Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3

Autores
Plazas, Paola Viviana; Nicol, Xavier; Spitzer, Nicholas C.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activity-based competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding.
Fil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
Fil: Nicol, Xavier. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
Fil: Spitzer, Nicholas C.. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
Materia
Calcium Transients
Spontaneous Activity
Stochastic Expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3975
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3975
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3Plazas, Paola VivianaNicol, XavierSpitzer, Nicholas C.Calcium TransientsSpontaneous ActivityStochastic Expressionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activity-based competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding.Fil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados UnidosFil: Nicol, Xavier. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados UnidosFil: Spitzer, Nicholas C.. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados UnidosNational Academy Of Sciences2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3975Plazas, Paola Viviana; Nicol, Xavier; Spitzer, Nicholas C.; Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3; National Academy Of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 110; 4; 1-2013; 1524-15290027-8424enginfo:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/110/4/1524.fullinfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557035/info:eu-repo/semantics/altIdentifier/doi/10.1073%2Fpnas.1213048110info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:55Zoai:ri.conicet.gov.ar:11336/3975instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:55.379CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
title Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
spellingShingle Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
Plazas, Paola Viviana
Calcium Transients
Spontaneous Activity
Stochastic Expression
title_short Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
title_full Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
title_fullStr Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
title_full_unstemmed Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
title_sort Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3
dc.creator.none.fl_str_mv Plazas, Paola Viviana
Nicol, Xavier
Spitzer, Nicholas C.
author Plazas, Paola Viviana
author_facet Plazas, Paola Viviana
Nicol, Xavier
Spitzer, Nicholas C.
author_role author
author2 Nicol, Xavier
Spitzer, Nicholas C.
author2_role author
author
dc.subject.none.fl_str_mv Calcium Transients
Spontaneous Activity
Stochastic Expression
topic Calcium Transients
Spontaneous Activity
Stochastic Expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activity-based competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding.
Fil: Plazas, Paola Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
Fil: Nicol, Xavier. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
Fil: Spitzer, Nicholas C.. University of California at San Diego. Kavli Institute for Brain and Mind. Division of Biological Sciences. Neurobiology Section and Center for Neural Circuits and Behavior; Estados Unidos
description The role of electrical activity in axon guidance has been extensively studied in vitro. To better understand its role in the intact nervous system, we imaged intracellular Ca2+ in zebrafish primary motor neurons (PMN) during axon pathfinding in vivo. We found that PMN generate specific patterns of Ca2+ spikes at different developmental stages. Spikes arose in the distal axon of PMN and were propagated to the cell body. Suppression of Ca2+ spiking activity in single PMN led to stereotyped errors, but silencing all electrical activity had no effect on axon guidance, indicating that an activity-based competition rule regulates this process. This competition was not mediated by synaptic transmission. Combination of PlexinA3 knockdown with suppression of Ca2+ activity in single PMN produced a synergistic increase in the incidence of pathfinding errors. However, expression of PlexinA3 transcripts was not regulated by activity. Our results provide an in vivo demonstration of the intersection of spontaneous electrical activity with the PlexinA3 guidance molecule receptor in regulation of axon pathfinding.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3975
Plazas, Paola Viviana; Nicol, Xavier; Spitzer, Nicholas C.; Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3; National Academy Of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 110; 4; 1-2013; 1524-1529
0027-8424
url http://hdl.handle.net/11336/3975
identifier_str_mv Plazas, Paola Viviana; Nicol, Xavier; Spitzer, Nicholas C.; Activity-dependent competition regulates motor neuron axon pathfinding via PlexinA3; National Academy Of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 110; 4; 1-2013; 1524-1529
0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/110/4/1524.full
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557035/
info:eu-repo/semantics/altIdentifier/doi/10.1073%2Fpnas.1213048110
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy Of Sciences
publisher.none.fl_str_mv National Academy Of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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