A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole

Autores
Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.
Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; México
Fil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina
Materia
FASCIOLA HEPATICA
RESISTENCIA
TRICLABENDAZOL
ENZIMAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58561
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/58561
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to TriclabendazoleFernandez, VanesaCadenazzi, GabrielaMiranda Miranda, E.Larsen, Karen ElizabethSolana, Hugo DanielFASCIOLA HEPATICARESISTENCIATRICLABENDAZOLENZIMAShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; MéxicoFil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; ArgentinaOMICS2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58561Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-42157-7609CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7609.1000192info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-multienzyme-response-is-involved-in-the-phenomenon-of-fasciola-hepaticaresistance-to-triclabendazole-2157-7609-1000192.php?aid=62728info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:41Zoai:ri.conicet.gov.ar:11336/58561instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:41.968CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
title A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
spellingShingle A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
Fernandez, Vanesa
FASCIOLA HEPATICA
RESISTENCIA
TRICLABENDAZOL
ENZIMAS
title_short A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
title_full A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
title_fullStr A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
title_full_unstemmed A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
title_sort A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
dc.creator.none.fl_str_mv Fernandez, Vanesa
Cadenazzi, Gabriela
Miranda Miranda, E.
Larsen, Karen Elizabeth
Solana, Hugo Daniel
author Fernandez, Vanesa
author_facet Fernandez, Vanesa
Cadenazzi, Gabriela
Miranda Miranda, E.
Larsen, Karen Elizabeth
Solana, Hugo Daniel
author_role author
author2 Cadenazzi, Gabriela
Miranda Miranda, E.
Larsen, Karen Elizabeth
Solana, Hugo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv FASCIOLA HEPATICA
RESISTENCIA
TRICLABENDAZOL
ENZIMAS
topic FASCIOLA HEPATICA
RESISTENCIA
TRICLABENDAZOL
ENZIMAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.
Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; México
Fil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina
description The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58561
Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-4
2157-7609
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58561
identifier_str_mv Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-4
2157-7609
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7609.1000192
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-multienzyme-response-is-involved-in-the-phenomenon-of-fasciola-hepaticaresistance-to-triclabendazole-2157-7609-1000192.php?aid=62728
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS
publisher.none.fl_str_mv OMICS
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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