CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat

Autores
de Tezanos Pinto, Felicitas; Corradi, Gerardo Raul; de la Hera, Diego Pablo; Adamo, Hugo Pedro
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
P-ATPases are membrane transporters energized by ATP. The subfamily of P5-ATPases is the least studied P-ATPases and the ion substrate specificity of the P5 subfamily is not known. Mutations of the human P5ATPase gene ATP13A2 has been shown to underlie a form of Parkinson disease (PD). We investigated the link between ATP13A2 and environmental factors related to PD development. Increasing concentrations of the synthetic polyamine analog paraquat induced a greater cytotoxic effect over CHO cells expressing ATP13A2. Paraquat-toxicity was associated with increased production of cellular reactive oxygen species and this increment was reversed by the natural polyamine spermidine. Acridine orange fluorescence intensity suggested that ATP13A2 induced the expansion of acidic vesicles that become more alkaline upon external addition of spermidine. Polyamine uptake is proposed to be initiated by a plasma membrane carrier followed by sequestration into acidic vesicles of the late endocytic compartment through an unidentified active mechanism; because ATP13A2 is located in lysosomes and late endosomes, our results open the possibility that ATP13A2 could be one of those active transporters capable of transporting polyamines like spermidine as well as its toxic analog paraquat.
Fil: de Tezanos Pinto, Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: de la Hera, Diego Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Adamo, Hugo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
P type ATPase
P5B-ATP13A2
Paraquat
Polyamines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269120
Acceder
id CONICETDig_0f69bf6e80804f1aa3bd016bc3296248
oai_identifier_str oai:ri.conicet.gov.ar:11336/269120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquatde Tezanos Pinto, FelicitasCorradi, Gerardo Raulde la Hera, Diego PabloAdamo, Hugo PedroP type ATPaseP5B-ATP13A2ParaquatPolyamineshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1P-ATPases are membrane transporters energized by ATP. The subfamily of P5-ATPases is the least studied P-ATPases and the ion substrate specificity of the P5 subfamily is not known. Mutations of the human P5ATPase gene ATP13A2 has been shown to underlie a form of Parkinson disease (PD). We investigated the link between ATP13A2 and environmental factors related to PD development. Increasing concentrations of the synthetic polyamine analog paraquat induced a greater cytotoxic effect over CHO cells expressing ATP13A2. Paraquat-toxicity was associated with increased production of cellular reactive oxygen species and this increment was reversed by the natural polyamine spermidine. Acridine orange fluorescence intensity suggested that ATP13A2 induced the expansion of acidic vesicles that become more alkaline upon external addition of spermidine. Polyamine uptake is proposed to be initiated by a plasma membrane carrier followed by sequestration into acidic vesicles of the late endocytic compartment through an unidentified active mechanism; because ATP13A2 is located in lysosomes and late endosomes, our results open the possibility that ATP13A2 could be one of those active transporters capable of transporting polyamines like spermidine as well as its toxic analog paraquat.Fil: de Tezanos Pinto, Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: de la Hera, Diego Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Adamo, Hugo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaPergamon-Elsevier Science Ltd2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269120de Tezanos Pinto, Felicitas; Corradi, Gerardo Raul; de la Hera, Diego Pablo; Adamo, Hugo Pedro; CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 3; 2-2012; 243-2480197-0186CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0197018612000034info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2012.01.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:57:19Zoai:ri.conicet.gov.ar:11336/269120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:57:19.897CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
title CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
spellingShingle CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
de Tezanos Pinto, Felicitas
P type ATPase
P5B-ATP13A2
Paraquat
Polyamines
title_short CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
title_full CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
title_fullStr CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
title_full_unstemmed CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
title_sort CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat
dc.creator.none.fl_str_mv de Tezanos Pinto, Felicitas
Corradi, Gerardo Raul
de la Hera, Diego Pablo
Adamo, Hugo Pedro
author de Tezanos Pinto, Felicitas
author_facet de Tezanos Pinto, Felicitas
Corradi, Gerardo Raul
de la Hera, Diego Pablo
Adamo, Hugo Pedro
author_role author
author2 Corradi, Gerardo Raul
de la Hera, Diego Pablo
Adamo, Hugo Pedro
author2_role author
author
author
dc.subject.none.fl_str_mv P type ATPase
P5B-ATP13A2
Paraquat
Polyamines
topic P type ATPase
P5B-ATP13A2
Paraquat
Polyamines
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv P-ATPases are membrane transporters energized by ATP. The subfamily of P5-ATPases is the least studied P-ATPases and the ion substrate specificity of the P5 subfamily is not known. Mutations of the human P5ATPase gene ATP13A2 has been shown to underlie a form of Parkinson disease (PD). We investigated the link between ATP13A2 and environmental factors related to PD development. Increasing concentrations of the synthetic polyamine analog paraquat induced a greater cytotoxic effect over CHO cells expressing ATP13A2. Paraquat-toxicity was associated with increased production of cellular reactive oxygen species and this increment was reversed by the natural polyamine spermidine. Acridine orange fluorescence intensity suggested that ATP13A2 induced the expansion of acidic vesicles that become more alkaline upon external addition of spermidine. Polyamine uptake is proposed to be initiated by a plasma membrane carrier followed by sequestration into acidic vesicles of the late endocytic compartment through an unidentified active mechanism; because ATP13A2 is located in lysosomes and late endosomes, our results open the possibility that ATP13A2 could be one of those active transporters capable of transporting polyamines like spermidine as well as its toxic analog paraquat.
Fil: de Tezanos Pinto, Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Corradi, Gerardo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: de la Hera, Diego Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Adamo, Hugo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description P-ATPases are membrane transporters energized by ATP. The subfamily of P5-ATPases is the least studied P-ATPases and the ion substrate specificity of the P5 subfamily is not known. Mutations of the human P5ATPase gene ATP13A2 has been shown to underlie a form of Parkinson disease (PD). We investigated the link between ATP13A2 and environmental factors related to PD development. Increasing concentrations of the synthetic polyamine analog paraquat induced a greater cytotoxic effect over CHO cells expressing ATP13A2. Paraquat-toxicity was associated with increased production of cellular reactive oxygen species and this increment was reversed by the natural polyamine spermidine. Acridine orange fluorescence intensity suggested that ATP13A2 induced the expansion of acidic vesicles that become more alkaline upon external addition of spermidine. Polyamine uptake is proposed to be initiated by a plasma membrane carrier followed by sequestration into acidic vesicles of the late endocytic compartment through an unidentified active mechanism; because ATP13A2 is located in lysosomes and late endosomes, our results open the possibility that ATP13A2 could be one of those active transporters capable of transporting polyamines like spermidine as well as its toxic analog paraquat.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269120
de Tezanos Pinto, Felicitas; Corradi, Gerardo Raul; de la Hera, Diego Pablo; Adamo, Hugo Pedro; CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 3; 2-2012; 243-248
0197-0186
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269120
identifier_str_mv de Tezanos Pinto, Felicitas; Corradi, Gerardo Raul; de la Hera, Diego Pablo; Adamo, Hugo Pedro; CHO cells expressing the human P5-ATPase ATP13A2 are more sensitive to the toxic effects of herbicide Paraquat; Pergamon-Elsevier Science Ltd; Neurochemistry International; 60; 3; 2-2012; 243-248
0197-0186
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0197018612000034
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2012.01.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782283123523584
score 12.982451

Publicaciones similares