Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling

Autores
Gundel, Pedro Emilio; Ueno, Andrea Celeste; Panteix, M.; Iannone, Leopoldo Javier
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some important forage grasses of the genera Festuca and Lolium establish persistent symbiotic associations with vertically-transmitted fungal endophytes (genus Epichloë). In certain cases, the fungus causes livestock diseases due to fungal toxins that accumulate in the plant biomass. Killing the fungus in the seed is a possibility for getting rid of the problem. However, since the symbiosis is mutualistic, the inoculation of elite cultivars with non-toxic but still benefcial endophytes is a current breeding strategy. Additionally, the symbiosis has become a model system to study in ecology and evolution, where the manipulation of the symbiotic status of plants is critical for the experiments. In this study, we confrmed that testing for the endophyte?s presence or absence in the endosperm-side of the seed was a reliable predictor of the symbiotic status of the seedling. We built on this previously proposed concept by (i) estimating the high correspondence between the infection status in one side of the seed (either + or −) and the infection status of the other side, and (ii) demonstrating that cutting the seed in two halves did not afect seed germination, normal seedling growth, nor the endophyte transmission to the seedling. We also showed that cutting the seed reduced seedling size, an impact that increased with endophyte presence provided the fungus was alive. Te strengths and weaknesses of the technique, as well as its potential use in other species, are discussed.
Fil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Ueno, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Panteix, M.. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Iannone, Leopoldo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
Endophyte-plant symbiosis
seed ecophysiology
endophyte fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96149
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96149
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the SeedlingGundel, Pedro EmilioUeno, Andrea CelestePanteix, M.Iannone, Leopoldo JavierEndophyte-plant symbiosisseed ecophysiologyendophyte fungihttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Some important forage grasses of the genera Festuca and Lolium establish persistent symbiotic associations with vertically-transmitted fungal endophytes (genus Epichloë). In certain cases, the fungus causes livestock diseases due to fungal toxins that accumulate in the plant biomass. Killing the fungus in the seed is a possibility for getting rid of the problem. However, since the symbiosis is mutualistic, the inoculation of elite cultivars with non-toxic but still benefcial endophytes is a current breeding strategy. Additionally, the symbiosis has become a model system to study in ecology and evolution, where the manipulation of the symbiotic status of plants is critical for the experiments. In this study, we confrmed that testing for the endophyte?s presence or absence in the endosperm-side of the seed was a reliable predictor of the symbiotic status of the seedling. We built on this previously proposed concept by (i) estimating the high correspondence between the infection status in one side of the seed (either + or −) and the infection status of the other side, and (ii) demonstrating that cutting the seed in two halves did not afect seed germination, normal seedling growth, nor the endophyte transmission to the seedling. We also showed that cutting the seed reduced seedling size, an impact that increased with endophyte presence provided the fungus was alive. Te strengths and weaknesses of the technique, as well as its potential use in other species, are discussed.Fil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Ueno, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Panteix, M.. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Iannone, Leopoldo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaSeed Technology2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96149Gundel, Pedro Emilio; Ueno, Andrea Celeste; Panteix, M. ; Iannone, Leopoldo Javier; Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling; Seed Technology; Seed Technology; 39; 2; 6-2018; 117-1271096-0724CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://stjournal.org/volume-39-no-2-2018/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:56Zoai:ri.conicet.gov.ar:11336/96149instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:56.917CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
title Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
spellingShingle Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
Gundel, Pedro Emilio
Endophyte-plant symbiosis
seed ecophysiology
endophyte fungi
title_short Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
title_full Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
title_fullStr Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
title_full_unstemmed Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
title_sort Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling
dc.creator.none.fl_str_mv Gundel, Pedro Emilio
Ueno, Andrea Celeste
Panteix, M.
Iannone, Leopoldo Javier
author Gundel, Pedro Emilio
author_facet Gundel, Pedro Emilio
Ueno, Andrea Celeste
Panteix, M.
Iannone, Leopoldo Javier
author_role author
author2 Ueno, Andrea Celeste
Panteix, M.
Iannone, Leopoldo Javier
author2_role author
author
author
dc.subject.none.fl_str_mv Endophyte-plant symbiosis
seed ecophysiology
endophyte fungi
topic Endophyte-plant symbiosis
seed ecophysiology
endophyte fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Some important forage grasses of the genera Festuca and Lolium establish persistent symbiotic associations with vertically-transmitted fungal endophytes (genus Epichloë). In certain cases, the fungus causes livestock diseases due to fungal toxins that accumulate in the plant biomass. Killing the fungus in the seed is a possibility for getting rid of the problem. However, since the symbiosis is mutualistic, the inoculation of elite cultivars with non-toxic but still benefcial endophytes is a current breeding strategy. Additionally, the symbiosis has become a model system to study in ecology and evolution, where the manipulation of the symbiotic status of plants is critical for the experiments. In this study, we confrmed that testing for the endophyte?s presence or absence in the endosperm-side of the seed was a reliable predictor of the symbiotic status of the seedling. We built on this previously proposed concept by (i) estimating the high correspondence between the infection status in one side of the seed (either + or −) and the infection status of the other side, and (ii) demonstrating that cutting the seed in two halves did not afect seed germination, normal seedling growth, nor the endophyte transmission to the seedling. We also showed that cutting the seed reduced seedling size, an impact that increased with endophyte presence provided the fungus was alive. Te strengths and weaknesses of the technique, as well as its potential use in other species, are discussed.
Fil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Ueno, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Panteix, M.. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Iannone, Leopoldo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description Some important forage grasses of the genera Festuca and Lolium establish persistent symbiotic associations with vertically-transmitted fungal endophytes (genus Epichloë). In certain cases, the fungus causes livestock diseases due to fungal toxins that accumulate in the plant biomass. Killing the fungus in the seed is a possibility for getting rid of the problem. However, since the symbiosis is mutualistic, the inoculation of elite cultivars with non-toxic but still benefcial endophytes is a current breeding strategy. Additionally, the symbiosis has become a model system to study in ecology and evolution, where the manipulation of the symbiotic status of plants is critical for the experiments. In this study, we confrmed that testing for the endophyte?s presence or absence in the endosperm-side of the seed was a reliable predictor of the symbiotic status of the seedling. We built on this previously proposed concept by (i) estimating the high correspondence between the infection status in one side of the seed (either + or −) and the infection status of the other side, and (ii) demonstrating that cutting the seed in two halves did not afect seed germination, normal seedling growth, nor the endophyte transmission to the seedling. We also showed that cutting the seed reduced seedling size, an impact that increased with endophyte presence provided the fungus was alive. Te strengths and weaknesses of the technique, as well as its potential use in other species, are discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96149
Gundel, Pedro Emilio; Ueno, Andrea Celeste; Panteix, M. ; Iannone, Leopoldo Javier; Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling; Seed Technology; Seed Technology; 39; 2; 6-2018; 117-127
1096-0724
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96149
identifier_str_mv Gundel, Pedro Emilio; Ueno, Andrea Celeste; Panteix, M. ; Iannone, Leopoldo Javier; Presence of Epichloë Fungus in the Endosperm-Side of the Seed Predicts the Symbiotic Status of the Seedling; Seed Technology; Seed Technology; 39; 2; 6-2018; 117-127
1096-0724
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://stjournal.org/volume-39-no-2-2018/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Seed Technology
publisher.none.fl_str_mv Seed Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269003383308288
score 13.13397

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