Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids

Autores
Zampini, Iris Catiana; Francisco, M.; Nuño, Gabriela; Ordóñez, Roxana Mabel; Isla, Maria Ines; Piloto Ferrer, J.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Zuccagnia punctata is a shrub from Fabaceae family which commonly grows distributed in arid and semiarid areas in western Argentina. This plant is used in ethnomedicine as antimicrobial and anti-inflammatory drug. Some biological properties were reported for Z. punctata extracts (antioxidant, anti-inflamatory, antigenotoxic, antimicrobial). The aim of the present study was to determine the in vitro cytotoxic effects of an ethanolic extract from Z. punctata aerial part and four of their flavonoids: 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF), 2?,4?-dihydroxy-3?-methoxychalcone (DHMC) and 2?,4?-dihydroxychalcone (DHC) using mammalian cell cultures. The cytotoxicity of the natural products was tested using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) viability assay on MCF-7 human breast adenocarcinoma and CT26 mouse colon adenocarcinoma cells. The assays were performed at 24, 48 and 72 h. The results demonstrated that the Z. punctata ethanolic extract and their flavonoids induced cytotoxic effects on the two cell lines evaluated. The activity on cell lines after 72 h of exposing was in the following order: DHC >DHCM>DHF>Zp with IC50 values between 3.125 - 50 µg/ml and HF at the highest concentration (50 µg/ml) evaluated affected only 20 % the cell viability in all cases. The active samples reduced CT26 cell viability in a concentration-dependent manner and the effect increased with the incubation time. However, the activity of the active flavonoids was stronger on MCF7 and started from 72 h of treatment. These results suggest that the studied natural product could be applied as natural anticancer drug.
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Francisco, M.. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Nuño, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Piloto Ferrer, J.. Centro de Investigación y Desarrollo de Medicamentos; Cuba
14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicaciones
Puerto Varas
Chile
Universidad Nacional de Talca
Materia
Zuccagnia punctata
Flavonoides
Citotoxicidad
Ensayo MTT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238205
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoidsZampini, Iris CatianaFrancisco, M.Nuño, GabrielaOrdóñez, Roxana MabelIsla, Maria InesPiloto Ferrer, J.Zuccagnia punctataFlavonoidesCitotoxicidadEnsayo MTThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Zuccagnia punctata is a shrub from Fabaceae family which commonly grows distributed in arid and semiarid areas in western Argentina. This plant is used in ethnomedicine as antimicrobial and anti-inflammatory drug. Some biological properties were reported for Z. punctata extracts (antioxidant, anti-inflamatory, antigenotoxic, antimicrobial). The aim of the present study was to determine the in vitro cytotoxic effects of an ethanolic extract from Z. punctata aerial part and four of their flavonoids: 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF), 2?,4?-dihydroxy-3?-methoxychalcone (DHMC) and 2?,4?-dihydroxychalcone (DHC) using mammalian cell cultures. The cytotoxicity of the natural products was tested using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) viability assay on MCF-7 human breast adenocarcinoma and CT26 mouse colon adenocarcinoma cells. The assays were performed at 24, 48 and 72 h. The results demonstrated that the Z. punctata ethanolic extract and their flavonoids induced cytotoxic effects on the two cell lines evaluated. The activity on cell lines after 72 h of exposing was in the following order: DHC >DHCM>DHF>Zp with IC50 values between 3.125 - 50 µg/ml and HF at the highest concentration (50 µg/ml) evaluated affected only 20 % the cell viability in all cases. The active samples reduced CT26 cell viability in a concentration-dependent manner and the effect increased with the incubation time. However, the activity of the active flavonoids was stronger on MCF7 and started from 72 h of treatment. These results suggest that the studied natural product could be applied as natural anticancer drug.Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; ArgentinaFil: Francisco, M.. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: Nuño, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; ArgentinaFil: Piloto Ferrer, J.. Centro de Investigación y Desarrollo de Medicamentos; Cuba14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicacionesPuerto VarasChileUniversidad Nacional de TalcaUniversidad de Talca2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238205Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids; 14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicaciones; Puerto Varas; Chile; 2014; 166-166CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bib.irb.hr/datoteka/742320.Book_of_Abstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:22Zoai:ri.conicet.gov.ar:11336/238205instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:22.656CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
title Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
spellingShingle Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
Zampini, Iris Catiana
Zuccagnia punctata
Flavonoides
Citotoxicidad
Ensayo MTT
title_short Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
title_full Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
title_fullStr Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
title_full_unstemmed Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
title_sort Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids
dc.creator.none.fl_str_mv Zampini, Iris Catiana
Francisco, M.
Nuño, Gabriela
Ordóñez, Roxana Mabel
Isla, Maria Ines
Piloto Ferrer, J.
author Zampini, Iris Catiana
author_facet Zampini, Iris Catiana
Francisco, M.
Nuño, Gabriela
Ordóñez, Roxana Mabel
Isla, Maria Ines
Piloto Ferrer, J.
author_role author
author2 Francisco, M.
Nuño, Gabriela
Ordóñez, Roxana Mabel
Isla, Maria Ines
Piloto Ferrer, J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Zuccagnia punctata
Flavonoides
Citotoxicidad
Ensayo MTT
topic Zuccagnia punctata
Flavonoides
Citotoxicidad
Ensayo MTT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Zuccagnia punctata is a shrub from Fabaceae family which commonly grows distributed in arid and semiarid areas in western Argentina. This plant is used in ethnomedicine as antimicrobial and anti-inflammatory drug. Some biological properties were reported for Z. punctata extracts (antioxidant, anti-inflamatory, antigenotoxic, antimicrobial). The aim of the present study was to determine the in vitro cytotoxic effects of an ethanolic extract from Z. punctata aerial part and four of their flavonoids: 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF), 2?,4?-dihydroxy-3?-methoxychalcone (DHMC) and 2?,4?-dihydroxychalcone (DHC) using mammalian cell cultures. The cytotoxicity of the natural products was tested using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) viability assay on MCF-7 human breast adenocarcinoma and CT26 mouse colon adenocarcinoma cells. The assays were performed at 24, 48 and 72 h. The results demonstrated that the Z. punctata ethanolic extract and their flavonoids induced cytotoxic effects on the two cell lines evaluated. The activity on cell lines after 72 h of exposing was in the following order: DHC >DHCM>DHF>Zp with IC50 values between 3.125 - 50 µg/ml and HF at the highest concentration (50 µg/ml) evaluated affected only 20 % the cell viability in all cases. The active samples reduced CT26 cell viability in a concentration-dependent manner and the effect increased with the incubation time. However, the activity of the active flavonoids was stronger on MCF7 and started from 72 h of treatment. These results suggest that the studied natural product could be applied as natural anticancer drug.
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Francisco, M.. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Nuño, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; Argentina
Fil: Piloto Ferrer, J.. Centro de Investigación y Desarrollo de Medicamentos; Cuba
14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicaciones
Puerto Varas
Chile
Universidad Nacional de Talca
description Zuccagnia punctata is a shrub from Fabaceae family which commonly grows distributed in arid and semiarid areas in western Argentina. This plant is used in ethnomedicine as antimicrobial and anti-inflammatory drug. Some biological properties were reported for Z. punctata extracts (antioxidant, anti-inflamatory, antigenotoxic, antimicrobial). The aim of the present study was to determine the in vitro cytotoxic effects of an ethanolic extract from Z. punctata aerial part and four of their flavonoids: 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF), 2?,4?-dihydroxy-3?-methoxychalcone (DHMC) and 2?,4?-dihydroxychalcone (DHC) using mammalian cell cultures. The cytotoxicity of the natural products was tested using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) viability assay on MCF-7 human breast adenocarcinoma and CT26 mouse colon adenocarcinoma cells. The assays were performed at 24, 48 and 72 h. The results demonstrated that the Z. punctata ethanolic extract and their flavonoids induced cytotoxic effects on the two cell lines evaluated. The activity on cell lines after 72 h of exposing was in the following order: DHC >DHCM>DHF>Zp with IC50 values between 3.125 - 50 µg/ml and HF at the highest concentration (50 µg/ml) evaluated affected only 20 % the cell viability in all cases. The active samples reduced CT26 cell viability in a concentration-dependent manner and the effect increased with the incubation time. However, the activity of the active flavonoids was stronger on MCF7 and started from 72 h of treatment. These results suggest that the studied natural product could be applied as natural anticancer drug.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238205
Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids; 14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicaciones; Puerto Varas; Chile; 2014; 166-166
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238205
identifier_str_mv Cytotoxic effect of Zuccagnia punctata ethanolic extract and their flavonoids; 14th International Congress of Ethnopharmacology; VII Simposio de Química de Productos Naturales y sus aplicaciones; Puerto Varas; Chile; 2014; 166-166
CONICET Digital
CONICET
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language eng
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad de Talca
publisher.none.fl_str_mv Universidad de Talca
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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