Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia

Autores
Rodriguez, Yamila Eliana; Laitano, Marìa Victoria; Pereira, Nair de Los Angeles; López Zavala, Alonso A.; Haran, Nora Selma; Fernandez Gimenez, Analia Veronica
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shrimp processing waste holds digestive proteases with a great potential to be used as feed supplement for Nile tilapia Oreochromis niloticus. In the present work, we characterize both sodium alginate (A) and sodium alginate-bentonite (AB) microcapsules to entrap enzymes obtained from Pleoticus muelleri processing waste. Also, we evaluate these encapsulation methods as a strategy to improve intestinal delivery of exogenous enzymes in O. niloticus in order to enhance their digestion process. The effects of different storage methods, in vitro simulation of gastric pH conditions and exposition to 40 °C were studied. In order to evaluate if microencapsulated shrimp enzymes are active when they reach fish gut, animals were exposed to three treatments: (1) fast, (2) diet and, (3) AB capsules + diet. Alginate capsules were more affected by different storage methods than alginate-bentonite ones. SEM images showed a correlation between decreased enzyme activity and capsule microstructure changes. The best method to store the AB beads is at −20 °C. After incubating for 7 h at 40 °C, we observed a notorious reduction in the enzyme activities of both microcapsules. On the other hand, at pH 3 both microcapsules prevented enzyme irreversible denaturalization and kept 100% of their activity. The overall results indicate that AB capsules are better vehicles to deliver shrimp enzymes in Nile tilapia. In the bioassay, we observed that when fish were fed with treatment 3, the alkaline protease activity in their intestines was 27% higher than that of the diet fish group. Thus, encapsulated shrimp enzymes have a great potential to be used as a feed supplement in fish nutrition. Further trials involving grow bioassays are needed to verify if this shrimp enzyme contained in AB capsules improve fish digestion, feed conversion, body weight and survival rate.
Fil: Rodriguez, Yamila Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Laitano, Marìa Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: López Zavala, Alonso A.. Universidad de Sonora; México
Fil: Haran, Nora Selma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
EXOGENOUS ENZYMES DELIVERY
MICROCAPSULES
OREOCHROMIS NILOTICUS
SHRIMP PROCESSING WASTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87594
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/87594
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapiaRodriguez, Yamila ElianaLaitano, Marìa VictoriaPereira, Nair de Los AngelesLópez Zavala, Alonso A.Haran, Nora SelmaFernandez Gimenez, Analia VeronicaEXOGENOUS ENZYMES DELIVERYMICROCAPSULESOREOCHROMIS NILOTICUSSHRIMP PROCESSING WASTEhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Shrimp processing waste holds digestive proteases with a great potential to be used as feed supplement for Nile tilapia Oreochromis niloticus. In the present work, we characterize both sodium alginate (A) and sodium alginate-bentonite (AB) microcapsules to entrap enzymes obtained from Pleoticus muelleri processing waste. Also, we evaluate these encapsulation methods as a strategy to improve intestinal delivery of exogenous enzymes in O. niloticus in order to enhance their digestion process. The effects of different storage methods, in vitro simulation of gastric pH conditions and exposition to 40 °C were studied. In order to evaluate if microencapsulated shrimp enzymes are active when they reach fish gut, animals were exposed to three treatments: (1) fast, (2) diet and, (3) AB capsules + diet. Alginate capsules were more affected by different storage methods than alginate-bentonite ones. SEM images showed a correlation between decreased enzyme activity and capsule microstructure changes. The best method to store the AB beads is at −20 °C. After incubating for 7 h at 40 °C, we observed a notorious reduction in the enzyme activities of both microcapsules. On the other hand, at pH 3 both microcapsules prevented enzyme irreversible denaturalization and kept 100% of their activity. The overall results indicate that AB capsules are better vehicles to deliver shrimp enzymes in Nile tilapia. In the bioassay, we observed that when fish were fed with treatment 3, the alkaline protease activity in their intestines was 27% higher than that of the diet fish group. Thus, encapsulated shrimp enzymes have a great potential to be used as a feed supplement in fish nutrition. Further trials involving grow bioassays are needed to verify if this shrimp enzyme contained in AB capsules improve fish digestion, feed conversion, body weight and survival rate.Fil: Rodriguez, Yamila Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Laitano, Marìa Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: López Zavala, Alonso A.. Universidad de Sonora; MéxicoFil: Haran, Nora Selma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaElsevier Science2018-01-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87594Rodriguez, Yamila Eliana; Laitano, Marìa Victoria; Pereira, Nair de Los Angeles; López Zavala, Alonso A.; Haran, Nora Selma; et al.; Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia; Elsevier Science; Aquaculture; 490; 20-1-2018; 35-430044-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.02.022info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0044848617323232info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:36Zoai:ri.conicet.gov.ar:11336/87594instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:36.979CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
title Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
spellingShingle Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
Rodriguez, Yamila Eliana
EXOGENOUS ENZYMES DELIVERY
MICROCAPSULES
OREOCHROMIS NILOTICUS
SHRIMP PROCESSING WASTE
title_short Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
title_full Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
title_fullStr Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
title_full_unstemmed Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
title_sort Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia
dc.creator.none.fl_str_mv Rodriguez, Yamila Eliana
Laitano, Marìa Victoria
Pereira, Nair de Los Angeles
López Zavala, Alonso A.
Haran, Nora Selma
Fernandez Gimenez, Analia Veronica
author Rodriguez, Yamila Eliana
author_facet Rodriguez, Yamila Eliana
Laitano, Marìa Victoria
Pereira, Nair de Los Angeles
López Zavala, Alonso A.
Haran, Nora Selma
Fernandez Gimenez, Analia Veronica
author_role author
author2 Laitano, Marìa Victoria
Pereira, Nair de Los Angeles
López Zavala, Alonso A.
Haran, Nora Selma
Fernandez Gimenez, Analia Veronica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EXOGENOUS ENZYMES DELIVERY
MICROCAPSULES
OREOCHROMIS NILOTICUS
SHRIMP PROCESSING WASTE
topic EXOGENOUS ENZYMES DELIVERY
MICROCAPSULES
OREOCHROMIS NILOTICUS
SHRIMP PROCESSING WASTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Shrimp processing waste holds digestive proteases with a great potential to be used as feed supplement for Nile tilapia Oreochromis niloticus. In the present work, we characterize both sodium alginate (A) and sodium alginate-bentonite (AB) microcapsules to entrap enzymes obtained from Pleoticus muelleri processing waste. Also, we evaluate these encapsulation methods as a strategy to improve intestinal delivery of exogenous enzymes in O. niloticus in order to enhance their digestion process. The effects of different storage methods, in vitro simulation of gastric pH conditions and exposition to 40 °C were studied. In order to evaluate if microencapsulated shrimp enzymes are active when they reach fish gut, animals were exposed to three treatments: (1) fast, (2) diet and, (3) AB capsules + diet. Alginate capsules were more affected by different storage methods than alginate-bentonite ones. SEM images showed a correlation between decreased enzyme activity and capsule microstructure changes. The best method to store the AB beads is at −20 °C. After incubating for 7 h at 40 °C, we observed a notorious reduction in the enzyme activities of both microcapsules. On the other hand, at pH 3 both microcapsules prevented enzyme irreversible denaturalization and kept 100% of their activity. The overall results indicate that AB capsules are better vehicles to deliver shrimp enzymes in Nile tilapia. In the bioassay, we observed that when fish were fed with treatment 3, the alkaline protease activity in their intestines was 27% higher than that of the diet fish group. Thus, encapsulated shrimp enzymes have a great potential to be used as a feed supplement in fish nutrition. Further trials involving grow bioassays are needed to verify if this shrimp enzyme contained in AB capsules improve fish digestion, feed conversion, body weight and survival rate.
Fil: Rodriguez, Yamila Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Laitano, Marìa Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Pereira, Nair de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: López Zavala, Alonso A.. Universidad de Sonora; México
Fil: Haran, Nora Selma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Shrimp processing waste holds digestive proteases with a great potential to be used as feed supplement for Nile tilapia Oreochromis niloticus. In the present work, we characterize both sodium alginate (A) and sodium alginate-bentonite (AB) microcapsules to entrap enzymes obtained from Pleoticus muelleri processing waste. Also, we evaluate these encapsulation methods as a strategy to improve intestinal delivery of exogenous enzymes in O. niloticus in order to enhance their digestion process. The effects of different storage methods, in vitro simulation of gastric pH conditions and exposition to 40 °C were studied. In order to evaluate if microencapsulated shrimp enzymes are active when they reach fish gut, animals were exposed to three treatments: (1) fast, (2) diet and, (3) AB capsules + diet. Alginate capsules were more affected by different storage methods than alginate-bentonite ones. SEM images showed a correlation between decreased enzyme activity and capsule microstructure changes. The best method to store the AB beads is at −20 °C. After incubating for 7 h at 40 °C, we observed a notorious reduction in the enzyme activities of both microcapsules. On the other hand, at pH 3 both microcapsules prevented enzyme irreversible denaturalization and kept 100% of their activity. The overall results indicate that AB capsules are better vehicles to deliver shrimp enzymes in Nile tilapia. In the bioassay, we observed that when fish were fed with treatment 3, the alkaline protease activity in their intestines was 27% higher than that of the diet fish group. Thus, encapsulated shrimp enzymes have a great potential to be used as a feed supplement in fish nutrition. Further trials involving grow bioassays are needed to verify if this shrimp enzyme contained in AB capsules improve fish digestion, feed conversion, body weight and survival rate.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87594
Rodriguez, Yamila Eliana; Laitano, Marìa Victoria; Pereira, Nair de Los Angeles; López Zavala, Alonso A.; Haran, Nora Selma; et al.; Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia; Elsevier Science; Aquaculture; 490; 20-1-2018; 35-43
0044-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87594
identifier_str_mv Rodriguez, Yamila Eliana; Laitano, Marìa Victoria; Pereira, Nair de Los Angeles; López Zavala, Alonso A.; Haran, Nora Selma; et al.; Exogenous enzymes in aquaculture: Alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia; Elsevier Science; Aquaculture; 490; 20-1-2018; 35-43
0044-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2018.02.022
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0044848617323232
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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