Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro

Autores
Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.
Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Virus-Free Plants
Lilium
Scaling
Thermotherapy
Meristem Tip Culture
Chemotherapy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25291
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25291
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitroChinestra, Silvia CarolinaCurvetto, Nestor RaulMarinangeli, Pablo AlejandroVirus-Free PlantsLiliumScalingThermotherapyMeristem Tip CultureChemotherapyhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaElsevier Science2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25291Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-3120304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.08.015info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815301345info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:58Zoai:ri.conicet.gov.ar:11336/25291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:59.057CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
title Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
spellingShingle Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
Chinestra, Silvia Carolina
Virus-Free Plants
Lilium
Scaling
Thermotherapy
Meristem Tip Culture
Chemotherapy
title_short Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
title_full Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
title_fullStr Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
title_full_unstemmed Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
title_sort Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro
dc.creator.none.fl_str_mv Chinestra, Silvia Carolina
Curvetto, Nestor Raul
Marinangeli, Pablo Alejandro
author Chinestra, Silvia Carolina
author_facet Chinestra, Silvia Carolina
Curvetto, Nestor Raul
Marinangeli, Pablo Alejandro
author_role author
author2 Curvetto, Nestor Raul
Marinangeli, Pablo Alejandro
author2_role author
author
dc.subject.none.fl_str_mv Virus-Free Plants
Lilium
Scaling
Thermotherapy
Meristem Tip Culture
Chemotherapy
topic Virus-Free Plants
Lilium
Scaling
Thermotherapy
Meristem Tip Culture
Chemotherapy
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.
Fil: Chinestra, Silvia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marinangeli, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Lilium tissue culture is used for mass propagation of elite material as well as for obtaining virus-free plants. The aim of the present study was to evaluate the effectiveness of meristem tip culture from microbulbs obtained in vitro and from bulblets obtained via scaling macropropagation – either with or without thermotherapy and chemotherapy treatments applied before meristem tip culture – to obtain virus-free Lilium spp. plants. To this end, microshoot regeneration from meristematic tips was first tested and two diagnostic techniques were compared after meristem tip culture treatment. Different alternatives were assayed in several Lilium hybrids to obtain virus-free plants. Effective virus elimination was possible using meristematic tips extracted from bulblets produced ex vitro by scaling, a procedure that has not been previously reported in Lilium. The number of virus-free plants obtained, which depended on the genotype as well as on the virus present in the original material, reached ∼100% via meristem tip culture with or without pre-thermotherapy treatment at 35 °C. Meristem tip culture produced 100% of LMoV-free plants in Lilium longiflorum ‘Snow Queen’ and LA hybrid ‘Lacorno’, also CMV-free plants in Asiatic hybrid ‘Navona’ and LA hybrid ‘Fangio’, and LSV-free plants in LA ‘Royal Respect’. The LSV infection rate decreased in Asiatic hybrid ‘Visconti’ when thermotherapy was applied ex vitro before meristem tip culture. Chemotherapy applied during in vitro bulb differentiation prior to meristem tip culture led to a complete elimination of LSV in the LO hybrid ‘Triumphator’. Ex vitro chemotherapy was ineffective in virus elimination even when applied at high concentrations.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25291
Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-312
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25291
identifier_str_mv Chinestra, Silvia Carolina; Curvetto, Nestor Raul; Marinangeli, Pablo Alejandro; Production of virus-free plants of Lilium spp. from bulbs obtained in vitro and ex vitro; Elsevier Science; Scientia Horticulturae; 194; 10-2015; 304-312
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.08.015
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815301345
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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