Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
- Autores
- Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.
Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina - Materia
-
Virus-Free Plants
Lilium
Scaling
Thermotherapy
Meristem Tip Culture
Chemotherapy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/25291
Ver los metadatos del registro completo
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network_name_str |
CONICET Digital (CONICET) |
spelling |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitroChinestra, Silvia CarolinaCurvetto, Nestor RaulMarinangeli, Pablo AlejandroVirus-Free PlantsLiliumScalingThermotherapyMeristem Tip CultureChemotherapyhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaElsevier Science2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25291Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-3120304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.08.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815301345info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:05Zoai:ri.conicet.gov.ar:11336/25291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:05.438CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
title |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
spellingShingle |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro Chinestra, Silvia Carolina Virus-Free Plants Lilium Scaling Thermotherapy Meristem Tip Culture Chemotherapy |
title_short |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
title_full |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
title_fullStr |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
title_full_unstemmed |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
title_sort |
Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro |
dc.creator.none.fl_str_mv |
Chinestra, Silvia Carolina Curvetto, Nestor Raul Marinangeli, Pablo Alejandro |
author |
Chinestra, Silvia Carolina |
author_facet |
Chinestra, Silvia Carolina Curvetto, Nestor Raul Marinangeli, Pablo Alejandro |
author_role |
author |
author2 |
Curvetto, Nestor Raul Marinangeli, Pablo Alejandro |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Virus-Free Plants Lilium Scaling Thermotherapy Meristem Tip Culture Chemotherapy |
topic |
Virus-Free Plants Lilium Scaling Thermotherapy Meristem Tip Culture Chemotherapy |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations. Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina |
description |
Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/25291 Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-312 0304-4238 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/25291 |
identifier_str_mv |
Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-312 0304-4238 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.08.015 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815301345 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269675440832512 |
score |
13.13397 |