More Cercospora species infect soybeans across the Americas than meets the eye

Autores
Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; Grijalba, Pablo Enrique; Gottlieb, Alexandra Marina; Bluhm, Burton H.; De Oliveira, Luiz Orlando
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.
Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; Brasil
Fil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Borges, Leandro Luiz. Universidade Federal de Viçosa; Brasil
Fil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; Brasil
Fil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Bluhm, Burton H.. University of Arkansas; Estados Unidos
Fil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; Brasil
Materia
SOYBEAN
PHYLOGENETIC
ANALYSIS
PHYLOGEOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60607

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network_name_str CONICET Digital (CONICET)
spelling More Cercospora species infect soybeans across the Americas than meets the eyeSoares, Ana Paula GomesGuillin, Eduardo A.Borges, Leandro LuizDa Silva, Amanda C. T.De Almeida, Álvaro M. R.Grijalba, Pablo EnriqueGottlieb, Alexandra MarinaBluhm, Burton H.De Oliveira, Luiz OrlandoSOYBEANPHYLOGENETICANALYSISPHYLOGEOGRAPHYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; BrasilFil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Borges, Leandro Luiz. Universidade Federal de Viçosa; BrasilFil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; BrasilFil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Bluhm, Burton H.. University of Arkansas; Estados UnidosFil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; BrasilPublic Library of Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60607Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-201932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0133495info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133495info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:41Zoai:ri.conicet.gov.ar:11336/60607instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:42.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv More Cercospora species infect soybeans across the Americas than meets the eye
title More Cercospora species infect soybeans across the Americas than meets the eye
spellingShingle More Cercospora species infect soybeans across the Americas than meets the eye
Soares, Ana Paula Gomes
SOYBEAN
PHYLOGENETIC
ANALYSIS
PHYLOGEOGRAPHY
title_short More Cercospora species infect soybeans across the Americas than meets the eye
title_full More Cercospora species infect soybeans across the Americas than meets the eye
title_fullStr More Cercospora species infect soybeans across the Americas than meets the eye
title_full_unstemmed More Cercospora species infect soybeans across the Americas than meets the eye
title_sort More Cercospora species infect soybeans across the Americas than meets the eye
dc.creator.none.fl_str_mv Soares, Ana Paula Gomes
Guillin, Eduardo A.
Borges, Leandro Luiz
Da Silva, Amanda C. T.
De Almeida, Álvaro M. R.
Grijalba, Pablo Enrique
Gottlieb, Alexandra Marina
Bluhm, Burton H.
De Oliveira, Luiz Orlando
author Soares, Ana Paula Gomes
author_facet Soares, Ana Paula Gomes
Guillin, Eduardo A.
Borges, Leandro Luiz
Da Silva, Amanda C. T.
De Almeida, Álvaro M. R.
Grijalba, Pablo Enrique
Gottlieb, Alexandra Marina
Bluhm, Burton H.
De Oliveira, Luiz Orlando
author_role author
author2 Guillin, Eduardo A.
Borges, Leandro Luiz
Da Silva, Amanda C. T.
De Almeida, Álvaro M. R.
Grijalba, Pablo Enrique
Gottlieb, Alexandra Marina
Bluhm, Burton H.
De Oliveira, Luiz Orlando
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SOYBEAN
PHYLOGENETIC
ANALYSIS
PHYLOGEOGRAPHY
topic SOYBEAN
PHYLOGENETIC
ANALYSIS
PHYLOGEOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.
Fil: Soares, Ana Paula Gomes. Universidade Federal de Viçosa; Brasil
Fil: Guillin, Eduardo A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Borges, Leandro Luiz. Universidade Federal de Viçosa; Brasil
Fil: Da Silva, Amanda C. T.. Universidade Federal de Viçosa; Brasil
Fil: De Almeida, Álvaro M. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Grijalba, Pablo Enrique. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Bluhm, Burton H.. University of Arkansas; Estados Unidos
Fil: De Oliveira, Luiz Orlando. Universidade Federal de Viçosa; Brasil
description Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
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info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60607
Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60607
identifier_str_mv Soares, Ana Paula Gomes; Guillin, Eduardo A.; Borges, Leandro Luiz; Da Silva, Amanda C. T.; De Almeida, Álvaro M. R.; et al.; More Cercospora species infect soybeans across the Americas than meets the eye; Public Library of Science; Plos One; 10; 8; 8-2015; 1-20
1932-6203
CONICET Digital
CONICET
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language eng
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