Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study
- Autores
- Albani, Clara Maria; Borgo, Jimena; Fabbri, Julia; Pensel, Patricia Eugenia; Fasciani, Lara; Hernandez, N.; Paladini, A.; Sülsen, Valeria Patricia; Elissondo, María Celina
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Cystic echinococcosis (CE) is a worldwide zoonotic disease caused by Echinococcus granulosus, which produces long-term infections in humans and animals. Available anti-parasitic treatment against CE is mostly limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has undesirable side effects and their efficacy is about 50%. Based on the problematic described, new treatment alternatives are urgently needed. Plants from the Stevia genus (Asteraceae) are a potential source of anti-protozoal and anti-microbial compounds. The aim of the present study was to evaluate the in vitro and in vivo efficacy of the Stevia aristata dichloromethane extract against E. granulosus. Viable and free protoscoleces or cysts were treated with 100, 50, 10 and 5 μg/ml of the extract. Viability assessment using the methylene blue exclusion test and scanning electron microscopy (SEM) (for protoscoleces) or evaluation of germinal layer collapse (for cysts) was performed. CF-1 mice (n=30) infected with E. granulosus were allocated into the following experimental groups (6 months post-infection): (1) Control, (2) ABZ 25 mg/kg, every 24 h for 30 days; (3) S. aristata 50 mg/kg, every 24 h for 23 days. At the end of the treatment the weight of the cysts was recorded and samples were analysed by SEM. Protoscoleces viability decreased quickly with 100 µg/ml, reaching 0% after 20 days of treatment. After 4 days of incubation, the collapse of the germinal layer was observed in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated with 50 and 100 µg/ml, respectively. Whilst ultrastructural damage was observed in the cysts obtained from S. aristata or ABZ treated mice, no significant differences in the weight of the cysts were obtained (P > 0.05). In conclusion, S. aristata treatment caused high protoscolicidal and cysticidal effects, but not significant reduction in the weight of the cysts in experimentally infected mice.
Fil: Albani, Clara Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Borgo, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fabbri, Julia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Pensel, Patricia Eugenia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Fasciani, Lara. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Hernandez, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; Argentina
Fil: Paladini, A.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Zoonosis Parasitarias; Argentina
Fil: Sülsen, Valeria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Elissondo, María Celina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Farmacología Experimental - Materia
-
Stevia aristata
Echinococcus granulosus
Phytotherapy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/156328
Ver los metadatos del registro completo
| id |
CONICETDig_10f480a12ef0dfca4d34adc2e1c42b7a |
|---|---|
| oai_identifier_str |
oai:ri.conicet.gov.ar:11336/156328 |
| network_acronym_str |
CONICETDig |
| repository_id_str |
3498 |
| network_name_str |
CONICET Digital (CONICET) |
| spelling |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo studyAlbani, Clara MariaBorgo, JimenaFabbri, JuliaPensel, Patricia EugeniaFasciani, LaraHernandez, N.Paladini, A.Sülsen, Valeria PatriciaElissondo, María CelinaStevia aristataEchinococcus granulosusPhytotherapyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cystic echinococcosis (CE) is a worldwide zoonotic disease caused by Echinococcus granulosus, which produces long-term infections in humans and animals. Available anti-parasitic treatment against CE is mostly limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has undesirable side effects and their efficacy is about 50%. Based on the problematic described, new treatment alternatives are urgently needed. Plants from the Stevia genus (Asteraceae) are a potential source of anti-protozoal and anti-microbial compounds. The aim of the present study was to evaluate the in vitro and in vivo efficacy of the Stevia aristata dichloromethane extract against E. granulosus. Viable and free protoscoleces or cysts were treated with 100, 50, 10 and 5 μg/ml of the extract. Viability assessment using the methylene blue exclusion test and scanning electron microscopy (SEM) (for protoscoleces) or evaluation of germinal layer collapse (for cysts) was performed. CF-1 mice (n=30) infected with E. granulosus were allocated into the following experimental groups (6 months post-infection): (1) Control, (2) ABZ 25 mg/kg, every 24 h for 30 days; (3) S. aristata 50 mg/kg, every 24 h for 23 days. At the end of the treatment the weight of the cysts was recorded and samples were analysed by SEM. Protoscoleces viability decreased quickly with 100 µg/ml, reaching 0% after 20 days of treatment. After 4 days of incubation, the collapse of the germinal layer was observed in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated with 50 and 100 µg/ml, respectively. Whilst ultrastructural damage was observed in the cysts obtained from S. aristata or ABZ treated mice, no significant differences in the weight of the cysts were obtained (P > 0.05). In conclusion, S. aristata treatment caused high protoscolicidal and cysticidal effects, but not significant reduction in the weight of the cysts in experimentally infected mice.Fil: Albani, Clara Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Borgo, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fabbri, Julia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Pensel, Patricia Eugenia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Fasciani, Lara. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Hernandez, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; ArgentinaFil: Paladini, A.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Zoonosis Parasitarias; ArgentinaFil: Sülsen, Valeria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Elissondo, María Celina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaLXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaAsociación Argentina de NanomedicinasAsociación Argentina de Farmacología ExperimentalFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156328Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study; LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 72-721669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:46Zoai:ri.conicet.gov.ar:11336/156328instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:46.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| title |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| spellingShingle |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study Albani, Clara Maria Stevia aristata Echinococcus granulosus Phytotherapy |
| title_short |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| title_full |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| title_fullStr |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| title_full_unstemmed |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| title_sort |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study |
| dc.creator.none.fl_str_mv |
Albani, Clara Maria Borgo, Jimena Fabbri, Julia Pensel, Patricia Eugenia Fasciani, Lara Hernandez, N. Paladini, A. Sülsen, Valeria Patricia Elissondo, María Celina |
| author |
Albani, Clara Maria |
| author_facet |
Albani, Clara Maria Borgo, Jimena Fabbri, Julia Pensel, Patricia Eugenia Fasciani, Lara Hernandez, N. Paladini, A. Sülsen, Valeria Patricia Elissondo, María Celina |
| author_role |
author |
| author2 |
Borgo, Jimena Fabbri, Julia Pensel, Patricia Eugenia Fasciani, Lara Hernandez, N. Paladini, A. Sülsen, Valeria Patricia Elissondo, María Celina |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Stevia aristata Echinococcus granulosus Phytotherapy |
| topic |
Stevia aristata Echinococcus granulosus Phytotherapy |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Cystic echinococcosis (CE) is a worldwide zoonotic disease caused by Echinococcus granulosus, which produces long-term infections in humans and animals. Available anti-parasitic treatment against CE is mostly limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has undesirable side effects and their efficacy is about 50%. Based on the problematic described, new treatment alternatives are urgently needed. Plants from the Stevia genus (Asteraceae) are a potential source of anti-protozoal and anti-microbial compounds. The aim of the present study was to evaluate the in vitro and in vivo efficacy of the Stevia aristata dichloromethane extract against E. granulosus. Viable and free protoscoleces or cysts were treated with 100, 50, 10 and 5 μg/ml of the extract. Viability assessment using the methylene blue exclusion test and scanning electron microscopy (SEM) (for protoscoleces) or evaluation of germinal layer collapse (for cysts) was performed. CF-1 mice (n=30) infected with E. granulosus were allocated into the following experimental groups (6 months post-infection): (1) Control, (2) ABZ 25 mg/kg, every 24 h for 30 days; (3) S. aristata 50 mg/kg, every 24 h for 23 days. At the end of the treatment the weight of the cysts was recorded and samples were analysed by SEM. Protoscoleces viability decreased quickly with 100 µg/ml, reaching 0% after 20 days of treatment. After 4 days of incubation, the collapse of the germinal layer was observed in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated with 50 and 100 µg/ml, respectively. Whilst ultrastructural damage was observed in the cysts obtained from S. aristata or ABZ treated mice, no significant differences in the weight of the cysts were obtained (P > 0.05). In conclusion, S. aristata treatment caused high protoscolicidal and cysticidal effects, but not significant reduction in the weight of the cysts in experimentally infected mice. Fil: Albani, Clara Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Borgo, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Fabbri, Julia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Pensel, Patricia Eugenia. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Fasciani, Lara. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Hernandez, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Farmacognosia; Argentina Fil: Paladini, A.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Zoonosis Parasitarias; Argentina Fil: Sülsen, Valeria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Elissondo, María Celina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR) Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Asociación Argentina de Nanomedicinas Asociación Argentina de Farmacología Experimental |
| description |
Cystic echinococcosis (CE) is a worldwide zoonotic disease caused by Echinococcus granulosus, which produces long-term infections in humans and animals. Available anti-parasitic treatment against CE is mostly limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has undesirable side effects and their efficacy is about 50%. Based on the problematic described, new treatment alternatives are urgently needed. Plants from the Stevia genus (Asteraceae) are a potential source of anti-protozoal and anti-microbial compounds. The aim of the present study was to evaluate the in vitro and in vivo efficacy of the Stevia aristata dichloromethane extract against E. granulosus. Viable and free protoscoleces or cysts were treated with 100, 50, 10 and 5 μg/ml of the extract. Viability assessment using the methylene blue exclusion test and scanning electron microscopy (SEM) (for protoscoleces) or evaluation of germinal layer collapse (for cysts) was performed. CF-1 mice (n=30) infected with E. granulosus were allocated into the following experimental groups (6 months post-infection): (1) Control, (2) ABZ 25 mg/kg, every 24 h for 30 days; (3) S. aristata 50 mg/kg, every 24 h for 23 days. At the end of the treatment the weight of the cysts was recorded and samples were analysed by SEM. Protoscoleces viability decreased quickly with 100 µg/ml, reaching 0% after 20 days of treatment. After 4 days of incubation, the collapse of the germinal layer was observed in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated with 50 and 100 µg/ml, respectively. Whilst ultrastructural damage was observed in the cysts obtained from S. aristata or ABZ treated mice, no significant differences in the weight of the cysts were obtained (P > 0.05). In conclusion, S. aristata treatment caused high protoscolicidal and cysticidal effects, but not significant reduction in the weight of the cysts in experimentally infected mice. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
| status_str |
publishedVersion |
| format |
conferenceObject |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/156328 Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study; LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 72-72 1669-9106 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/156328 |
| identifier_str_mv |
Anthelmintic activity of Stevia aristata extract on Echinococcus granulosus: in vitro and in vivo study; LXVI Annual Meeting of Sociedad Argentina de Investigación Clínica (SAIC); LXIX Annual Meeting of Sociedad Argentina de Inmunología (SAI); LIII Annual Meeting of Asociación Argentina de Farmacología Experimental (AAFE) y XI Annual Meeting of Asociación Argentina de Nanomedicinas (NANOMED-AR); Argentina; 2021; 72-72 1669-9106 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.reunionbiociencias.com.ar/wp-content/uploads/2021/11/Revista-Medicina-2021.pdf |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.coverage.none.fl_str_mv |
Nacional |
| dc.publisher.none.fl_str_mv |
Fundación Revista Medicina |
| publisher.none.fl_str_mv |
Fundación Revista Medicina |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
| _version_ |
1844613290412474368 |
| score |
13.070432 |