Fil: Bailes, Julie C.. University of Florida; Estados Unidos
Fil: Dhar, Biswadeep. University of Florida; Estados Unidos
Fil: Beckworth, Mark T.. University of Florida; Estados Unidos
Fil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Post Uiterweer, Emiel D.. University Medical Center Utrecht; Países Bajos
Fil: Conrad, Kirk P.. University of Florida; Estados Unidos
description We investigated expression of cholecystokinin (CCK) in humans and mice, and the bitter taste receptor TAS2R14 in the human placenta. Because CCK and gastrin activate the CCKBR receptor, we also explored placental gastrin expression. Finally, we investigated calcium signaling by CCK and TAS2R14. By RT-PCR, we found CCK/Cck and GAST/Gast mRNA expression in both normal human and mouse placentas, as well as in human trophoblast cell lines (TCL). Although both Cckar and -br mRNA were expressed in the mouse placenta, only CCKBR mRNA was detected in the human placenta and TCL. mRNA expression for TAS2R14 was also observed in the human placenta and TCL. Using immunohistochemistry, CCK protein was localized to the syncytiotrophoblast (ST) and extravillous trophoblast (EVT) in the human term placenta, and to trophoblast glycogen cells in mouse and human placentas. Gastrin and TAS2R14 proteins were also observed in ST and EVT of the human placenta. Both sulfated and nonsulfated CCK elicited a comparable rise in intracellular calcium in TCL, consistent with CCKBR expression. Three TAS2R14 agonists, flufenamic acid, chlorhexidine, and diphenhydramine, also evoked rises in intracellular calcium in TCL. These results establish CCK, gastrin, and their receptor(s) in both human and mouse placentas, and TAS2R14 in the human placenta. Both CCK and TAS2R14 agonists increased intracellular calcium in human TCL. Although the roles of these ligands and receptors, and their potential cross talk in normal and pathological placentas, are currently unknown, this study opens new avenues for placental research.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/125587
Taher, Shèdy; Borja, Yamilette; Cabanela, Lucía; Costers, Vincent J.; Carson Marino, Morgan; et al.; Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling; American Physiology Society; American Journal of Physiology: Regulatory, Integrative and Comparative Physiology; 316; 5; 1-5-2019; 628-639
1522-1490
CONICET Digital
CONICET
url http://hdl.handle.net/11336/125587
identifier_str_mv Taher, Shèdy; Borja, Yamilette; Cabanela, Lucía; Costers, Vincent J.; Carson Marino, Morgan; et al.; Cholecystokinin, gastrin, cholecystokinin/gastrin receptors, and bitter taste receptor TAS2R14: trophoblast expression and signaling; American Physiology Society; American Journal of Physiology: Regulatory, Integrative and Comparative Physiology; 316; 5; 1-5-2019; 628-639
1522-1490
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpregu.00153.2018
info:eu-repo/semantics/altIdentifier/url/https://journals.physiology.org/doi/full/10.1152/ajpregu.00153.2018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiology Society
publisher.none.fl_str_mv American Physiology Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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