Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors

Autores
Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; Calabuig, E.; Iranzo, A.; Barcan, L. A.; Anders, M.; Schijman, Alejandro Gabriel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.
Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Nagel, C.. Universidad Favaloro; Argentina
Fil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Barcan, L. A.. Hospital Italiano de Buenos Aires; Argentina
Fil: Anders, M.. Hospital Alemán; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Materia
Chagas
Transplante
Trypanosoma Cruzi
Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3976
Acceder
id CONICETDig_11fd84c1cea4a3ef2805e17862bd6bbc
oai_identifier_str oai:ri.conicet.gov.ar:11336/3976
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donorsCura, Carolina InésLattes, R.Nagel, C.Gimenez, M. J.Blanes, M.Calabuig, E.Iranzo, A.Barcan, L. A.Anders, M.Schijman, Alejandro GabrielChagasTransplanteTrypanosoma CruziPcrhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Nagel, C.. Universidad Favaloro; ArgentinaFil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; EspañaFil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; EspañaFil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; EspañaFil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; EspañaFil: Barcan, L. A.. Hospital Italiano de Buenos Aires; ArgentinaFil: Anders, M.. Hospital Alemán; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaWiley2013-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3976Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-32611600-6135enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajt.12487/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/ajt.12487info:eu-repo/semantics/altIdentifier/issn/1600-6135info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:48Zoai:ri.conicet.gov.ar:11336/3976instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:49.118CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
title Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
spellingShingle Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
Cura, Carolina Inés
Chagas
Transplante
Trypanosoma Cruzi
Pcr
title_short Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
title_full Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
title_fullStr Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
title_full_unstemmed Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
title_sort Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
dc.creator.none.fl_str_mv Cura, Carolina Inés
Lattes, R.
Nagel, C.
Gimenez, M. J.
Blanes, M.
Calabuig, E.
Iranzo, A.
Barcan, L. A.
Anders, M.
Schijman, Alejandro Gabriel
author Cura, Carolina Inés
author_facet Cura, Carolina Inés
Lattes, R.
Nagel, C.
Gimenez, M. J.
Blanes, M.
Calabuig, E.
Iranzo, A.
Barcan, L. A.
Anders, M.
Schijman, Alejandro Gabriel
author_role author
author2 Lattes, R.
Nagel, C.
Gimenez, M. J.
Blanes, M.
Calabuig, E.
Iranzo, A.
Barcan, L. A.
Anders, M.
Schijman, Alejandro Gabriel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas
Transplante
Trypanosoma Cruzi
Pcr
topic Chagas
Transplante
Trypanosoma Cruzi
Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.
Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Nagel, C.. Universidad Favaloro; Argentina
Fil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Barcan, L. A.. Hospital Italiano de Buenos Aires; Argentina
Fil: Anders, M.. Hospital Alemán; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
description Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3976
Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-3261
1600-6135
url http://hdl.handle.net/11336/3976
identifier_str_mv Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-3261
1600-6135
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajt.12487/abstract
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/ajt.12487
info:eu-repo/semantics/altIdentifier/issn/1600-6135
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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