An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter
- Autores
- Rivas, Gabriel Alejandro; Francioni, Julian; Sánchez, Mariana; Valdés la Hens, Danay; Semorile, Liliana Carmen; Delfederico, Lucrecia
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this work, a fermentation starter involving autochthonous strains obtained from an artisanal dry sausage from Colonia Caroya (Córdoba, Argentina) was formulated, with the aim of preserving the typicity that characterizes this artisanal meat product. Isolates of lactic acid bacteria (LAB) and coagulase-negative-cocci (CNC) were obtained, identified and typified using 16S rRNA gene sequences and RAPD-PCR reactions. Some technological and safety-related properties were also studied: 1-resistance to low pH and high salt concentration, 2-proteolytic and lipolytic activities, 3-antibiotic resistance against tetracycline, kanamycin, vancomycin and ampicillin, and 4-absence of histidine decarboxylase (hdc) gene. A bacterial consortium of one LAB (Latilactobacillus sakei UNQLs16) and five CNC selected strains with the best features was used to formulate a meat fermentation starter (UNQ-MFS), which was compared with a commercial one (C-MFS), in a pilot-scale sausage production. Certain technological parameters (pH and weight reduction, microbial counts, and final texture profiles) were assessed in both batches to ensure the appropriate development of the process. The strains implantation was followed using RAPD-PCR: L. sakei UNQLs16 showed dominance throughout the process and its implantation was confirmed in the batch inoculated with UNQ-MFS, whereas isolates obtained from the C-MFS were not detected in the batch inoculated with this commercial starter. The fermentative starter design with native strains is not only relevant for the regional producers, but also to expand the knowledge and increase the number and diversity of available strains capable of leading the fermentative processes, contributing to safety and quality of foodstuffs.
Fil: Rivas, Gabriel Alejandro. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Francioni, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Sánchez, Mariana. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Valdés la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
ARGENTINIAN SAUSAGE
COAGULASE NEGATIVE COCCI
DRY-FERMENTED SAUSAGE
LACTIC ACID BACTERIA
PILOT SCALE AUTOCHTHONOUS STARTER - Nivel de accesibilidad
- acceso embargado
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/220482
Ver los metadatos del registro completo
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An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starterRivas, Gabriel AlejandroFrancioni, JulianSánchez, MarianaValdés la Hens, DanaySemorile, Liliana CarmenDelfederico, LucreciaARGENTINIAN SAUSAGECOAGULASE NEGATIVE COCCIDRY-FERMENTED SAUSAGELACTIC ACID BACTERIAPILOT SCALE AUTOCHTHONOUS STARTERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work, a fermentation starter involving autochthonous strains obtained from an artisanal dry sausage from Colonia Caroya (Córdoba, Argentina) was formulated, with the aim of preserving the typicity that characterizes this artisanal meat product. Isolates of lactic acid bacteria (LAB) and coagulase-negative-cocci (CNC) were obtained, identified and typified using 16S rRNA gene sequences and RAPD-PCR reactions. Some technological and safety-related properties were also studied: 1-resistance to low pH and high salt concentration, 2-proteolytic and lipolytic activities, 3-antibiotic resistance against tetracycline, kanamycin, vancomycin and ampicillin, and 4-absence of histidine decarboxylase (hdc) gene. A bacterial consortium of one LAB (Latilactobacillus sakei UNQLs16) and five CNC selected strains with the best features was used to formulate a meat fermentation starter (UNQ-MFS), which was compared with a commercial one (C-MFS), in a pilot-scale sausage production. Certain technological parameters (pH and weight reduction, microbial counts, and final texture profiles) were assessed in both batches to ensure the appropriate development of the process. The strains implantation was followed using RAPD-PCR: L. sakei UNQLs16 showed dominance throughout the process and its implantation was confirmed in the batch inoculated with UNQ-MFS, whereas isolates obtained from the C-MFS were not detected in the batch inoculated with this commercial starter. The fermentative starter design with native strains is not only relevant for the regional producers, but also to expand the knowledge and increase the number and diversity of available strains capable of leading the fermentative processes, contributing to safety and quality of foodstuffs.Fil: Rivas, Gabriel Alejandro. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Francioni, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Sánchez, Mariana. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Valdés la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2023-10info:eu-repo/date/embargoEnd/2024-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/220482Rivas, Gabriel Alejandro; Francioni, Julian; Sánchez, Mariana; Valdés la Hens, Danay; Semorile, Liliana Carmen; et al.; An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter; Springer; Journal of Food Measurement and Characterization; 10-2023; 1-192193-41262193-4134CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11694-023-02189-9info:eu-repo/semantics/altIdentifier/doi/10.1007/s11694-023-02189-9info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:16Zoai:ri.conicet.gov.ar:11336/220482instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:16.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
title |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
spellingShingle |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter Rivas, Gabriel Alejandro ARGENTINIAN SAUSAGE COAGULASE NEGATIVE COCCI DRY-FERMENTED SAUSAGE LACTIC ACID BACTERIA PILOT SCALE AUTOCHTHONOUS STARTER |
title_short |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
title_full |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
title_fullStr |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
title_full_unstemmed |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
title_sort |
An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter |
dc.creator.none.fl_str_mv |
Rivas, Gabriel Alejandro Francioni, Julian Sánchez, Mariana Valdés la Hens, Danay Semorile, Liliana Carmen Delfederico, Lucrecia |
author |
Rivas, Gabriel Alejandro |
author_facet |
Rivas, Gabriel Alejandro Francioni, Julian Sánchez, Mariana Valdés la Hens, Danay Semorile, Liliana Carmen Delfederico, Lucrecia |
author_role |
author |
author2 |
Francioni, Julian Sánchez, Mariana Valdés la Hens, Danay Semorile, Liliana Carmen Delfederico, Lucrecia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
ARGENTINIAN SAUSAGE COAGULASE NEGATIVE COCCI DRY-FERMENTED SAUSAGE LACTIC ACID BACTERIA PILOT SCALE AUTOCHTHONOUS STARTER |
topic |
ARGENTINIAN SAUSAGE COAGULASE NEGATIVE COCCI DRY-FERMENTED SAUSAGE LACTIC ACID BACTERIA PILOT SCALE AUTOCHTHONOUS STARTER |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In this work, a fermentation starter involving autochthonous strains obtained from an artisanal dry sausage from Colonia Caroya (Córdoba, Argentina) was formulated, with the aim of preserving the typicity that characterizes this artisanal meat product. Isolates of lactic acid bacteria (LAB) and coagulase-negative-cocci (CNC) were obtained, identified and typified using 16S rRNA gene sequences and RAPD-PCR reactions. Some technological and safety-related properties were also studied: 1-resistance to low pH and high salt concentration, 2-proteolytic and lipolytic activities, 3-antibiotic resistance against tetracycline, kanamycin, vancomycin and ampicillin, and 4-absence of histidine decarboxylase (hdc) gene. A bacterial consortium of one LAB (Latilactobacillus sakei UNQLs16) and five CNC selected strains with the best features was used to formulate a meat fermentation starter (UNQ-MFS), which was compared with a commercial one (C-MFS), in a pilot-scale sausage production. Certain technological parameters (pH and weight reduction, microbial counts, and final texture profiles) were assessed in both batches to ensure the appropriate development of the process. The strains implantation was followed using RAPD-PCR: L. sakei UNQLs16 showed dominance throughout the process and its implantation was confirmed in the batch inoculated with UNQ-MFS, whereas isolates obtained from the C-MFS were not detected in the batch inoculated with this commercial starter. The fermentative starter design with native strains is not only relevant for the regional producers, but also to expand the knowledge and increase the number and diversity of available strains capable of leading the fermentative processes, contributing to safety and quality of foodstuffs. Fil: Rivas, Gabriel Alejandro. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Francioni, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina Fil: Sánchez, Mariana. Instituto Nacional de Tecnología Industrial; Argentina Fil: Valdés la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina Fil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
In this work, a fermentation starter involving autochthonous strains obtained from an artisanal dry sausage from Colonia Caroya (Córdoba, Argentina) was formulated, with the aim of preserving the typicity that characterizes this artisanal meat product. Isolates of lactic acid bacteria (LAB) and coagulase-negative-cocci (CNC) were obtained, identified and typified using 16S rRNA gene sequences and RAPD-PCR reactions. Some technological and safety-related properties were also studied: 1-resistance to low pH and high salt concentration, 2-proteolytic and lipolytic activities, 3-antibiotic resistance against tetracycline, kanamycin, vancomycin and ampicillin, and 4-absence of histidine decarboxylase (hdc) gene. A bacterial consortium of one LAB (Latilactobacillus sakei UNQLs16) and five CNC selected strains with the best features was used to formulate a meat fermentation starter (UNQ-MFS), which was compared with a commercial one (C-MFS), in a pilot-scale sausage production. Certain technological parameters (pH and weight reduction, microbial counts, and final texture profiles) were assessed in both batches to ensure the appropriate development of the process. The strains implantation was followed using RAPD-PCR: L. sakei UNQLs16 showed dominance throughout the process and its implantation was confirmed in the batch inoculated with UNQ-MFS, whereas isolates obtained from the C-MFS were not detected in the batch inoculated with this commercial starter. The fermentative starter design with native strains is not only relevant for the regional producers, but also to expand the knowledge and increase the number and diversity of available strains capable of leading the fermentative processes, contributing to safety and quality of foodstuffs. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10 info:eu-repo/date/embargoEnd/2024-04-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/220482 Rivas, Gabriel Alejandro; Francioni, Julian; Sánchez, Mariana; Valdés la Hens, Danay; Semorile, Liliana Carmen; et al.; An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter; Springer; Journal of Food Measurement and Characterization; 10-2023; 1-19 2193-4126 2193-4134 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/220482 |
identifier_str_mv |
Rivas, Gabriel Alejandro; Francioni, Julian; Sánchez, Mariana; Valdés la Hens, Danay; Semorile, Liliana Carmen; et al.; An indigenous bacterial consortium from Argentinean traditional dry sausages as a pilot-scale fermentation starter; Springer; Journal of Food Measurement and Characterization; 10-2023; 1-19 2193-4126 2193-4134 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11694-023-02189-9 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11694-023-02189-9 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/embargoedAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
embargoedAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268847690743808 |
score |
13.13397 |