A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma

Autores
Piñero, Federico; Vazquez, Martín; Baré, Patricia; Rohr, Cristian Oscar; Mendizabal, Manuel; Sciara, Mariela Ines; Alonso, Cristina; Fay, Fabián; Silva, Marcelo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction and aim: A pro-oncogenic intestinal microbiome was observed in murine models; however, no specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported. We aimed to compare the gut microbiome found in cirrhotic patients with or without HCC. Materials and methods: From 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition, results were also compared with 25 healthy subjects. Fecal stool samples were sequenced for the V3?V4 region of the microbial 16S rRNA (Illumina MiSeq Platform). Plasma cytokines were quantified including interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Results: We found a differential abundance in family members of Firmicutes with a 3-fold increase of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found to be 5-fold decreased in HCC vs wo-HCC. The ratio bacteriodes/prevotella was increased in HCC. Three operational taxonomic units (OTUs), genus Odoribacter and Butyricimonas were more abundant in HCC, whereas a decreased abundance in Lachnospiraceae family genus Dorea was observed in HCC patients. A Random Forest model trained with differential abundant taxa correctly classified HCC individuals. This pattern was associated with an inflammatory milieu with a putative increased activation of NOD-like receptor pathways. Conclusion: We found a pattern of microbiome linked to inflammation that could be potentially useful as HCC biomarker after follow-up validation studies.
Fil: Piñero, Federico. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina. Universidad Austral; Argentina
Fil: Vazquez, Martín. Heritas; Argentina
Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rohr, Cristian Oscar. Heritas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mendizabal, Manuel. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Fil: Sciara, Mariela Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Heritas; Argentina
Fil: Alonso, Cristina. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Fil: Fay, Fabián. Heritas; Argentina
Fil: Silva, Marcelo. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Materia
INFLAMMATORY PATHWAYS
LIVER CANCER
MICROBIOTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105751
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinomaPiñero, FedericoVazquez, MartínBaré, PatriciaRohr, Cristian OscarMendizabal, ManuelSciara, Mariela InesAlonso, CristinaFay, FabiánSilva, MarceloINFLAMMATORY PATHWAYSLIVER CANCERMICROBIOTAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Introduction and aim: A pro-oncogenic intestinal microbiome was observed in murine models; however, no specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported. We aimed to compare the gut microbiome found in cirrhotic patients with or without HCC. Materials and methods: From 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition, results were also compared with 25 healthy subjects. Fecal stool samples were sequenced for the V3?V4 region of the microbial 16S rRNA (Illumina MiSeq Platform). Plasma cytokines were quantified including interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Results: We found a differential abundance in family members of Firmicutes with a 3-fold increase of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found to be 5-fold decreased in HCC vs wo-HCC. The ratio bacteriodes/prevotella was increased in HCC. Three operational taxonomic units (OTUs), genus Odoribacter and Butyricimonas were more abundant in HCC, whereas a decreased abundance in Lachnospiraceae family genus Dorea was observed in HCC patients. A Random Forest model trained with differential abundant taxa correctly classified HCC individuals. This pattern was associated with an inflammatory milieu with a putative increased activation of NOD-like receptor pathways. Conclusion: We found a pattern of microbiome linked to inflammation that could be potentially useful as HCC biomarker after follow-up validation studies.Fil: Piñero, Federico. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina. Universidad Austral; ArgentinaFil: Vazquez, Martín. Heritas; ArgentinaFil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Rohr, Cristian Oscar. Heritas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mendizabal, Manuel. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; ArgentinaFil: Sciara, Mariela Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Heritas; ArgentinaFil: Alonso, Cristina. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; ArgentinaFil: Fay, Fabián. Heritas; ArgentinaFil: Silva, Marcelo. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; ArgentinaMexican Association of Hepatology2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105751Piñero, Federico; Vazquez, Martín; Baré, Patricia; Rohr, Cristian Oscar; Mendizabal, Manuel; et al.; A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma; Mexican Association of Hepatology; Annals of Hepatology; 18; 3; 5-2019; 480-4871665-2681CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aohep.2018.10.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S166526811930033Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:14Zoai:ri.conicet.gov.ar:11336/105751instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:15.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
title A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
spellingShingle A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
Piñero, Federico
INFLAMMATORY PATHWAYS
LIVER CANCER
MICROBIOTA
title_short A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
title_full A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
title_fullStr A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
title_full_unstemmed A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
title_sort A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma
dc.creator.none.fl_str_mv Piñero, Federico
Vazquez, Martín
Baré, Patricia
Rohr, Cristian Oscar
Mendizabal, Manuel
Sciara, Mariela Ines
Alonso, Cristina
Fay, Fabián
Silva, Marcelo
author Piñero, Federico
author_facet Piñero, Federico
Vazquez, Martín
Baré, Patricia
Rohr, Cristian Oscar
Mendizabal, Manuel
Sciara, Mariela Ines
Alonso, Cristina
Fay, Fabián
Silva, Marcelo
author_role author
author2 Vazquez, Martín
Baré, Patricia
Rohr, Cristian Oscar
Mendizabal, Manuel
Sciara, Mariela Ines
Alonso, Cristina
Fay, Fabián
Silva, Marcelo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFLAMMATORY PATHWAYS
LIVER CANCER
MICROBIOTA
topic INFLAMMATORY PATHWAYS
LIVER CANCER
MICROBIOTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Introduction and aim: A pro-oncogenic intestinal microbiome was observed in murine models; however, no specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported. We aimed to compare the gut microbiome found in cirrhotic patients with or without HCC. Materials and methods: From 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition, results were also compared with 25 healthy subjects. Fecal stool samples were sequenced for the V3?V4 region of the microbial 16S rRNA (Illumina MiSeq Platform). Plasma cytokines were quantified including interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Results: We found a differential abundance in family members of Firmicutes with a 3-fold increase of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found to be 5-fold decreased in HCC vs wo-HCC. The ratio bacteriodes/prevotella was increased in HCC. Three operational taxonomic units (OTUs), genus Odoribacter and Butyricimonas were more abundant in HCC, whereas a decreased abundance in Lachnospiraceae family genus Dorea was observed in HCC patients. A Random Forest model trained with differential abundant taxa correctly classified HCC individuals. This pattern was associated with an inflammatory milieu with a putative increased activation of NOD-like receptor pathways. Conclusion: We found a pattern of microbiome linked to inflammation that could be potentially useful as HCC biomarker after follow-up validation studies.
Fil: Piñero, Federico. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina. Universidad Austral; Argentina
Fil: Vazquez, Martín. Heritas; Argentina
Fil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rohr, Cristian Oscar. Heritas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mendizabal, Manuel. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Fil: Sciara, Mariela Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Heritas; Argentina
Fil: Alonso, Cristina. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
Fil: Fay, Fabián. Heritas; Argentina
Fil: Silva, Marcelo. Universidad Austral; Argentina. Red Latinoamericana de Investigación, Educación y Conciencia del Hígado; Argentina
description Introduction and aim: A pro-oncogenic intestinal microbiome was observed in murine models; however, no specific microbiome in patients with hepatocellular carcinoma (HCC) has been reported. We aimed to compare the gut microbiome found in cirrhotic patients with or without HCC. Materials and methods: From 407 patients with Child Pugh A/B cirrhosis prospectively followed, 25 with HCC (cases) were matched with 25 without HCC (wo-HCC) in a 1:1 ratio according to age, gender, etiology, Child Pugh and severity of portal hypertension. In addition, results were also compared with 25 healthy subjects. Fecal stool samples were sequenced for the V3?V4 region of the microbial 16S rRNA (Illumina MiSeq Platform). Plasma cytokines were quantified including interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). Results: We found a differential abundance in family members of Firmicutes with a 3-fold increase of Erysipelotrichaceae and a 5-fold decrease in family Leuconostocaceae in HCC when compared to wo-HCC controls. Genus Fusobacterium was found to be 5-fold decreased in HCC vs wo-HCC. The ratio bacteriodes/prevotella was increased in HCC. Three operational taxonomic units (OTUs), genus Odoribacter and Butyricimonas were more abundant in HCC, whereas a decreased abundance in Lachnospiraceae family genus Dorea was observed in HCC patients. A Random Forest model trained with differential abundant taxa correctly classified HCC individuals. This pattern was associated with an inflammatory milieu with a putative increased activation of NOD-like receptor pathways. Conclusion: We found a pattern of microbiome linked to inflammation that could be potentially useful as HCC biomarker after follow-up validation studies.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105751
Piñero, Federico; Vazquez, Martín; Baré, Patricia; Rohr, Cristian Oscar; Mendizabal, Manuel; et al.; A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma; Mexican Association of Hepatology; Annals of Hepatology; 18; 3; 5-2019; 480-487
1665-2681
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105751
identifier_str_mv Piñero, Federico; Vazquez, Martín; Baré, Patricia; Rohr, Cristian Oscar; Mendizabal, Manuel; et al.; A different gut microbiome linked to inflammation found in cirrhotic patients with and without hepatocellular carcinoma; Mexican Association of Hepatology; Annals of Hepatology; 18; 3; 5-2019; 480-487
1665-2681
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aohep.2018.10.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S166526811930033X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mexican Association of Hepatology
publisher.none.fl_str_mv Mexican Association of Hepatology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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