More Cercospora species infect soybeans across the Americas than meets the eye
- Autores
- Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; Grijalba, Pablo Enrique; Gottlieb, Alexandra Marina; Bluhm, Burton H.; De Oliveira, Luiz Orlando
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.
Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; Brasil
Fil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Borges, Leandro Luiz. Universidade Federal de Viçosa; Brasil
Fil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; Brasil
Fil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Bluhm, Burton H.. University of Arkansas; Estados Unidos
Fil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; Brasil - Materia
-
SOYBEAN
PHYLOGENETIC
ANALYSIS
PHYLOGEOGRAPHY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/60607
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More Cercospora species infect soybeans across the Americas than meets the eyeSoares, Ana Paula GomesGuillin, Eduardo A.Borges, Leandro LuizDa Silva, Amanda C. T.De Almeida, Álvaro M. R.Grijalba, Pablo EnriqueGottlieb, Alexandra MarinaBluhm, Burton H.De Oliveira, Luiz OrlandoSOYBEANPHYLOGENETICANALYSISPHYLOGEOGRAPHYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; BrasilFil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Borges, Leandro Luiz. Universidade Federal de Viçosa; BrasilFil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; BrasilFil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Bluhm, Burton H.. University of Arkansas; Estados UnidosFil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; BrasilPublic Library of Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60607Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-201932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133495info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133495info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:41Zoai:ri.conicet.gov.ar:11336/60607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:42.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
More Cercospora species infect soybeans across the Americas than meets the eye |
title |
More Cercospora species infect soybeans across the Americas than meets the eye |
spellingShingle |
More Cercospora species infect soybeans across the Americas than meets the eye Soares, Ana Paula Gomes SOYBEAN PHYLOGENETIC ANALYSIS PHYLOGEOGRAPHY |
title_short |
More Cercospora species infect soybeans across the Americas than meets the eye |
title_full |
More Cercospora species infect soybeans across the Americas than meets the eye |
title_fullStr |
More Cercospora species infect soybeans across the Americas than meets the eye |
title_full_unstemmed |
More Cercospora species infect soybeans across the Americas than meets the eye |
title_sort |
More Cercospora species infect soybeans across the Americas than meets the eye |
dc.creator.none.fl_str_mv |
Soares, Ana Paula Gomes Guillin, Eduardo A. Borges, Leandro Luiz Da Silva, Amanda C. T. De Almeida, Álvaro M. R. Grijalba, Pablo Enrique Gottlieb, Alexandra Marina Bluhm, Burton H. De Oliveira, Luiz Orlando |
author |
Soares, Ana Paula Gomes |
author_facet |
Soares, Ana Paula Gomes Guillin, Eduardo A. Borges, Leandro Luiz Da Silva, Amanda C. T. De Almeida, Álvaro M. R. Grijalba, Pablo Enrique Gottlieb, Alexandra Marina Bluhm, Burton H. De Oliveira, Luiz Orlando |
author_role |
author |
author2 |
Guillin, Eduardo A. Borges, Leandro Luiz Da Silva, Amanda C. T. De Almeida, Álvaro M. R. Grijalba, Pablo Enrique Gottlieb, Alexandra Marina Bluhm, Burton H. De Oliveira, Luiz Orlando |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
SOYBEAN PHYLOGENETIC ANALYSIS PHYLOGEOGRAPHY |
topic |
SOYBEAN PHYLOGENETIC ANALYSIS PHYLOGEOGRAPHY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; Brasil Fil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina Fil: Borges, Leandro Luiz. Universidade Federal de Viçosa; Brasil Fil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; Brasil Fil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Bluhm, Burton H.. University of Arkansas; Estados Unidos Fil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; Brasil |
description |
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/60607 Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/60607 |
identifier_str_mv |
Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133495 info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133495 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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