A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole
- Autores
- Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.
Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; México
Fil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina - Materia
-
FASCIOLA HEPATICA
RESISTENCIA
TRICLABENDAZOL
ENZIMAS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/58561
Ver los metadatos del registro completo
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A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to TriclabendazoleFernandez, VanesaCadenazzi, GabrielaMiranda Miranda, E.Larsen, Karen ElizabethSolana, Hugo DanielFASCIOLA HEPATICARESISTENCIATRICLABENDAZOLENZIMAShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ.Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; MéxicoFil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; ArgentinaOMICS2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58561Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-42157-7609CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7609.1000192info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-multienzyme-response-is-involved-in-the-phenomenon-of-fasciola-hepaticaresistance-to-triclabendazole-2157-7609-1000192.php?aid=62728info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:04Zoai:ri.conicet.gov.ar:11336/58561instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:04.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
title |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
spellingShingle |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole Fernandez, Vanesa FASCIOLA HEPATICA RESISTENCIA TRICLABENDAZOL ENZIMAS |
title_short |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
title_full |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
title_fullStr |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
title_full_unstemmed |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
title_sort |
A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole |
dc.creator.none.fl_str_mv |
Fernandez, Vanesa Cadenazzi, Gabriela Miranda Miranda, E. Larsen, Karen Elizabeth Solana, Hugo Daniel |
author |
Fernandez, Vanesa |
author_facet |
Fernandez, Vanesa Cadenazzi, Gabriela Miranda Miranda, E. Larsen, Karen Elizabeth Solana, Hugo Daniel |
author_role |
author |
author2 |
Cadenazzi, Gabriela Miranda Miranda, E. Larsen, Karen Elizabeth Solana, Hugo Daniel |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
FASCIOLA HEPATICA RESISTENCIA TRICLABENDAZOL ENZIMAS |
topic |
FASCIOLA HEPATICA RESISTENCIA TRICLABENDAZOL ENZIMAS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ. Fil: Fernandez, Vanesa. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cadenazzi, Gabriela. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina Fil: Miranda Miranda, E.. Centro Nacional de Investigación Disciplinaria en Parasitología; México Fil: Larsen, Karen Elizabeth. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Solana, Hugo Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Laboratorio de Biología Celular y Molecular; Argentina |
description |
The trematode Fasciola hepatica is the causative agent of a parasitic zoonosis known as fasciolosis, disease that affects humans and most species of domestic animals. Triclabendazole (TCBZ) is the most widely used fasciolicide,however, its indiscriminate use has led to the expression of anthelmintic resistance in the liver fluke.In the present work we evaluated ?in vitro? the microsomes activity of different xenobiotic metabolizing enzymes of Phase I Carboxylesterases (CE) and Phase II the cytosolic activity of Glutathione S-Transferase (GST),Glutathione peroxidase (GPx) and Glutathione Reductase (GSR) in adults of F. hepatica susceptible (Cullompton strain) and resistant (Sligo and Oberon strains) to triclabendazole. In this work is detected a multienzyme response involving at all the family of enzymes glutathione dependent. Carboxylesterases activity did not differ between the different strains tested not being involved in the resistance phenomenon. These results contribute to the understanding of the mechanisms referred to the phenomenon of resistance to TCBZ. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/58561 Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-4 2157-7609 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/58561 |
identifier_str_mv |
Fernandez, Vanesa; Cadenazzi, Gabriela; Miranda Miranda, E.; Larsen, Karen Elizabeth; Solana, Hugo Daniel; A Multienzyme Response is involved in the Phenomenon of Fasciola hepatica Resistance to Triclabendazole; OMICS; Journal of Drug Metabolism and Toxicology; 6; 4; 10-2015; 1-4 2157-7609 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7609.1000192 info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/a-multienzyme-response-is-involved-in-the-phenomenon-of-fasciola-hepaticaresistance-to-triclabendazole-2157-7609-1000192.php?aid=62728 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
OMICS |
publisher.none.fl_str_mv |
OMICS |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270030370177024 |
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13.13397 |