Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina

Autores
D´Astek, Beatriz; del Castillo, Lourdes Leonor; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martín; Deza, Natalia; Chinen, Isabel; Manfredi, Eduardo; Leotta, Gerardo Anibal; Masana, Marcelo; Rivas, Marta
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx 2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx 2c(vh-a) (seven humans and one bovine), and PT49-AREXH01.0022- stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
Fil: D´Astek, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: del Castillo, Lourdes Leonor. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Palladino, Pablo Martín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Deza, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Manfredi, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Masana, Marcelo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Materia
Stec
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80027
Acceder
id CONICETDig_105ee3648c7dfffcaf3ad187221c1aca
oai_identifier_str oai:ri.conicet.gov.ar:11336/80027
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in ArgentinaD´Astek, Beatrizdel Castillo, Lourdes LeonorMiliwebsky, ElizabethCarbonari, Claudia CarolinaPalladino, Pablo MartínDeza, NataliaChinen, IsabelManfredi, EduardoLeotta, Gerardo AnibalMasana, MarceloRivas, MartaStechttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx 2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx 2c(vh-a) (seven humans and one bovine), and PT49-AREXH01.0022- stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.Fil: D´Astek, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: del Castillo, Lourdes Leonor. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; ArgentinaFil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Palladino, Pablo Martín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; ArgentinaFil: Deza, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Manfredi, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Masana, Marcelo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaMary Ann Liebert2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80027D´Astek, Beatriz; del Castillo, Lourdes Leonor; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martín; et al.; Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 5; 5-2012; 457-4641535-3141CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2011.1062info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:06:37Zoai:ri.conicet.gov.ar:11336/80027instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:06:37.395CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
title Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
spellingShingle Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
D´Astek, Beatriz
Stec
title_short Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
title_full Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
title_fullStr Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
title_full_unstemmed Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
title_sort Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina
dc.creator.none.fl_str_mv D´Astek, Beatriz
del Castillo, Lourdes Leonor
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martín
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo Anibal
Masana, Marcelo
Rivas, Marta
author D´Astek, Beatriz
author_facet D´Astek, Beatriz
del Castillo, Lourdes Leonor
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martín
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo Anibal
Masana, Marcelo
Rivas, Marta
author_role author
author2 del Castillo, Lourdes Leonor
Miliwebsky, Elizabeth
Carbonari, Claudia Carolina
Palladino, Pablo Martín
Deza, Natalia
Chinen, Isabel
Manfredi, Eduardo
Leotta, Gerardo Anibal
Masana, Marcelo
Rivas, Marta
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Stec
topic Stec
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx 2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx 2c(vh-a) (seven humans and one bovine), and PT49-AREXH01.0022- stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
Fil: D´Astek, Beatriz. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: del Castillo, Lourdes Leonor. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Carbonari, Claudia Carolina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Palladino, Pablo Martín. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria. Instituto de Tecnología de Alimentos; Argentina
Fil: Deza, Natalia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Manfredi, Eduardo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Leotta, Gerardo Anibal. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Masana, Marcelo. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Rivas, Marta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
description Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx2/stx2c(vh-a) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx2 (20.8%) in human and stx 2c(vh-a) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx2/stx2c(vh-a) (12 humans and one bovine), PT4-AREXH01.0543-stx2/stx2c(vh-a) (one human and four bovines), PT2-AREXH01.0076-stx2/stx2c(vh-a) (one human and four bovines), PT49-AREXH01.0175-stx2/stx 2c(vh-a) (seven humans and one bovine), and PT49-AREXH01.0022- stx2/stx2c(vh-a) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80027
D´Astek, Beatriz; del Castillo, Lourdes Leonor; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martín; et al.; Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 5; 5-2012; 457-464
1535-3141
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80027
identifier_str_mv D´Astek, Beatriz; del Castillo, Lourdes Leonor; Miliwebsky, Elizabeth; Carbonari, Claudia Carolina; Palladino, Pablo Martín; et al.; Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina; Mary Ann Liebert; Foodborne Pathogens And Disease; 9; 5; 5-2012; 457-464
1535-3141
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/abs/10.1089/fpd.2011.1062
info:eu-repo/semantics/altIdentifier/doi/10.1089/fpd.2011.1062
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert
publisher.none.fl_str_mv Mary Ann Liebert
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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