Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors
- Autores
- Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; Calabuig, E.; Iranzo, A.; Barcan, L. A.; Anders, M.; Schijman, Alejandro Gabriel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.
Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
Fil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Nagel, C.. Universidad Favaloro; Argentina
Fil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España
Fil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España
Fil: Barcan, L. A.. Hospital Italiano de Buenos Aires; Argentina
Fil: Anders, M.. Hospital Alemán; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina - Materia
-
Chagas
Transplante
Trypanosoma Cruzi
Pcr - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/3976
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donorsCura, Carolina InésLattes, R.Nagel, C.Gimenez, M. J.Blanes, M.Calabuig, E.Iranzo, A.Barcan, L. A.Anders, M.Schijman, Alejandro GabrielChagasTransplanteTrypanosoma CruziPcrhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment.Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Nagel, C.. Universidad Favaloro; ArgentinaFil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; EspañaFil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; EspañaFil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; EspañaFil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; EspañaFil: Barcan, L. A.. Hospital Italiano de Buenos Aires; ArgentinaFil: Anders, M.. Hospital Alemán; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaWiley2013-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3976Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-32611600-6135enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajt.12487/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/ajt.12487info:eu-repo/semantics/altIdentifier/issn/1600-6135info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:40Zoai:ri.conicet.gov.ar:11336/3976instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:41.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
title |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
spellingShingle |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors Cura, Carolina Inés Chagas Transplante Trypanosoma Cruzi Pcr |
title_short |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
title_full |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
title_fullStr |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
title_full_unstemmed |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
title_sort |
Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors |
dc.creator.none.fl_str_mv |
Cura, Carolina Inés Lattes, R. Nagel, C. Gimenez, M. J. Blanes, M. Calabuig, E. Iranzo, A. Barcan, L. A. Anders, M. Schijman, Alejandro Gabriel |
author |
Cura, Carolina Inés |
author_facet |
Cura, Carolina Inés Lattes, R. Nagel, C. Gimenez, M. J. Blanes, M. Calabuig, E. Iranzo, A. Barcan, L. A. Anders, M. Schijman, Alejandro Gabriel |
author_role |
author |
author2 |
Lattes, R. Nagel, C. Gimenez, M. J. Blanes, M. Calabuig, E. Iranzo, A. Barcan, L. A. Anders, M. Schijman, Alejandro Gabriel |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Chagas Transplante Trypanosoma Cruzi Pcr |
topic |
Chagas Transplante Trypanosoma Cruzi Pcr |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment. Fil: Cura, Carolina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina Fil: Lattes, R.. Instituto de Nefrologia de Buenos Aires; Argentina Fil: Nagel, C.. Universidad Favaloro; Argentina Fil: Gimenez, M. J.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España Fil: Blanes, M.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España Fil: Calabuig, E.. Hospital Universitario y Politécnico LA FE. Area Médica. Unidad de Enfermedades Infecciosas; España Fil: Iranzo, A.. Hospital Universitario y Politécnico LA FE. Servicio de Microbiología; España Fil: Barcan, L. A.. Hospital Italiano de Buenos Aires; Argentina Fil: Anders, M.. Hospital Alemán; Argentina Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina |
description |
Organ transplantation (TX) is a novel transmission modality of Chagas disease. The results of molecular diagnosis and characterization of Trypanosoma cruzi acute infection in naïve TX recipients transplanted with organs from infected deceased donors are reported. Peripheral blood and cerebrospinal fluid samples from the TX recipients of organs from infected donors were prospectively and sequentially studied for detection of T. cruzi by means of kinetoplastid DNA polymerase chain reaction (kDNA-PCR). In positive blood samples, a PCR algorithm for identification of T. cruzi Discrete Typing Units (DTUs) and quantitative real-time PCR (qPCR) to quantify parasitic loads were performed. Minicircle signatures of T. cruzi infecting populations were also analyzed using restriction fragment length polymorphism (RFLP)-PCR. Eight seronegative TX recipients from four infected donors were studied. In five, the infection was detected at 68.4 days post-TX (36–98 days). In one case, it was transmitted to two of three TX recipients. The comparison of the minicircle signatures revealed nearly identical RFLP-PCR profiles, confirming a common source of infection. The five cases were infected by DTU TcV. This report reveals the relevance of systematic monitoring of TX recipients using PCR strategies in order to provide an early diagnosis allowing timely anti-trypanosomal treatment. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-30 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/3976 Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-3261 1600-6135 |
url |
http://hdl.handle.net/11336/3976 |
identifier_str_mv |
Cura, Carolina Inés; Lattes, R.; Nagel, C.; Gimenez, M. J.; Blanes, M.; et al.; Early molecular diagnosis of acute Chagas disease after transplantation with organs from Trypanosoma cruzi-infected donors; Wiley; American Journal of Transplantation; 13; 12; 30-10-2013; 3253-3261 1600-6135 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajt.12487/abstract info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/ajt.12487 info:eu-repo/semantics/altIdentifier/issn/1600-6135 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614397366894592 |
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13.070432 |