Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

Autores
Carnevale, Silvana; Pantano, Maria Laura; Kamenetzky, Laura; Malandrini, Jorge Bruno; Soria, Claudia Cecilia; Velásquez, Jorge Néstor
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.
Fil: Carnevale, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pantano, Maria Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca; Argentina
Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca; Argentina
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Materia
Molecular Diagnosis
Dna Detection
Platyhelminth
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41572
Acceder
id CONICETDig_0e438ef55ca7a8a3a31809e2562acd1b
oai_identifier_str oai:ri.conicet.gov.ar:11336/41572
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular diagnosis of natural fasciolosis by DNA detection in sheep faecesCarnevale, SilvanaPantano, Maria LauraKamenetzky, LauraMalandrini, Jorge BrunoSoria, Claudia CeciliaVelásquez, Jorge NéstorMolecular DiagnosisDna DetectionPlatyhelminthhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.Fil: Carnevale, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pantano, Maria Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca; ArgentinaFil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca; ArgentinaFil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaDe Gruyter2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41572Carnevale, Silvana; Pantano, Maria Laura; Kamenetzky, Laura; Malandrini, Jorge Bruno; Soria, Claudia Cecilia; et al.; Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces; De Gruyter; Acta Parasitologica; 60; 2; 6-2015; 211-2171230-2821CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1515/ap-2015-0030info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/ap.2015.60.issue-2/ap-2015-0030/ap-2015-0030.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T09:10:51Zoai:ri.conicet.gov.ar:11336/41572instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 09:10:51.87CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
title Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
spellingShingle Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
Carnevale, Silvana
Molecular Diagnosis
Dna Detection
Platyhelminth
title_short Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
title_full Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
title_fullStr Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
title_full_unstemmed Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
title_sort Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces
dc.creator.none.fl_str_mv Carnevale, Silvana
Pantano, Maria Laura
Kamenetzky, Laura
Malandrini, Jorge Bruno
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author Carnevale, Silvana
author_facet Carnevale, Silvana
Pantano, Maria Laura
Kamenetzky, Laura
Malandrini, Jorge Bruno
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author_role author
author2 Pantano, Maria Laura
Kamenetzky, Laura
Malandrini, Jorge Bruno
Soria, Claudia Cecilia
Velásquez, Jorge Néstor
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Molecular Diagnosis
Dna Detection
Platyhelminth
topic Molecular Diagnosis
Dna Detection
Platyhelminth
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.
Fil: Carnevale, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pantano, Maria Laura. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Malandrini, Jorge Bruno. Universidad Nacional de Catamarca; Argentina
Fil: Soria, Claudia Cecilia. Universidad Nacional de Catamarca; Argentina
Fil: Velásquez, Jorge Néstor. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
description Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41572
Carnevale, Silvana; Pantano, Maria Laura; Kamenetzky, Laura; Malandrini, Jorge Bruno; Soria, Claudia Cecilia; et al.; Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces; De Gruyter; Acta Parasitologica; 60; 2; 6-2015; 211-217
1230-2821
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41572
identifier_str_mv Carnevale, Silvana; Pantano, Maria Laura; Kamenetzky, Laura; Malandrini, Jorge Bruno; Soria, Claudia Cecilia; et al.; Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces; De Gruyter; Acta Parasitologica; 60; 2; 6-2015; 211-217
1230-2821
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1515/ap-2015-0030
info:eu-repo/semantics/altIdentifier/url/https://www.degruyter.com/view/j/ap.2015.60.issue-2/ap-2015-0030/ap-2015-0030.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv De Gruyter
publisher.none.fl_str_mv De Gruyter
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1850505367145938944
score 13.214268

Publicaciones similares