Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis

Autores
Serrano, María de Los Angeles; Luque, Melchor Emilio; Sanchez, Sara Serafina del V.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake.
Fil: Serrano, María de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Luque, Melchor Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Materia
Camp
Ip3/Ca2+
Vitellogenesis
Xenopus Laevis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21989
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesisSerrano, María de Los AngelesLuque, Melchor EmilioSanchez, Sara Serafina del V.CampIp3/Ca2+VitellogenesisXenopus Laevishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake.Fil: Serrano, María de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Luque, Melchor Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaFil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; ArgentinaCambridge University Press2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21989Serrano, María de Los Angeles; Luque, Melchor Emilio; Sanchez, Sara Serafina del V.; Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis; Cambridge University Press; Zygote; 23; 1; 7-2013; 99-1100967-1994CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199413000324info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/xepac-protein-and-ip3ca2-pathway-implication-during-xenopus-laevis-vitellogenesis/04B119C289520A30E49BC46E8F534D0Einfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:18Zoai:ri.conicet.gov.ar:11336/21989instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:18.909CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
title Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
spellingShingle Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
Serrano, María de Los Angeles
Camp
Ip3/Ca2+
Vitellogenesis
Xenopus Laevis
title_short Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
title_full Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
title_fullStr Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
title_full_unstemmed Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
title_sort Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis
dc.creator.none.fl_str_mv Serrano, María de Los Angeles
Luque, Melchor Emilio
Sanchez, Sara Serafina del V.
author Serrano, María de Los Angeles
author_facet Serrano, María de Los Angeles
Luque, Melchor Emilio
Sanchez, Sara Serafina del V.
author_role author
author2 Luque, Melchor Emilio
Sanchez, Sara Serafina del V.
author2_role author
author
dc.subject.none.fl_str_mv Camp
Ip3/Ca2+
Vitellogenesis
Xenopus Laevis
topic Camp
Ip3/Ca2+
Vitellogenesis
Xenopus Laevis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake.
Fil: Serrano, María de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Luque, Melchor Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
Fil: Sanchez, Sara Serafina del V.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
description The objective of this study was to elucidate the signalling pathways initiated by cAMP once inside the Xenopus laevis oocyte, where it triggers and maintains vitellogenin endocytic uptake. Our results showed the presence of Xepac transcripts at all stages of oogenesis and we demonstrated that a cAMP analogue that exclusively activates Xepac, 8-CPT, was able to rescue the endocytic activity in oocytes with uncoupled gap junctions. Inhibition experiments for the IP3/Ca2+ signalling pathway showed either a complete inhibition or a significant reduction of the vitellogenic process. These results were confirmed with the rescue capability of the A-23187 ionophore in those oocyte batches in which the IP3/Ca2+ pathway was inhibited. Taking our findings into account, we propose that the cAMP molecule binds Xepac protein enabling it to activate the IP3/Ca2+ pathway, which is necessary to start and maintain X. laevis vitellogenin uptake.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21989
Serrano, María de Los Angeles; Luque, Melchor Emilio; Sanchez, Sara Serafina del V.; Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis; Cambridge University Press; Zygote; 23; 1; 7-2013; 99-110
0967-1994
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21989
identifier_str_mv Serrano, María de Los Angeles; Luque, Melchor Emilio; Sanchez, Sara Serafina del V.; Xepac protein and IP3/Ca2+ pathway implication during Xenopus laevis vitellogenesis; Cambridge University Press; Zygote; 23; 1; 7-2013; 99-110
0967-1994
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0967199413000324
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/xepac-protein-and-ip3ca2-pathway-implication-during-xenopus-laevis-vitellogenesis/04B119C289520A30E49BC46E8F534D0E
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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